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Effects of Embryo Developmental Stage and Superoxide Dismutase on the Survival of Frozen-Thawed Porcine Embryos by Open Pulled Straw (OPS) Method  

Lee Sang-Young (Biotechnology Division, Gyeongsangnam Province Advanced Swine Research Institute)
Yu Jae-Suck (Biotechnology Division, Gyeongsangnam Province Advanced Swine Research Institute)
Sa Soo-Jin (College of Animal Life Sciences, Kangwon National University)
Park Choon-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.30, no.1, 2006 , pp. 35-40 More about this Journal
Abstract
This study was performed to investigate the effects of embryo developmental stage and superoxide dismutase (SOD) on the survival of frozen-thawed porcine embryos by open pulled straw(OPS) method. Porcine IVF blastocysts were frozen-thawed by OPS method and cultured for 48 h under the existence of SOD. There are no significant differences in the proportions of normal morphology among the early, mid- and expanded blastoryst stages $(30.8{\sim}38.6%)$. After culture of embryos, the developmental rates to the expanded blastocyst stage(38.7%) were significantly higher than those of other stages (P<0.05). The proportions of expanded and hatched embryos were higher in medium with 1 unit/ml SOD than 0 and 10 units/ml of SOD. The result indicates that OPS method can use for the pig embryo cryopreservation, especially for the late stage blastocysts. SOD may can reduce the demage of frozen-thawed porcine embryos.
Keywords
OPS; SOD; Pig blastocyst; Survival ability;
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