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Effect of the Timing of Oocyte Activation on Development of Rat Somatic Cell Nuclear Transfer Embryos  

Roh, Sang-Ho (Department of Craniomaxillofacial Reconstructive Science, School of Dentistry and Dental Research Institute, Seoul National University)
Publication Information
Reproductive and Developmental Biology / v.29, no.4, 2005 , pp. 229-234 More about this Journal
Abstract
Methods for activation of reconstructed oocytes were examined for the production of nuclear transfer (NT) rat embryos using fetal neural stem cells as donor. Neural stem cells were isolated from Day 14.5 rat fetuses, and the oocytes for recipient cytoplasm were recovered from 4-week old Sprague Dawley rats. After enucleation and nuclear injection, the reconstructed oocytes were immediately exposed to activation medium consisting of 10 mM $SrCl_2$ for 4 h (immediate activation after injection; IAI), or cultured in vitro for $2\~3$ h before activation treatment (injection before activation; IBA). Pre-activated oocytes were also used for NT to test reprogramming potential of artificially activated oocytes. The oocytes were grouped as IIA (immediate injection after activation) and ABI (activation $2\~3$ h before injection). Following NT, the oocytes were cultured in vitro. Development of the NT embryos was monitored at 44 and 119 h after activation. The embryos in groups IAI, mA, and IIA were cleaved to the 2-cell stage at the rates of $36.6\%\;(15/41),\;39.5\%\;(17/43)\;and\;46.3\%$ (25/54), respectively. However, in the ABI group, only one embryo ($1.8\%$, 1/55) was cleaved after activation. After in vitro culture, two NT embryos from IAI group had developed to the morula stage $(4.9\%\cdot2/41)$. However, no morula or blastocyst was obtained in the other groups. These results suggest that immediate activation after injection (IAI) method may be used for the production of rat somatic cell NT embryos.
Keywords
Nuclear transfer; Oocyte activation; Rat;
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1 Cibelli JB, Stice SL, Golueke PL, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA, Robl JM (1998): Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 28:2095-2098   DOI   ScienceOn
2 Hirabayashi M, Kato M, Ishikawa A, Hochi S (2003): Factors influencing chromosome condensation and development of cloned rat embryos. Cloning and Stem cells 5:35-42   DOI   ScienceOn
3 Hyun S, Lee G, Kim D, Kim H, Lee S, Nam D, Jeong Y, Kim S, Yeom S, Kang S, Han J, Lee B, Hwang W (2003): Production of nuclear transfer-derived piglets using porcine fetal fibroblasts transfected with the enhanced green fluorescent protein. BioI Reprod 69:1060-1068   DOI   ScienceOn
4 Jiang J-Y, Mizuno S, Mizutani E, Miyoshi K, Kimura N, Sasada H, Sato E (2002):Nuclear transfer in rats using an established embryonic cell line and cumulus cells. J Reprod Dev 48:505-511   DOI   ScienceOn
5 Kato M, Hirabayashi M, Aoto T, Ito K, Ueda, Hochi S (2001): Strontium-induced activation regimen for rat oocytes in somatic cell nuclear transplantation. J Reprod Dev 47:407-413   DOI   ScienceOn
6 Keefer CL, Schuetz AW (1982): Spontaneous activation of ovulated rat oocytes during in vitro culture. J Exp ZooI 224:371-377   DOI   ScienceOn
7 Stice SL, Keefer CL, Matthews L (1994): Oocyte activation prior to blastomere fusion. Mol Reprod Dev 38:61-68   DOI   ScienceOn
8 Wakayama T, Perry ACF, Zuccotti M, Jolmson KR, Yanagirnachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374   DOI   ScienceOn
9 Kono T, Shinoda Y, Tsunoda Y (1988): Nuclear transplantation of rat embryos. J Exp ZooI 248:303-305   DOI
10 Campbell KHS, Ritchie WA, Wilmut I (1993): Nuclear cytoplasmic interactions during the first cell cycle of nuclear transfer reconstructed bovine embryos: implications for deoxyribonucleic acid replication and development. BioI Reprod 49:933-942   DOI   ScienceOn
11 Roh S, Malakooti N, Morrison JR, Trounson AO, Du ZT (2002): Parthenogenetic activation of rat oocytes and their development in vitro. Reprod Fertil Dev 15:135-143   DOI   ScienceOn
12 Charreau B Tesson L, Soulillou JP, Pourcel C, Anegon I (1996): Transgenesis in rats: technical aspects and models. Transgenic Res 5:223-234   DOI   ScienceOn
13 Wakayama T, Yanagimachi R (2001): Effect of cytokinesis inhibitors, DMSO and the timing of oocyte activation on mouse cloning using cumulus cell nuclei. Reproduction 122:49-60   DOI   PUBMED
14 Hayes E, Galea S, Verkuylen A, Pera M, Morrison J, Lacham-Kaplan O, Trounson AO (2001): Nuclear transfer of adult and genetically modified fetal cells of the rat. Physiol Genomics 5:193-203   DOI
15 Park KW, Lai L, Cheong HT, Im GS, Sun QY, Wu G, Day BN, Prather RS (2001): Developmental potential of porcine nuclear transfer embryos derived from transgenic fetal fibroblasts infected with the gene for the green fluorescent protein: comparison of different fusion/activation conditions. BioI Reprod 65:1681-1685   DOI   ScienceOn
16 Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KH (1997): Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science 278:2130-2133   DOI   PUBMED   ScienceOn
17 Iannaccone P, Taborn G, Garton R (2001): Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts. Zygote 9:135-143   DOI   ScienceOn
18 Wilmut I, Schieke AE, McWhir J, Kind AJ, Campbell KHS (1997): Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813   DOI   ScienceOn
19 Miyoshi K, Kono T, Niwa K (1997): Stage-dependent development of rat I-cell embryos in a chemically defined medium after fertilization in vivo and in vitro. BioI Reprod 56:180-185   DOI   ScienceOn
20 National Research Council (1996): Guide for care and use of laboratory animals, (Clark, J.D. ed.), National Academy of Sciences, Washington DC
21 Johe KK, Hazel TG, Muller T, Dugich-Djordjevic MM, McKay RD (1996): Single factors direct the differentiation of stem cells from the fetal and adult central nervous system. Genes Dev. 10:3129-3140   DOI   ScienceOn
22 Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C, Williams JL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade H, Godke RA, Gavin WG, Overstrom EW, Echelard Y (1999): Production of goats by somatic cell nuclear transfer. Nat Biotechnol 17:456-461   DOI   ScienceOn
23 Wells ON, Misica PM, Tervit HR (1999): Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. BioI Reprod 60:996-1005   DOI   ScienceOn
24 Zhou Q Renard J-P, Le Friec G, Brochard V, Beaujean N, Cherifi Y, Fraichard A, Cozzi J (2003): Generation of fertile cloned rats by regulating oocyte activation. Science 302:1179   DOI   ScienceOn