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Effects of Cryopreservation of Sperm and Embryos on fertilization, Development and Pregnancy in Int Application  

Min Sung-Hun (Department of Animal Science and Biotechnology, kyungpook National University)
Park Yong-Soo (Kyoungbuk Livestock Research Institute)
Park Young-Sok (Department of Animal Science and Biotechnology, kyungpook National University)
Publication Information
Reproductive and Developmental Biology / v.29, no.3, 2005 , pp. 193-199 More about this Journal
Abstract
The cryopreservation of germ cells, sperm and embryos, has been largely used to increase the effect of artificial reproductive techniques for human infertility, but the efficiency of germ cell cryopreservation has been conkoversial till now. Thus, the effect of the cryopreservation of human sperm used for ICSI and the effect of the cryopreservation of embryos produced by ICSI on fertilizatiof development and pregnancy were investigated. Sperm freezing did not affect fertilizatiort development and pregnancy rates. Also, there was no significant difference between ejaculated and testicular sperm in ferclizatiort development and pregnancy. Embryo freezing methods, slow freezing and vitrificatior did not differ each other in viability and pregnncy rates. However, ICSI embryo freezing significantly decreased pregnancy rate compared to fresh embryos freezing (p<0.05). In conclusiof this result suggested that cryopreservation of sperm for ICSI did not affect on the resulted embryo development and pregnancy, but ICSI embryo cryopreservation would significantly inhibit pregnancy.
Keywords
Cryopreservation; Sperm; Embryo; ICSI; Pregnancy;
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