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The Changes of Quantity and Quality of Proteins in Medium and Cytoplasm during In Vitro Maturation of Bovine Oocytes  

Park Y. S. (Kyongbuk Livestock Research Institute, College of Veterinary Medicine, Cheju National University)
Park H. Y. (College of Veterinary Medicine, Cheju National University)
Publication Information
Reproductive and Developmental Biology / v.29, no.3, 2005 , pp. 187-191 More about this Journal
Abstract
This study was designed to investigate the changes of quantity and quality of proteins in medium and cytoplasm during in vitro maturation of bovine oocytes. The total quantity of proteins in medium decreased from 0 to 4.5 hr, but increased from 13.5 to 18 hr after the onset of in vitro maturation. The total quantity of protein in cytoplasm increased from 0 to 4.5 hr, decreased from 4.5 to 9 hr, and increased after 18 hr after the onset of in vitro maturation. A total of 298 protein spots was detected on a gel of 2D SDS-PAGE form maturation medium. Among 28 protein spots expressed significant differences in their quantity, 8 proteins were identified by peptide mass fingerprinting (aldose reductase, alpha enolase, apolipoprotein A-1 precursor, 43kDa collectin precursor, heat shock 27kDa protein, plasminogen activator inhibitor-1 precursor, thrombospondin 1, transitional endoplasmic reticulum ATPase). Among total of 35 protein spots detected on gel of 2D SDS-PACE from oorytes cytoplasm, $\beta$-tubulin was identified by peptide mass fingerprinting.
Keywords
Bovine; In vitro maturation; Protein; 2-D electrophoresis;
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