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Cloned Placenta of Korean Native Calves Died Suddenly at Two Months after Birth Displays Differential Protein Expression  

Kim Hong Rye (Research Center for Transgenic Cloned Pigs. Chungnam National University)
Kang Jae Ku (Research Center for Transgenic Cloned Pigs. Chungnam National University)
Lee Hye Ran (Research Center for Transgenic Cloned Pigs. Chungnam National University)
Yoon Jong Taek (Genetic Engineering Institute, Hankyong National University)
Seong Hwan Hoo (National Livestock Research Institute, RDA)
Jung Jin Kwan (National Livestock Research Institute, RDA)
Park Chang Sik (Research Center for Transgenic Cloned Pigs. Chungnam National University)
Jin Dong Il (Research Center for Transgenic Cloned Pigs. Chungnam National University)
Publication Information
Reproductive and Developmental Biology / v.29, no.2, 2005 , pp. 63-68 More about this Journal
Abstract
Cloned calves derived from somatic cell nuclear transfer (SCNT) have been frequently lost by sudden death at 1 to 3 month following healthy birth. To address whether placental anomalies are responsible for the sudden death of cloned calves, we compared protein patterns of 2 placentae derived from SCNT of Korean Native calves died suddenly at two months after birth and those of 2 normal placentae obtained from AI fetuses. Placental proteins were separated using 2-Dimensional gel electrophoresis. Approximately 800 spots were detected in placental 2-D gel stained with coomassie-blue. Then, image analysis of Malanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between normal and SCNT placentae. In the comparison of normal and SCNT samples, 8 spots were identified to be up-regulated proteins and 24 spots to be down-regulated proteins in SCNT placentae, among which proteins were high mobility group protein HMG1, apolipoprotein A-1 precursor, bactenecin 1, tropomyosin beta chain, $H^+-transporting$ ATPase, carbonic anhydrase II, peroxiredoxin 2, tyrosine-rich acidic matrix protein, serum albumin precursor and cathepsin D. These results suggested that the sudden death of cloned calves might be related to abnormal protein expression in placenta.
Keywords
Cloned calves; Placenta; 2-D gel electrophoresis; Mass spectrometry;
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