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Cloning and Sequencing of Heterozygous PSS Gene in Pigs  

Yoo J. Y. (Dept. of Biotechnology, College of Animal Husbandry, Konkuk University)
Kim G. W. (Dept. of Animal Resource Science, College of Industrial Science, Kongju National University)
Lee J. W. (Dept. of Animal Resource Science, College of Industrial Science, Kongju National University)
Kim Y. B. (Dept. of Biotechnology, College of Animal Husbandry, Konkuk University)
Lee J. Y. (Dept. of Biotechnology, College of Animal Husbandry, Konkuk University)
Lee D. H. (Dept. of Biotechnology, College of Animal Husbandry, Konkuk University)
Lee H. J. (Dept. of Biotechnology, College of Animal Husbandry, Konkuk University)
Yoon J. M. (Dept. of Marine Life Science, College of Ocean Science and Technology, Kunsan National University)
Park H. Y. (Dept. of Biotechnology, College of Animal Husbandry, Konkuk University)
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Abstract
This study was carried out to analyze the structure of the gene related to porcine stress syndrome (PSS) through cloning from PSS heterozygous pigs and to examine hereditary type associated with PSS. The results obtained from the study were summarized as follows: Amino acid arginine and cysteine exist in dominant gene, N and harmfully recessive gene, n, respectively. It was confirmed that such genes exists as alleles in the sixth chromosome related to PSS. These allelic genes might be inherited according to Mendelian law.
Keywords
Porcine stress syndrome; Hetero; Ryanodine receptor; Cloning; DNA sequencing;
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