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Retrovirus Vector-Mediated Construction of Porcine Cell Lines Expressing Human PTH  

Jeong J. Y. (Department of Animal Science, Chungbuk National University)
Koo B. C. (Department of Animal Science, Chungbuk National University)
Kim N. H. (Department of Animal Science, Chungbuk National University)
Kwon M. S. (Department of Physiology, Catholic University of Daegu School of Medicine)
Park S. H. (Department of General Surgery, Catholic University of Daegu School of Medicine)
Kim T. O. (Department of Physiology, Catholic University of Daegu School of Medicine)
Publication Information
Reproductive and Developmental Biology / v.28, no.3, 2004 , pp. 197-202 More about this Journal
Abstract
Human PTH (parathyroid hormone) is known to be efficacious for curing osteoporesis. In this study, we attempted to construct genetically modified porcine cell lines that can ultimately be used for donor cells in nuclear transfer-mediated transgenesis. By using retrovirus vectors carrying tetracycline-regulatory expression system and WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, we could control PTH expression with tetracycline and boost the promoter activity. Considering that low or constitutive expression of the transgene has been one of major problems that needs to be solved in transgenic animal studies, our results could be helpful in successful production of transgenic pigs as bioreactors.
Keywords
hPTH; Tet-On system; retrovirus vector; WPRE;
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