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Effects of Exposure to Vitrification Solution on Maturation, Fertilization and Development of Immature Porcine Oocytes In Vitro  

Choi I. K. (Daegu Mirae Women’s Hospital)
Seok S. H. (College of Natural Resources, Daegu University)
Kim K. S. (Dheonan Yonam College)
Song H. B. (College of Natural Resources, Daegu University)
Publication Information
Reproductive and Developmental Biology / v.28, no.3, 2004 , pp. 173-179 More about this Journal
Abstract
This study was conducted to investigate the toxi-cological effects of different vitrification solution on development of immature porcine oocytes in vitro. Oocytes were exposed to EFS solution [40% ethylene glycol (EG) + 18% Ficoll + 0.3M sucrose], ES solution (5.5M EG + 1.0M sucrose) or GE solution [10% glycol (G) + 20% EG], and these oocytes were transferred to sucrose solution directly. Maturation rates were significantly (P<0.05) higher in the ES solution (44.5%) and control (57.6%) than in the EFS solution (38.8%) and GE solution (22.4%). No differences among three solution were found in fertilization rates. Cleavage rates was significantly (P<0.05) higher in the ES solution (47.1%) and control (65.9%) than in the EFS solution (21.9%) and GE solution (19.0%), but no difference among three solutions was found in the blastocyst formation rates. These results indicate that combination of EG and sucrose solutions had effects on development of immature porcine oocytes.
Keywords
Vitrification solution; Immature porcine oocyte; EFS; ES; GE;
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1 Bautista JA Kanagawa H (1998): Current status of vitrification of embryos and oocytes in domestic animals: Ethylene glycol as the emerging cryoprotectant of choice. Jpn J Vet Res 45:183-191
2 Dinnyes A Dai Y, Jiang S, Yang X (2000): High developmental rates of vitrified bovine oocytes following parthenogenetic activation, in vitro fertilization, and somatic cell nuclear transfer. Biol Reprod 63:513-518
3 Dochi O, Oshima K, Takenouchi N, Komatsu M (1998): Effect of exposure to cryoprotectant solutions in cleavage and subsequent development of immature bovine oocyte in vitro. Theriogenology 49(1):167 (Abst.)
4 Eric S, Surry J (1990): Successful ultrarapid freezing of unfertilized oocytes. J of in vitro Fertil Embryo Transfer 7(5):262   DOI   ScienceOn
5 Kasai M, Komi JH, Sakurai T, Machida T (1990): A simple methods for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fert 89:91-97   DOI   ScienceOn
6 Kasai M, Song HB (2003): Cryopreservation and cryobiology of mammalian oocytes. Deage University Life Sci Res 1(3):265-275
7 Kashiwazaki N, Shino M (2001): Ability of in vitro manipulated porcine embryos to develop to piglets. J Reprod Dev 47(suppl):S35-S39
8 Leibo SP, Oda K (1993): High survival of mouse zygote and embryos cooled rapidly or slowly in ethylene glycol plus polyvinylpyrrolidone. CryoLetters 14:133-144
9 Papis K, Avery B, Holm P, Callesen H, Greve T (1995): The effect of vitrification solution, equilibration time, and direct dilution method in survivability or vitrified bivine in vitro matures oocytes. Theriogenology 43:293(Abst.)
10 Quinn P, Kerin J, Stone B, Wilson L (1986): Successful cryopreservation of human oocytes. In:42nd Ann Mtg Am Fertil Soc and 18th Ann Mtg Canadian Fertil Androl Soc Abstr 72(Abst)
11 Saha S, Rajamahendran R, Boediono A, Sumantri C, Suzuki T (1996): Viability of bovine blastocysts obtained after 7, 8 or 9 days of culture in vitro following vitrification and one-step dehydration. Theriogenology 46:331-343   DOI   ScienceOn
12 Schellander K, Peli J, Schmoll F, Brem G (1994): Effects of cryopreservation and carbohydrates on freezing of matured and unmatured bovine oocytes. Theriogenology 23:909-915
13 Tachikawa S, Otoi T, Kondo S, Machida T, Kasai M (1993): Successful vitrification of bovine blastocyst, derived by in vitro maturation and fertilization. Mol Reprd Dev 34:66-271
14 Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen A, Greve T, Callesen H (1998): Open pulled straw (OPS) vitrification : a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51:53-58.   DOI   ScienceOn
15 Yang NS, Lu KH, Gordon I, Polge C (1992): Vitrification of blastocysts produced in vitro. Theriogenology 37:326   DOI   ScienceOn
16 Carroll J, Warnes GM, Matthews CD (1990): Freezethaw-induced changes of the zona pellucida explains decreased rates of ferilization in frozen-thawed mouse oocytes. J Reprod Fert 90:547-553   DOI   ScienceOn
17 Massip A, Mermillod P, Dinnyes A (1995): Morphology and biochemistry of in vitro produced bovine embryos : implication for their cryopreservation. Hum Reprod 10:3004-3011   PUBMED
18 Petter RM, Wells KD (1993) Culture of pig embryos. J Reprod Fertil Suppl 48:61-73
19 Le Gal F, Massip A (1999): Cryopreservation of cattle oocytes : effects of meiotic stage, cycloheximide treatment, and centrifugation procedure. Cryobiology 38:290-300   DOI   ScienceOn
20 Lane M, Schoolcraft WE, Gardner, DK (1999): Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique. Fertil Steril 72:1073-1078   DOI   PUBMED   ScienceOn
21 Abeydeera LR, Day BN (1997): In vitro penetration of pig oocytes in a modified tris-buffered medium: effect of BSA caffeine and calcium. Theriogenology 48: 537-544   DOI   ScienceOn
22 Otoi T, Yamamoto K, Suzuki T (1995): In vitro fertilization and development of immature and mature bovine oocytes cryopreserved with ethylene glycol and sucrose. Cryobiology 32:455-460   DOI   ScienceOn
23 김상근, 이봉구, 이규승 (1991): 소수정란의 초급속동결에 관한 연구. I. 소수정란의 완만 및 초급속동결후의 생존성에 관한 연구. 한국가축번식학회지 15(2):133-139
24 Vajta G, Rindom N, Peura TT, Holm P, Greve T, Callesen H (1999): The effect of media, serum and temperature on in vitro survival of bovine blastocysts after open pulled straw(OPS) vitrification. Theriogenology 52:939-948   DOI   ScienceOn
25 Diedrich K, AI Hasani S, Van der Ven H, Krebs D (1986): Successful in vitro fertilization of frozenthawed rabbit and human oocytes. J in vitro Fertil Embryo Transfer 3:65   DOI   ScienceOn
26 Hochi S, Akira K, Ken K, Akira H (1987): In vitro fertilizing ability of bovine ccoyte frozen-thawed at immature, maturing and maturestage. J Mamm Ova Res 14:61-65   DOI   ScienceOn
27 Hochi S, Fujimoto T, Choi Y, Braun J, Oguri N (1994): Cryopreservation of equine oocytes. Biol Reprod 6:1495-1501
28 Landa V, Tepla O (1990): Cryopreservation of mouse 8-cell embryos in microdrops. Folia Biol 36:153-158
29 Zhu S, Yoshizawa M, Muramatsu S (1998): Analysis of fertilizability of bovine oocytes cryopreserved in various cryoprotectants after in-vitro maturation and their chromosome at the first cleavage division. J Mamm Ova Res 15:37-42   DOI   ScienceOn
30 Cameron RD, Beebe LF, Blackshaw AW, Higgins A Nottle MB (2000): Piglets born from vitrified early blastocysts using a simple technique. Aust Vet J 78: 195-196
31 Vajta G, Booth PJ, Holm P, Greve T, Callesen H (1997) Successful vitrification of early stage bovine in vitro produced embryos with the open pulled straw(OPS) method. Cryo-Letters 18:191-195
32 Wall R (1996): Transgenic livestock ; Progress and prospects for the future. Theriogenology 45:57-68
33 Prather RS (2000): Pigs if pigs. Science 289:1886-1887
34 Dorbrinsky JR (1996): Cellular approach to cryopreservation of embryos. Theriogenology 45:17-26
35 Glenister PH, Wood MI, Kirby C, Whittingham DG (1987): Incidence of chromosome anomalies in firstcleavage mouse embryos obtained from frozenthawed fertilized oocytes in vitro. Gamete Res 16: 205-216   DOI   PUBMED
36 Martino A, Songsasen N, Leiba SP (1996): Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol Reprod 54: 1059-1069   DOI   ScienceOn
37 Herrler A, Rath D, Nieman H (1991): Effect of cryoprotectant on fertilization and cleavage of bovine oocytes in vitro. Theriogenology 35: 212 (Abst.)
38 Kasai M (1996): Simple and efficient methods for vitrification of mammalian embryos. Anim Reprod 42:67-75
39 Mahmoudzadeh AR, Van Soom A, Bols P, Ysebaert MT, De Kruif A (1995): Optimization of a simple vitrification procedure of bovine embryos produced in vitro: effect of developmental stage, two-step addition of cryoprotectant and sucrose dilution on embryonic survival. J Reprod Fert 103: 33-39   DOI   ScienceOn
40 Pieterse MC, Vos PLAM, Kruip ThAM, Wurth YA, Van Benden ThH, Willemse AH, Taneme MAM (1991): Transvaginal ultrasound guided follicular aspiration of bovine oocyte. Theriogenology 53:19-24
41 최인경, 송해범 (1998): 각 성숙단계에서 동결, 융해한 돼지 난포란의 발달능력에 관한 연구. 한국가축번식학회지 22(4): 319-329
42 Carroll J, Warnes GM, Matthews CD (1989): Increase in diginy explains polyploidy after in-vitro fertilization of frozen-thawed mouse oocytes. J Reprod Fert 85:489-494.   DOI   ScienceOn
43 AI-Hasani S, Diedrich K, Van der van H, Reinecke A Krebs D (1987): Cryopreservation of human oocytes. Human Reprod 2:695-700   DOI
44 Otoi T, Tachikawa S, Kondo S, Suzuki T (1993): Developmental capacity of bovine oocytes frozen in different cryoprotectants. Theriogenology 40: 801-807   DOI   ScienceOn
45 Park SP, Kim EY, Kim DI (1999): Simple, efficient and successful vitrification of bovine blastocysts using electron microscope grids. Human Reprod 14:2838-2843   DOI