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Expression of Arachidonate-Preferring Acyl-CoA Synthetase 4 in the Mouse Uterus during Pregnancy  

이상미 (전남대학교 농업생명과학대학, 농업과학기술연구소, 동물자원학부)
박효영 (전남대학교 농업생명과학대학, 농업과학기술연구소, 동물자원학)
정영희 (전남대학교 농업생명과학대학, 농업과학기술연구소, 동물자원학)
문승주 (전남대학교 농업생명과학대학, 농업과학기술연구소, 동물자원학)
강만종 (전남대학교 농업생명과학대학, 농업과학기술연구소, 동물자원학부)
Publication Information
Reproductive and Developmental Biology / v.28, no.2, 2004 , pp. 89-94 More about this Journal
Abstract
This study was conducted to determine expression of acyl-CoA synthetase 4(ACS4), which is involved in converts arachidonic acid to postaglandins, in the mouse uterus during pregnancy. In arachidonic acid metabolism, acyl-CoA synthetase plays a key role in the esterification of free arachidonic acid into membrane phospholipids. Following its release by the action of calcium dependent phospholipases, free arachidonic acid is believed to be rapidly converted to arachidonoyl-CoA and reesterified into phospholipids in order to prevent excessive synthesis of prostaglandins. Here we demonstrate that ACS4 gene are differentially regulated in the peri-implatation mouse uterus. During the preimplantation period(days 0.5∼3.5), the ACS4 gene was expressed in the uterus until day 3.5 after which the expression was downregulated. The expression of cPLA2, COX1, and COX2 gene was similar to that of ACS4 gene in the preimplantation periods. However expression levels of COX1 gene show much variation on the various days of pregnancy examined. These data, suggest that ACS4 expression in preimplantation period is involved in initial attachment reaction with cPLA2, COX1, and COX2 gene.
Keywords
Acyl-CoA synthetase; cPLA2; COX1; COX2; Prostaglandins; Mouse uterus;
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