Browse > Article

Developmental Potential of Bovine Nuclear Transfer Embryos Cultured in Serum-free Medium  

Lee, S. K (Animal Biotechnology Division, National Livestock Research Institute. RDA., Animal Resources Research Center, Konkuk University)
Kim, D. H. (Animal Biotechnology Division, National Livestock Research Institute. RDA.)
G. S. Im (Animal Biotechnology Division, National Livestock Research Institute. RDA.)
B. C. Yang (Animal Biotechnology Division, National Livestock Research Institute. RDA.)
Park, H. S. (Animal Biotechnology Division, National Livestock Research Institute. RDA.)
W. K. Chang (Animal Biotechnology Division, National Livestock Research Institute. RDA.)
Lee, H. T. (Animal Resources Research Center, Konkuk University)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.4, 2003 , pp. 339-347 More about this Journal
Abstract
The purpose of this study was to investigate the development of bovine nuclear transfer (NT) embryos cultured in serum-free conditions. Bovine NT embryos cultured in various culture conditions were compared blastocyst development, total cell number and apoptosis using TUNEL assay. In experiment 1, blastocyst rates of NT embryos were significantly higher (P<0.01) in FBS (22.0％) and BSA (26.6％) groups than in PVA (6.3％) group. Total cell number was significantly higher in FBS (78.4$\pm$19.4) and BSA (90.9$\pm$29.1) groups than in PVA group (46.0$\pm$0.0). Apoptotic cell number was significantly fewer in FBS (3.1$\pm$1.4) and BSA (1.7$\pm$1.4) groups than in PVA group (7.0$\pm$20.0) However, all of results were not different between the FBS and BSA group. In experiment 2, blastocyst rates of NT embryos were significantly higher (P<0.05) in fatty acid free-BSA (FAF-BSA) group (26.8％) than in fraction V-BSA group (11.2％). Total cell number were somewhat higher in FAF-BSA group (89.8$\pm$30.7) than in fraction V-BSA group (88.1$\pm$19.3). Apoptotic cell number were somewhat fewer in FAF-BSA (1.7$\pm$1.5) group than in fraction V-BSA group (4.2$\pm$2.9). These findings suggest that serum free condition were effective for the in vitro development of bovine NT embryos. Therefore, we concluded that fatty acid free-BSA has beneficial effect in development bovine NT embryos and can be use as a serum substitute.
Keywords
Nuclear transfer; FBS; BSA; PVA; Apoptosis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brison, D. and Schultz, R. 1998. Increased incidence of apoptosis in transforming growth factora-deficient mouse blastocyst. BioI. Reprod., 59:136-144   DOI   ScienceOn
2 Byrne, A., Southgate, J., Brison, D. and Leese, H. J. 1999. Analysis of apoptosis in the pre-implantation bovine embryo using TUNEL. J. Reprod. Fetil., 117:97-105.   DOI
3 Jursicova, A., Varmuza, S. and Casper, R. F. 1996. Programmed cell death and human embryo fragmentation. Mol. Hum. Reprod., 2:93-98
4 Langendonk, A., Auquier, P., Donnay, I. and Massip, A. 1996. Acceleration of in vitro bovine embryo development in the presence of fetal calf serum. Theriogenology, 45:194 abstr
5 Massip, A., Mermillod, P., Langendonkt, A., Reichenbach, H. D., Lonergan, P., Berg, U., Carolan, C. and Roover, R. 1996 Calving outcome following transfer of embryos produced in vitro in different condictions. Amim. Reprod. Sci., 44:1-10
6 Pinyopummintr, T. and Bavister, B. D. 1996. Effect of amino acids on development in vitro of cleavage stage bovine embryos into blastocyst. Reprod. Fertil. Dev., 8:835-841   DOI   ScienceOn
7 Rizos, D., Gutierrez-Adan., Garnelo, P., Fuente, J., Boland, M. and Lonergan, P. 2003. Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression. BioI. Reprod., 68:236-243
8 Sinclair, K. D., Young, L. E., Wilmut, I. and McEvoy, T. G. 2000. In-utero overgrowth in ruminants following embryo culture: lessons from mice and warming to men. Hum. Reprod., 15 (suppl) 5:68-86
9 Hill, J. R., Winger, Q. A., Looney, C. R., Thompson, J. A. and Westhusin, M. E. 2000. Development rates of mail bovine nuclear transfer embryos derived from adult and fetal cells. Biol. Reprod., 62:1135-40   DOI   ScienceOn
10 Carolan, C., Lonergan, P., Van Langendonckt, A. and Mermillod, P. 1995. Factors affecting bovine embryos development in Synthetic Oviduct Fluid following oocyte maturation and fertilization in vitro. Theriogenology, 43:1115-1128
11 Pampfer, S., Vanderheyden, I., McCracken, J., Vasela, J. and Hertogh, R. D. 1997. Increased cell death in rat blastocysts exposed to maternal diabetes in utero and to high glucose or tumour necrosis factor-a in vitro. Development, 124: 4827-4836   PUBMED
12 Schnieke, A. E., Kind, A. J., Ritchie, W. A., Mycock, Scott, A. R., Ritchie, M., Willmut, I., Colmon, A. and Campbell, K. H. 1997. Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts. Science, 278:2130-2133   DOI   PUBMED   ScienceOn
13 Wells, D. N., Misica, P. M. and Tervit, H. R. 1999. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. BioI. Reprod., 60:996-1005   DOI   ScienceOn
14 Wilmut, I., Schnieke, A. E., McWhir, J., Kind, A. J. and Cambell K. H. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature, 385:810-3
15 Gutierrez-Adan, A., Lonergan, P., Rizos, D., Ward, F. A., Boland, M. P., Pintado, B. and Fuente, J. 2001. Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryos. Theriogenology, 55:1117-1126
16 Takahashi, Y. and First, N. L. 1992. In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate amino acids and vitamin. Theriogenology, 37:963-978   DOI   ScienceOn
17 Langendonckt, A. V., Donnay, I., Schuurbiers, N., Auquier, P., Carolan, C., Massp, A. and Dessy, F. 1997. Effect of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium. J. Reprod. Fertil., 109:87-93
18 Thompson, J. G., Gardner, D. K., Pugh, P. A., McMillan, W. H. and Tervit, H. R. 1995. Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos. BioI. Reprod., 53 :1385-1391   DOI   ScienceOn
19 Thompson, J. G., Allen, N. W., McGowan, L. T., Bell, A. C. S., Lambert, M. G. and Tervit, H. R. 1998. Effect of delayed supplementation of fetal calf serum to culture medium on bovine embryos development in vitro and following transfer. Theriogenology, 49:1239-1249   DOI   ScienceOn
20 Flood, L. P. and Shirley, B. 1991. Reduction of embryostoxicity by protein in embryos culture media. Mol. Reprod. Dev., 30:226-231   DOI   ScienceOn
21 Gjorret, J., Wengle, J., King, W. A., Schllander, K. and Hyttel, P. 2002. Occurrence of apoptosis in bovine embryos reconstructed by nuclear transfer or derived in vivo. Theriogenology, 57:495
22 Hill, J. R., Roussel, A. J., Cibelli, E. J., Hooper, N. L. and Miller, M. W. 1999. Clinical and pathologic features of cloned transgenic claves and fetuses. Theriogenology, 51:1451-65   DOI   ScienceOn
23 Im, G. S., Yang, B. S., Yang, B. S., Chang, W. K., Yi, Y. J. and Park, C. S. 2001. Effect of cell cycle stage on the development of embryos produced by cumulus cell nuclear transfer in Hanwoo (Korea cattle). Asian-Ausu. J. Anim. Sci., 14:759-764   DOI
24 Young, L. E. and Fairburn, H. R. 2000. Improving the safety of embryos technologies: possible role of genomic imprinting. Theriogenology, 53:627-48
25 Gardner, D. and Lane, M. 1999. Development of Viable mammal embryos in vitro: Evolution of sequential media. In 'Handbook of Principles of Cloning,' 9nd Ed. (Gardner DK and Lane M, eds), pp. 188-208. AP press
26 Liu, Z. and Foote, R. H. 1995. Development of bovine embryos in KSOM with added superoxide dismutase and trurine and five and twenty-five percent $O_2$. BioI. Reprod., 53 : 786-790   DOI   ScienceOn
27 Van Soon, A., Ysebaert, M. and Kruif, A. D. 1997. Relationship between timing of development, morula morphology and cell allocation to inner cell mass and trophectoderm in in vitro produced embryos. Mol. Reprod. Dev., 47:47-56   DOI   ScienceOn
28 Wakayama, T., Perry, A. C., Zuccotti, M., Johson, K. R. and Yanagimachi, R. 1998. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394:369-74
29 Edwards, L., Batt, P. A., Gandolfi, F. and Gardner, D. K. 1997. Modifications made to culture medium by bovine oviduct epithelial cells: changes to carbohydrates stimulate bovine embryos development. Mol. Reprod. Dev., 46: 146-154   DOI   ScienceOn
30 Kato, Y., Sotomaru, Y., Kurokawa, K., Kato, J., Doguchi, H., Yasue, H. and Tsunoda, Y. 1998. Eight Calves Cloned from Somatic Cells of a single Adult. Science, 282: 2095- 2098
31 Rosenkrans, C. F. and First, N. L. 1994. Effect of free amino acid and vitamins on cleavage and developmental rate of bovine zygotes in vitro. J. Anim. Sci., 71:434-437
32 Lazzari, C., Wrenzycki, C., Herrmann, D., Duchi, R., Kruip, T., Niemann, H. and Galli, C. 2002. Cellular and molecular deviations in bovine In vitro-produced embryos are related to the large offspring syndrome. BioI. Reprod., 67:767-775   DOI   ScienceOn
33 Levy, R. R., Cordonier, H., Czyba, J. C. and Gocrin, J. E. 2001. Apoptosis in preimplantation mammalian embryo and genetics. Ital. J. Anat. Embryo., 106(suppl 2):101-108
34 Carolan, C., Lonergan, P., Khatir, H. and Mermillod, P. 1996. In vitro production of bovine embryos using individual oocytes Mol. Reprod. Dev., 45:145-150   DOI   ScienceOn
35 Eggan, K., Akutsu, H., Loring, J., Jackson-Grusby, Klemm, M. and Rideout, W. M. 2001. Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc. Natl. Acad. Sci. USA., 98;6209-14
36 Wagtendonk-de., Leeu, A. M., Mullaart, E., Roos, A. P. W., Merton, J. S., Den Dass, J. H. G., Kemp, B. and Ruigh, L. 2000. Effect of different reproduction techniques: AI, MOET or IVP, health and welfare of bovine offspring. Theriogenology, 53:575-597   DOI   ScienceOn
37 Feugang, J. M., Roover, D. E., Leonard, S., Dessy, F. and Donnay, I. 2002. Kinetics of apoptosis in preimplantation bovine embryos produced in vitro and in vivo. Theriogenology, 57:494