Effects of Changes in Glycosylation Sites on Secretion of Recombinant Human Erythropoietin in Cultured CHO Cells |
Lee, H. G
(Animal Biotechnology Division, NLRI, RDA)
Lee, P. Y. (Animal Biotechnology Division, NLRI, RDA) Lee, Y. K. (Animal Biotechnology Division, NLRI, RDA) Kim, S. J. (Animal Biotechnology Division, NLRI, RDA) H. K. Chung (Animal Biotechnology Division, NLRI, RDA) M. K. Seo (Animal Biotechnology Division, NLRI, RDA) Park, J. K. (Animal Biotechnology Division, NLRI, RDA) K. S. Min (Graduate School of Bio & Information Technology, Hankyong National University) W. K. Chang (Animal Biotechnology Division, NLRI, RDA) |
1 | Delorme Delorme, E., Lorenzini, T., Giffin, J., Martin, F., Jacobsen, F., Boone, T. and Elliott, S. 1992. Role of glycosylation on the secretion and biological activity of erythropoietin. Biochemistry 20;31(41):9871-9876 |
2 | Ellioitt, S., Lorenzini, T., Chang, D., Barzilay, J. and Delorme, E. 1997. Mapping of the active site of recombinant human erythropoietin. Blood 89:493-502 |
3 | Kawasaki, N., Haishima, Y., Ohta, M., Itoh, S., Hyuga, M., Hyuga, S. and Hayakawa, T. 2001. Structural analysis of sulfated N-linked oligosaccharides in erythropoietin. Glycobiology 11: 1043-1049 |
4 | Fisher, J. W. 1997. Erythropoietin: physiologic and pharmacologic aspects. Proc. Soc. Exp. BioI. Med. 216:358-369 DOI |
5 | Powell, J. S., Berkner, K. L., Lebo, R. V. and Adamson, J. W. 1986. Human erythropoietin gene: high level expression in stably transfected mammalian cells and chromosome localization. Proc. Natl. Acad. Sci. USA. 83(17):6465-6469 DOI ScienceOn |
6 | Shoemaker, C. B. and Mitsock, L. D. 1986. Murine EPO gene : cloning, expression and human gene homology. Mol. Cell BioI. 6:849-858 DOI |
7 | Macmillan, D., Bill, R. M., Sage, K. A., Fern, D. and Flitsch, S. L. 2001. Selective in vitro glycosylation of recombinant proteins: semisynthesis of novel homogeneous glycoforms of human erythropoietin. Chem. BioI. 8:133-145 DOI ScienceOn |
8 | Kitagawa, Y., Sano, Y., Veda, M., Higashio, K., Narita, H., Okano, M., Matsumoto, S. and Sasaki, R. 1994. N-glycosylation of erythropoietin is critical for apical secretion by Madin-Darby canine kidney cells. Experimental Cell Research 213:449-457 |
9 | Takeuchi, M., Inoue, N., Strickland, T. W., Kubata, M., Wada, M., Shimizu, R., Hoshi, S., Kozutsumi, H., Takasaki, S. and Kobata, A. 1989. Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA. 86:7819-7822 |
10 | Yamaguchi, K., Akai, K., Kawanishi, G., Ueda, M., Masuda, S. and Sasaki, R. 1991. Effects of site-directed removal of N-glycosylation sites in human erythropoietin on its production and biological properties. J. BioI. Chem. 266: 20434-20439 |
11 | Fibi, M. R., Hermentin, P., Pauly, J. U., Lauffer, Land Zettlmeissl, G. 1995. N- and O-glycosylation muteins of recombinant human erythropoietin secreted from BHK-21 cells. Blood 85:1229-1236 |
12 | Cointe, D., Beliard, R., Jorieux, S., Leroy, Y., Glacet, A., Verbert, A., Bourel, D. and Chirat, F. 2000. Unuaual N-glycosylation of a recombinant human erythropoietin expressed in a human Iympholastoid cell line does not alter its biological properties. Glycobiology 10:511-519 |
13 | Bill, R. M., Flitsch, S. L. and Bicknell, R. 1997. COS-1 cell expression and one-step affinity protein purification and activity of epitopetagged human erythropoietin and of site-directed mutants. Biochim. Biophys. Acta. 1340:13-20 |
14 | Wasley, L. C., Timony, G., Murtha, P., Stoudemire, J., Dorner, A. J., Caro, J., Krieger, M. and Kaufman, R. J. 1991. The importance of N- and O-linked oligosaccharides for the biosynthesis and in vitro and in vivo biologic activities of erythropoietin. Blood 77:2624-32 PUBMED |
15 | Fukuda, M. N., Sasaki, H., Lopez, L. and Fukuda, M. 1989. Survival of recombinant erythropoietin in the circulation: the role of carbohydrates. Blood 73:84-89 |
16 | Goldwasser, E. and Kung, C. K. 1968. Progress in the purification of erythropoietin. Ann. N. Y. Acad. Sci. 29:49-53 |
17 | Wen, D., Boissel, J. P., Tracy, T. E., Gruninger, R. H., Mulcahy, L. S., Czelusniak, J., Goodman, M. and Bunn, H. F. 1993. Erythropoietin structure function relationships: high degree of sequence homology among mammals. Blood 82:1507-1516 |
18 | Nagao, M., Suga, H., Okano, M., Masuda, S., Narita, H., Ikura, K. and Sasaki, R. 1992. Nucleotide Sequence of rat erythropoietin. Biochim. Biophys. Acta. 1171:99-102 |
19 | Ureana, P. 2002. Treatment of anemia in chronic renal failure by a long-activing activator of erythropoiesis. Presse. Med. 23;31:505-514 |
20 | Narhi, L. O., Arakawa, T., Aoki, K. H., Elmore, R., Rohde, M. F., Boone, T. and Strickland, T. W. 1991. The effect of carbohydrate on the structure and stability of erythropoietin. J. BioI. Chem. 266:23022-23026 |
21 | McDonald, J. D., Lin, F. K. and Goldwasser, E. 1986. Cloning, Sequencing and Evolutionary Analysis of the Mouse Erythropoietin Gene. Mol. Cell BioI. 6:842-848 DOI |
22 | Macdougall, I. C., Gray, S. J., Elston, O., Breen, C., Jenkins, B., Browne, J. and Egrie, J. 1999. Pharmacokinetics of Novel Erythropoiesis Stimulating Protein Compared with Epoetin Alfa in Dialysis Patients. J. Am. Soc. Mephol. 10: 2392-2395 |
23 | Sasaki, H., Bothner, B., Dell, A. and Fukuda, M. 1987. Carbohydrate structure of erythro-poiettn expressed in Chinese hamster ovary cells by a human erythropoietin cDNA. J. BioI. Chem. 262:12059-12076 |
24 | Dube, S., Fisher, J. W. and Powell, J. S. 1988. Glycosylation at specific sites of erythropoietin is essential fer biosynthesis, Secretion, and biological function. J. BioI. Chem. 263:17516-17521 |
25 | Egrie, J. C. and Browne, J K. 2001. Development and characterization of novel erythropoiesis stimulating protein (NESP). Nephrol Dial Transplant 3:3-13 |