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Effects of Growth Factors and Hexoses on In Vitro Development in Porcine Embryos  

윤선영 (강원대학교 동물자원과학대학)
이상영 (경상남도 첨단양돈연구소 생명공학과)
정희태 (강원대학교 동물자원과학대학)
양부근 (강원대학교 동물자원과학대학)
김정익 (강원대학교 동물자원과학대학)
박춘근 (강원대학교 동물자원과학대학)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.3, 2003 , pp. 249-258 More about this Journal
Abstract
The aim of this study was to investigate the effects of different hexoses in medium with IGF-I and/or EGF on in vitro development of porcine embryos. In first experiment, when the embryos were cultured in medium with concentrations of 5 ng/ml IGF-I and 10 ng/ml EGF, the morula and blastocyst rates were higher than in another culture conditions (P<0.05). In second experiment, the effect of IGF-I in medium with different hexoses during the embryo culture was examined. The higher cleavage rates were obtained in medium with glucose and IGF-I (P<0.05). However, the higher proportion of embryos developed to morula and blastocyst stages in medium with IGF-I were obtained than in medium without IGF-I in medium with glucose, mannose and galactose. In third experiment, the effect of EGF on in vitro development of porcine embryos in medium with different hexoses were examined. In the culture medium was supplemented with glucose, the higher proportions (P<0.05) of embryos developed to molura and blastocyst stages were obtained in medium with that than without EGF. However, the proportions of embryos developed to blastocyst stage were not significantly different between with and without of EGF in medium with different hexoses. In firth experiment, the effects of presence of IGF-I and EGF in medium with different hexoses on in vitro development of porcine embryos were examined. When culture medium was supplemented with IGF-I and EGF, the higher proportions of oocytes cleaved and developed to 8-cell stage were obtained in medium with glucose than mannose, galactose and fructose (P<0.05). These results show that glucose and growth factors, supported the development in vitro of porcine embryos, especially with greater embryo cleavage and development in medium with glucose added to media with IGF-I and/or EGF.
Keywords
EGF; Hexoses; In vitro development; IGF-I; Porcine embryo;
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