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Transfer and Expression of the Recombinant hFSH Gene in CHO Cells and Transgenic Chickens using Retrovirus Vector System  

권모선 (대구가톨릭대학교 의과대학 생리학교실)
구본철 (대구가톨릭대학교 의과대학 생리학교실)
심호섭 (단국대학교 의과대학 생리학교실)
박창식 (충남대학교 동물자원학부, 충남대학교 형질전환 복제돼지 연구센터)
이성호 (공주대학교 영상보건대학, 충남대학교 형질전환 복제돼지 연구센터)
김태완 (충남대학교 형질전환 복제돼지 연구센터)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.3, 2003 , pp. 197-206 More about this Journal
Abstract
hFSH (human follicle stimulating hormone) is heterodimer consisting of $\alpha$ and $\beta$ subunits. Since assembly of the both subunits in the cell is often the rate-limiting step in production of functional hormone, single-chain hormones have been engineered by genetically linking two different cDNA fragments with a linker sequence. Using retrovirus vector system, the resulting recombinant hFSH gene was transferred in CHO cells and chicken embryos, and the expression of the gene was investigated. In CHO cells, protein synthesis from the single-chain FSH gene was 17 fold higher than that from the heterodimeric counterpart. In the study of transgenic chickens, ten of the eleven chicks hatched from 62 embryos manupulated with recombinant retrovirus stock was determined to carry transgenic genes. RT-PCR analyses confirmed transcription of the single-chain FSH gene, however, no recombinant FSH was detected from the blood samples.
Keywords
hFSH gene; CTP; CHO cell; Transgenic chicken; RT-PCR;
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