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Study of Efficient Production of Cloned Embryos in Porcine II. Establishment of Conditional Enucleation  

위갑인 (전남대학교 농업생명과학대학 동물자원학부, 농업과학기술연구소)
강만종 (전남대학교 농업생명과학대학 동물자원학부, 농업과학기술연구소)
문승주 (전남대학교 동물자원학부)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.3, 2003 , pp. 187-195 More about this Journal
Abstract
This study was carried out to improve of enucleation efficiency on porcine recipient oocytes preactivated. In ethanol or $Ca^{2+}$ ionophore, effect of repeating and combinational activation with 6-DMAP or cycloheximide compared with alone activated treatment. Recipient oocytes's activation by $Ca^{2+}$ ionophore combined with 6-DMAP or cycloheximide were significantly higher than alone treatment(P<0.05). Between repeating and alone treatments were not significantly different. In ethanol, repeating treatment was significantly lower than alone(P<0.05), and combination treatments were not significantly different. On the basis of these results, efficiency of enucleation, electrical fusion and in vitro development compared preactivated with non-preactivated recipient oocytes. Enucleation and fusion rates of preactivated oocytes were improved significantly compared with non-preactivated oocytes(90.7%, 71.8 vs 77.8%, 61.1%; P<0.05). Behind the back, cleavage and in vitro development rates were significantly lower than non-preactivated oocytes(38.7%, 19.3% vs 68.8%, 30.6%; P<0.05).
Keywords
Enucleation; Recipient oocytes; Preactivation; Combinational activation;
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