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Effect of Oxygen Concentrations with Catalase on In Vitro Maturation of Porcine Follicular Oocytes and In Vitro Development of Porcine IVM/IVF Embryos  

한만희 (축산기술연구소)
이경본 (충남대학교 동물자원학부)
천행수 (충남대학교 동물자원학부)
박병권 (공주대학교 영상보건대학 특수동물학과)
이경광 (한국생명공학연구원)
이규승 (충남대학교 동물자원학부)
서길웅 (충남대학교 동물자원학부)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.2, 2003 , pp. 115-123 More about this Journal
Abstract
The present study was carried out to examine the effect of catalase (CAT) on in vitro maturation (IVM) of porcine follicular oocytes and oxygen concentration with CAT on in vitro development (IVD) of porcine IVM/IVF embryos. The results were summarized as follows - 1 . The rates of nuclear maturation, penetrated oocytes, pronucleus formation rates, polyspermic oocytes and mean numbers of the penetrated sperm were significantly lower in oocytes matured with 100, 500 and 1,000 units/ml CAT than those of central groups (P>0.05). 2. The rates of blastocyst formation and total cell numbers of blastocyst at day 7 after in vitro fertilization were significantly lower in CAT treatment groups than those of the central groups (P>0.05). 3. There were not significant difference in the blastocyst development and total cell numbers of blastocyst on in vitro culture of NCSU-23 media with 0, 100, 500 and 1000 units/ml CAT under the 5% and 20% $O_2$ concentrations. These results suggested that the addition of CAT was not helpful for porcine oocyte maturation and further development, also the rates of blastocyst formation and total cell numbers of blastocyst at day 7 of porcine IVM/IVF embryos were not significantly different in the NCSU-23 culture medium under the 5% and 20% $O_2$ concentrations.
Keywords
Porcine embryos; IVM; IVF; Catalase; $O_2$ concentration;
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