Browse > Article

Generation of Transgenic Mice with Overexpression of Mouse Resistin  

Lee, H. T. (College of Animal Science, Konkuk university)
J. R. Chun. (College of Animal Science, Konkuk university)
K. S. Chung (College of Animal Science, Konkuk university)
Publication Information
Korean Journal of Animal Reproduction / v.26, no.4, 2002 , pp. 321-328 More about this Journal
Abstract
The hormone resistin is associated with type II diabetes mellitus in rodent model. Resistin impairs glucose tolerance and insulin action. A new class of anti-diabetic drugs were called thiazolidinediones (TZDs) downreguates a resistin. Resistin gene expression is induced during adipocyte differentiation and resistin polypeptide is secreted by adipocytes. But, the correlation between increased adiposity and resistin remains unknown. The objectives of this study was to clone a mouse resistin CDNA and to generate transgenic mice overexpressing mouse resistin gene. The pCMV-mus/resistin gene was prepared from previous recombinant pTargeT$^{TM}$-mus/resistin by digestion of Bgl II, and has used for microin- jection into pronuclei of one cell embryos. Mouse resistin expression was detected in transgenic F$_1$mice by RT-PCR. The transgenic mouse with resistin gene expression has heavier body weight which was measured higher level of plasma glucose than that of normal mouse. And in diet-induced experiments, in fasting group, resistin expression was higher than that of re-feeding group. This result demonstrates that the resistin gene overexpressing mice may be became to obesity and be useful as an animal disease model to be diabetes caused by insulin resistance of resistin.n.
Keywords
Resistin; Obesity; Diet-induced; Transgenic;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Banerjee, R. R. et al. 2001. Dimerization of resistin and resistin-like molecules is determined by a single cyste ine. J. BioI. Chem. MI03109200.
2 Claire, M. S. 2001. A family of tissue-specific resistin-like molecules. PNAS 98:502-506   DOI   ScienceOn
3 Hartman, B. H. et al. 2002. Mechanisms regulating adipocyte expression of resistin. J. Bio. Chem. M201451200.
4 Kahn, B. B. et al. 2000. Obesity and insulin resistance. J. Clin. Invest., 106:467-472   DOI   ScienceOn
5 Shao, D. et al. 1997. Peroxisome proliferator activated receptor ${\gamma}$, CAAT/enhancer-binding protein ${\alpha}$, and cell cycle status regulate the commitment to adipocyte differentiation. J. BioI. Chem., 272:21473-21478
6 Vernon, R. G. et al. 2001. Signal of adiposity. Dom. Animal Endocrinol., 21:197-214   DOI   ScienceOn
7 Steppan, C. M. et al. 2001. The hormone resistin links obesity to diabetes. Nature, 409:307-312   DOI   ScienceOn
8 Kim, K. H. et al. 2001. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation. J. BioI. Chem., 276:11252-11256
9 Engert, J. C. et al. 2002. 5' flanking variants of resistin are associated with obesity. Diabetes, 51: 1629-1634
10 Lay, S. L. et al. 2001 Decreased resistin expression in mice with different sensitivities to a high-fat diet. Biochem. Biophys. Res. Commun., 289:564-567
11 McTernan, C. L. et al. 2002. Resistin, central obesity, and type 2 diabetes. Reserch Letters, 359:46-47
12 Hotamisligil, G. et. al. 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science, 259:87-91
13 Juan, C. C. et al. 2001. Suppressed gene expression of adipocyte resistin in an insulin -resistant rat model probably by elevated free fatty acids. Biochem. Biophys. Res. Commun., 289:1328-1333
14 Morash, B. A. et al. 2002. Resistin expression and regulation in mouse pituitary. REBS Letters, 526:26-30
15 Fasshaurer, M. et al. 2001. Tumor necrosis factor a is negative regulator of resistin gene expression and secretion in 3T3-LI adipocytes. Biochem. Biophys. Res. Commun., 288:1027-1031
16 Kahn, C. R. et al. 1996. A Genetics of non-insulin-dependent type 2 diabetes mellitus. Annu. Rev. Med., 47:509-531   DOI   ScienceOn
17 Way, J. M. et al. 2001. Adipose tissue resistin xpression is severely suppressed in obesity. J. Bio. Chem., 276:25651-25653 (Received September 10, 2002; Accepted November 10, 2002)
18 Osawa, H. et al. 2002. Systematic search for single nucleotide polymorphisms in the resistin gene. Diabetes, 51:863-866   DOI   ScienceOn
19 Holocomb, I. N. et al. 2001. FIZZI, a novel cystein-rich secreted protein associated with pulmonary inflammation, defines a new gene family. EMBO J., 19:4046-4055
20 Claire, M. S. 2001. A family of tissue-specific resistin-like molecules. PNAS 98:502-506   DOI   ScienceOn
21 Veerababu, G. et al. 2000. Overexpression of glutamine: fructose-6-phosphate amidotransferase in the liver of transgenic mice results in enhanced glycogen storage, hyperlipidemia, obesity, and impaired glucose tolerance. Diabetes, 49:2070-2078
22 Blagoev, B. et al. 2002. Inhibition of adipocyte differentiation by resistin like molecule a(RELM a): Biochemical characterization of its oligomeric nature. 1. BioI. Chern
23 Auwerx, J. et al. 1996. Transcription, adipocyte differentiation, and obesity. J. Mol. Med., 74: 347-352
24 Ling, C. et al. 2001. Increased resistin expression in the adipose tissue of male prolactin transgenic mice and male mice with elevated androgen levels. FEBS Letters, 507:147-150   DOI   ScienceOn
25 Stevens, J. et al. 1981. Insulin resistance in adipocytes from fed and fasted obese rats: dissociation of two insulin actions. Mol. Cell. Biochem., 37:177-183   DOI   ScienceOn