Browse > Article

In Vitro Maturation, Fertilization and Development of Mouse Oocytes Derived from In Vitro Grown Preantyal Follicles  

Kim, D.H. (Medical science Institute, Eulji General Hospital)
Lee, H.J. (Department of Physiology, Eulji University School of Medicine)
K.S. Chung (Animal Resource Research Center, Konkuk University)
Lee, H.T. (Animal Resource Research Center, Konkuk University)
Publication Information
Korean Journal of Animal Reproduction / v.26, no.2, 2002 , pp. 133-142 More about this Journal
Abstract
The objective of this study was to examine maturation, fertilization and developmental rate of the in vitro-grown mouse oocytes, and to compare these results with those of oocytes grown and matured in vivo. The preantral follicles isolated from 12-day-old mice were cultured on Transwell-COL membrane inserts. After in vitro growth and maturation, 72.5 % of oocytes grown in vitro produced polar body which can be comparable to in vivo growth (70.5 %). However, the mean oocyte diameter of the in vitro group (69.6$\pm$2.1$\mu$m) was smaller than that of the in vivo group (73.3$\pm$3.0$\mu$m). The fertilization rate was significantly lower (p<0.05) in the in vitro group (76.5%) than in the in vivo group (90.2%), however, there was no difference in the percentage of monospermic and polyspermic oocytes between two groups. The capacities of in vitro grown ova to cleave and develop to blastocyst were (57.8 and 14.4%, respectively) significantly lower (p<0.001) than those of the in vivo counterpart (84.4 and 56.6%, respectively). Moreover, the mean number of cells per blastocyst was significantly lower (p<0.05) in the in vitro group (39.0$\pm$10.8) than in the in vivo group (60.5$\pm$12.5). Live young were produced from transferred 2-cell embryos derived from in vitro-grown and matured oocytes. In conclusion, the results show that in vitro-grown oocytes did not achieve the developmental capacity of in vitro-grown oocytes.
Keywords
Mouse; In vitro growth; Preantral follic1e; Live young;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Tilly, J. L. and Tilly, K. I. 1995. Inhibitors of oxidative stress mimic the ability of follicle stimulating hormone to suppress apoptosis in cultured rat ovarian follicles. Endocrinology, 136:242-252   DOI   PUBMED   ScienceOn
2 Eppig, J. J. and Wigglesworth, K. 1995. Factors affectings the developmental competence of mouse oocytes grown in vitro; oxygen concentration. Mol. Reprod. Dev., 42:447-456   DOI   ScienceOn
3 Hirao, Y., Nagai, T., Kubo, M., Miyano, T., Miyake, M. and Kato, S. 1994. In vitro growth and maturation of pig oocytes. J. Reprod. Fertil., 100:333-339   DOI   PUBMED   ScienceOn
4 Nayudu, P. L. and Osborn, S. M. 1992. Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J. Reprod. Fertil., 95:349-362   DOI   PUBMED   ScienceOn
5 Smitz, J., Cortvrintz, A. and Van Steirteghem, A. 1996. Normal oxygen atmosphere is essential for the solitary long-term culture of early preantral mouse follicles. Mol. Reprod. Dev., 45:466-475   DOI   ScienceOn
6 Vanderhyden, B. C. and Armstrong, D. T. 1989. Role of cumulus cells and serum in the in vitro maturation, fertilization and subsequent development of rat oocytes. Biol. Reprod., 40:720-728   DOI   ScienceOn
7 Eppig, J. J. and Schroeder, A. C. 1989. Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation and fertilization in vitro. Biol Reprod., 41:268-276   DOI   ScienceOn
8 Cha, K. Y., Choi, D. H., Koo, J. J., Han, S. Y., Ko, J. J., and Yoon, T. K. 1991. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a .donor oocyte program. Fertil. Steril., 55:109-113   DOI   PUBMED
9 Yong, E. L., Baird, R., Yates, L. E., Reichert, L. E. Jr. and Hillier, S. G. 1992. Hormonal regulation of the growth and steroidogenic function of human granulosa cells. J. Clin. Endocrinol. Metab., 74:842-849   DOI   PUBMED
10 Eppig, J. J. and Down, S. M. 1987. The effect of hypoxanthine on mouse oocyte growth and development in vitro; maintenance of meiotic arrest and gonadotrophin-induced oocyte maturation. Dev. Biol., 119:313-321   DOI   ScienceOn
11 Mattioli, M., Bacci, M. L., Galeati, G. and Seren, E. 1989. Developmental competence of pig oocytes matured and fertilized in vitro. Theriogenology, 31:1201-1207
12 Hirao, Y., Miyano, T. and Kato, S. 1990. Fertilization of in vitro grown mouse oocytes. Theriogenology, 34:1071-1077
13 Roy, S. K. and Treacy, B. J. 1993. Isolation and long-term culture of human preantral follicles. Fertil. Steril., 59:783-790   DOI   PUBMED
14 Hartshorne, G. M., Sargent, I. L. and Barlow, D. H. 1994. Meiotic progression of mouse oocytes throughout follicle growth and ovulation in vitro. Human. Reprod., 9:352-359   DOI
15 Staigmiller, R. B. and Moor, R. M. 1984. Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle. Gamete Research, 9:221-229   DOI
16 Torrance, C., Telfer, E. and Gosden, R. G. 1989. Quantitative study of the development of isolated mouse pre-antral follicles in collagen gel culture. J. Reprod. Fertii., 87:367-374   DOI   ScienceOn
17 Goto, K, Kajihara, Y., Kosaka, S., Koba, M., Nakanishi, Y. and Ogawa, K. 1988. Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vivo matured follicular oocytes. J. Reprod. Fertil., 83:753-758   DOI   PUBMED   ScienceOn
18 Kim, D-H., Chi, H. J., Kang, H. G., Han, S. W., Lee, H. T., Chung, K. S. and Lee, H. J. 1999. Effects of gonadotrophins on in vitro growth and maturation of mouse preantral follicles. Kor. J. Anim. Reprod., 23:53-61
19 Schroeder, A. C. and Eppig, J. J. 1984. The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev. Biol., 102:493-497   DOI   ScienceOn
20 Cortvrindt, R., Smitz, J. and Van Steirteghem, A. C. 1996. In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Human. Reprod., 11 :2656-2666   DOI   PUBMED   ScienceOn
21 Qvist, R., Blackwell, L. F., Bourne, H. and Brown. J. B. 1990. Development of mouse ovarian follicles from primary to preovulatory stages in vitro. J. Reprod. Fertil., 89:169-180   DOI   PUBMED   ScienceOn
22 Figueiredo, J. R, Hulshof, S. C. J., Van den Hurk, R., Bevers, M. M., Nusgens, B. and Beckers, J. F. 1993. Development of a combined new mechanical and enzymatic method for the isolationof intact preantral follicles from fetal, calf and adult bovine ovaries. Theriogenology, 40:789799
23 Spears, N., Boland, N. I., Murray, A. A. and Gosden, R. G. 1994. Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile. Human. Reprod., 9:527-532   DOI
24 Eppig, J. J. and O'Brien, M. J. 1996. Development in vitro of mouse oocytes from primordial follicles. Biol. Reprod., 54:197-207   DOI   ScienceOn
25 De La Fuente, R., OBrien, M. J. and Eppig, J. J. 1999. Epidermal growth factor enhances preimplantation developmental competence of maturing mouse oocytes. Human. Reprod., 14:30603068
26 Gosden, R. G. and Telfer, E. 1987. Numbers of follicles and oocytes in mammalian ovaries and their allometric relationships. J. Zool., 211:169 -175
27 Carroll, J., Whittingham, D. G. and Wood, M. J. 1991. Effect of dibutyryl cyclic adenosin monophosphate on granulosa cell proliferation, oocyte growth and meiotic maturation in isolated mouse primary ovarian follicles cultured in collagen gels. J. Reprod. Fertil., 92:197-207   DOI   PUBMED   ScienceOn
28 Cortvrindt, R., Hu, Y. and Smitz, J. 1998. Recombinant luteinizing hormone as a survival and differentiation factor increases oocyte maturation in recombinant follicle stimulating hormone-supplemented mouse preantral follicle culture. Human. Reprod., 13:1292-1302
29 Boland, N. I., Humpherson, P. G., Leese, H. J. and Gosden, R. G. 1993. Pattern of lactate production and steroidogenesis during growth and maturation of mouse ovarian follicles in vitro. Biol. Reprod., 48:798-806   DOI   ScienceOn
30 Eppig, J. J., Schroeder, A. C., van de Sandt, J. J. M., Ziomek, C. A. and avister, B. D. 1990. Developmental capacity of mouse oocytes that grow and ature in culture; the effect of modification of the protocol. Theriogenology,33:89-100   DOI   ScienceOn
31 Boland, N. I. and Gosden, R. G. 1994. Effects of epidermal growth factor on the growth and differentiation of cultured mouse ovarian follicles. J. Reprod. Fertil., 101:369-374   DOI   PUBMED   ScienceOn
32 Smitz, J., Cortvrintz, A. and Hu, Y. 1998. Epidermal growth factor combined with recombinant human chorionic gonadotrophin improves meiotic progression in mouse follicle-enclosed oocyte culture. Human. Reprod., 13:664-669   DOI   ScienceOn