Browse > Article
http://dx.doi.org/10.5635/ASED.2020.36.1.025

COI DNA Barcoding for Sterkiella multicirrata (Ciliophora: Oxytrichidae) from South Korea  

Kim, Kang-San (Department of Biological Sciences, Inha University)
Ji, Su-Jung (Department of Biological Sciences, Inha University)
Min, Gi-Sik (Department of Biological Sciences, Inha University)
Publication Information
Animal Systematics, Evolution and Diversity / v.36, no.1, 2020 , pp. 7-9 More about this Journal
Abstract
In the present study, the first mitochondrial cytochrome c oxidase subunit I gene (COI) sequence of Sterkiella multicirrata Li et al., 2018 is presented. To begin with, this species has been also morphologically recorded from South Korea, and this study was performed using genomic DNA of the Korean population. The newly obtained COI sequences of S. multicirrata were identical. And the inter-specific variation between S. multicirrata and S. histriomuscorum was noted at 14.3%. These values correspond well with the results of previous studies. However, because there are very few available COI sequences of stichotrichian in GenBank, it is concluded that continuous accumulation of data is needed for further study.
Keywords
Ciliophora; COI; DNA barcode; Stichotrichia; Stylonychinae;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Chen X, Gao F, Al-Farraj SA, Al-Rasheid KA, Xu K, Song W, 2015. Morphology and morphogenesis of a novel mangrove ciliate, Sterkiella subtropica sp. nov. (Protozoa, Ciliophora, Hypotrichia), with phylogenetic analyses based on small-subunit rDNA sequence data. International Journal of Systematic and Evolutionary Microbiology, 65:2292-2303. https://doi.org/10.1099/ijs.0.000253   DOI
2 Foissner W, 2016. Terrestrial and semiterrestrial ciliates (Protozoa, Ciliophora) from Venezuela and Galápagos. Denisia, 35:1-912.
3 Berger H, 1999. Monograph of the Oxytrichidae (Ciliophora, Hypotrichia). Monographiae Biologicae, Vol. 78. Springer, Dordrecht, pp. 1-1080.
4 Chen L, Zhao X, Shao C, Miao M, Clamp JC, 2017. Morphology and phylogeny of two new ciliates, Sterkiella sinica sp. nov. and Rubrioxytricha tsinlingensis sp. nov. (Protozoa, Ciliophora, Hypotrichia) from north-west China. Systematics and Biodiversity, 15:131-142. https://doi.org/10.1080/14772000.2016.1219426   DOI
5 Hall TA, 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41:95-98.
6 Jung JH, Park KM, Min GS, 2014. Morphology and molecular phylogeny of Pseudouroleptus jejuensis nov spec., a new soil ciliate (Ciliophora, Spirotrichea) from South Korea. Acta Protozoologica, 53:195-206. https://doi.org/10.4467/16890027AP.14.016.1597
7 Kim JH, Moon JH, Jung JH, 2018. New records of 24 ciliate species (Protozoa, Ciliophora) collected in South Korea. Journal of Species Research, 7:291-314. https://doi.org/10.12651/JSR.2018.7.4.291   DOI
8 Kim KS, Min GS, 2018. Brief morphological description of stichotrichid ciliates (Ciliophora: Stichotrichia) from Korea. Journal of Species Research, 7:323-332. https://doi.org/10.12651/JSR.2018.7.4.323   DOI
9 Kumar S, Kamra K, Bharti D, La Terza A, Sehgal N, Warren A, Sapra GR, 2015. Morphology, morphogenesis, and molecular phylogeny of Sterkiella tetracirrata n. sp. (Ciliophora, Oxytrichidae), from the Silent Valley National Park, India. European Journal of Protistology, 51:86-97. https://doi.org/10.1016/j.ejop.2014.12.002   DOI
10 Li F, Li Y, Luo D, Miao M, Shao C, 2018. Morphology, morphogenesis, and molecular phylogeny of a new soil ciliate, Sterkiella multicirrata sp. nov. (Ciliophora, Hypotrichia) from China. Journal of Eukaryotic Microbiology, 65:627-636. https://doi.org/10.1111/jeu.12508   DOI
11 Lynn DH, 2008. The ciliated protozoa: characterization, classification, and guide to the literature. 3rd ed. Springer, Dordrecht, pp. 1-604.
12 Park MH, Jung JH, Jo E, Park KM, Baek YS, Kim SJ, Min GS, 2019. Utility of mitochondrial CO1 sequences for species discrimination of Spirotrichea ciliates (Protozoa, Ciliophora). Mitochondrial DNA Part A, DNA Mapping, Sequencing, and Analysis, 30:148-155. https://doi.org/10.1080/24701394.2018.1464563
13 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28:2731-2739. https://doi.org/10.1093/molbev/msr121   DOI
14 Schmidt SL, Bernhard D, Schlegel M, Foissner W, 2007. Phylogeny of the Stichotrichia (Ciliophora; Spirotrichea) reconstructed with nuclear small subunit rRNA gene sequences: discrepancies and accordances with morphological data. Journal of Eukaryotic Microbiology, 54:201-209. https://doi.org/10.1111/j.1550-7408.2007.00250.x   DOI
15 Jung JH, Park MH, Kim SY, Choi JM, Min GS, Kim YO, 2017. Checklist of Korean ciliates (Protozoa: Ciliophora). Journal of Species Research, 5:241-257. https://doi.org/10.12651/JSR.2017.6.3.241   DOI
16 Struder-Kypke MC, Lynn DH, 2010. Comparative analysis of the mitochondrial cytochrome c oxidase subunit I (COI) gene in ciliates (Alveolata, Ciliophora) and evaluation of its suitability as a biodiversity marker. Systematics and Biodiversity, 8:131-148. https://doi.org/10.1080/14772000903507744   DOI
17 Yi Z, Chen Z, Warren A, Roberts D, Al-Rasheid KAS, Miao M, Gao S, Shao C, Song W, 2008. Molecular phylogeny of Pseudokeronopsis (Protozoa, Ciliophora, Urostylida), with reconsideration of three closely related species at inter- and intra-specific levels inferred from the small subunit ribosomal RNA gene and the ITS1-5.8S-ITS2 region sequences. Journal of Zoology, 275:268-275. https://doi.org/10.1111/j.1469-7998.2008.00438.x   DOI
18 Zhao Y, Gentekaki E, Yi Z, Lin X, 2013. Genetic differentiation of the mitochondrial cytochrome c subunit I gene in genus Paramecium (Protista, Ciliophora). PLoS ONE, 8:e77044. https://doi.org/10.1371/journal.pone.0077044   DOI