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http://dx.doi.org/10.5635/ASED.2015.31.3.182

Novel Discovery of Two Heterotrichid Ciliates, Climacostomum virens and Fabrea salina (Ciliophora: Heterotrichea: Heterotrichida) in Korea  

Kim, Ji Hye (Department of Biological Science, College of Natural Sciences, University of Ulsan)
Shin, Mann Kyoon (Department of Biological Science, College of Natural Sciences, University of Ulsan)
Publication Information
Animal Systematics, Evolution and Diversity / v.31, no.3, 2015 , pp. 182-190 More about this Journal
Abstract
Two heterotrichid ciliates, Climacostomum virens (Ehrenberg, 1838) Stein, 1859 from brackish water and freshwater, and Fabrea salina Henneguy, 1890 from a solar saltern, were collected in Korea. They are novelly investigated in Korea by means of live observation, protargol staining and nuclear small subunit (SSU) rRNA gene sequencing. Climacostomum virens is characterized by pouch-like body shape, body length of $200-370{\mu}m$ in vivo, conspicuous cytopharyngeal tube, macronuclei ribbon-like shape, and one to four in number, with or without symbiont algae in cytoplasm, 34-66 somatic kineties, 67-113 adoral zone of membranelles, 8-42 peristomial kineties, 24-37 apical membranelles. SSU rDNA sequence size is 1,591 bp and GC contents 48.52%. Fabrea salina is also characterized by scoop-like body shape with proboscis, body length of $190-240{\mu}m$ in vivo, one to two rod-shaped macronuclei, oval micronuclei, grayish green cortical granules, 104-186 somatic kineties, 4-8 preoral kineties, 7-19 peristomial kineties and fragmented paroral membrane. SSU rDNA sequence size is 1,598 bp and GC contents 47.50%.
Keywords
brackish water; Climacostomum; Fabrea; freshwater; heterotrichs; Korea; morphology; solar saltern;
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1 Augustin H, Foissner W, 1992. Morphologie und Okologie einiger Ciliaten (Progozoa: Ciliophora) aus dem Belebtschlamm. Archiv fur Protistenkunde, 141:243-283. http://dx.doi.org/10.1016/S0003-9365(11)80057-6   DOI
2 Chen X, Song W, Al-Rasheid KAS, Warren A, Long H, Shao C, Al-Farraj SA, Gong J, Hu X, 2007. The morphology of three marine heterotrichous ciliates, Condylostentor auriculatus (Kahl, 1932) Jankowski, 1978, Condylostoma minutum Bullington, 1940 and C. spatiosum Ozaki & Yagiu in Yagiu, 1944 (Ciliophora: Heterotrichida). Acta Protozoologica, 46:289-309.
3 Chen XR, Ma HG, Zhu MZ, Hu XZ, 2011. Morphological studies on seven heterotrichid ciliates collected from the coastal waters of Qingdao. Acta Hydrobiologica Sinica, 35:920-928 (in Chinese with English summary).
4 Dragesco J, Dragesco-Kerneis A, 1986. Cilies libres de l'Afrique intertropicale. Ingroduction a la connaissance et a l'etude des cilies. Faune Tropicale, 26:1-559.
5 Dubochet CF, Peck RK, De Haller G, 1979. Morphogenesis in the heterotrich ciliate Climacostomum virens. I Oral development during cell division. Journal of Protozoology, 26:218-226. http://dx.doi.org/10.1111/j.1550-7408.1979.tb02764.x   DOI
6 Ehrenberg CG, 1838. Die Infusionsthierchen als Vollkommene Organismen. Ein Blick in das tiefere organische Leben der Naur. Verlag von Leopold Voss, Leipzig, pp. 1-548.
7 Foissner W, 1991. Basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa. European Journal of Protistology, 27:313-330. http://dx.doi.org/10.1016/S0932-4739(11)80248-8   DOI
8 Foissner W, Berger H, Kohmann H, 1992. Taxonomische und okologische revision der ciliaten des saprobiensystems -band II. Informationsberichte des Bayerischen Landesamtes fur Wasserwirtschaft, 5/92:394-399.
9 Hammerschmidt B, Schlegel M, Lynn DH, Leipe DD, Sogin ML, Raikov IB, 1996. Insights into the evolution of nuclear dualism in the ciliates revealed by phylogenetic analysis of rRNA sequences. Journal of Eukaryotic Microbiology, 43:225-230. http://dx.doi.org/10.1111/j.1550-7408.1996.tb01396.x   DOI   ScienceOn
10 Henneguy LF, 1890. Sur un Infusoire heterotriche Fabrea salina nov. sp. Annales de Micrographie, 3:118-135.
11 Kahl A, 1932. Urtiere oder Protozoa. I: Wimpertiere oder Ciliata (Infusoria) 3. Spirotricha. Die Tierwelt Deutschlands, 25:399-650.
12 Lynn D, 2008. The ciliated protozoa. Characterization, classification, and guide to the literature. 3rd ed. Springer, Dordrecht, pp. 1-605.
13 Repak AJ, 1972. A redesription of Climacostomum virens (Ehrenberg) Stein and proposal of a new heterotrich ciliate Family, Climacostomidae Fam. n. Journal of Protozoology, 19:417-427. http://dx.doi.org/10.1111/j.1550-7408.1972.tb03496.x   DOI
14 Medlin L, Elwood HJ, Stickel S, Sogin ML, 1988. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71:491-499. http://dx.doi.org/10.1016/0378-1119(88)90066-2   DOI   ScienceOn
15 Miao M, Song W, Clamp JC, Al-Rasheid KAS, Al-Khedhairy AA, Al-Arifi S, 2009. Further consideration of the phylogeny of some "traditional" heterotrichs (Protista, Ciliophora) of uncertain affinities, based on new sequences of the small subunit rRNA gene. Journal of Eukaryotic Microbiology, 56:244-250. http://dx.doi.org/10.1111/j.1550-7408.2009.00391.x   DOI   ScienceOn
16 Peck R, Pelvat B, Bolivar I, De Haller G, 1975. Light and electron microscopic observations on the heterotrich ciliate Climacostomum virens. Journal of Protozoology, 22:368-385. http://dx.doi.org/10.1111/j.1550-7408.1975.tb05187.x   DOI
17 Shao C, Song W, Hu X, Ma H, Zhu M, Wang M, 2006. Cell division and morphology of the marine ciliate, Condylostoma spatiosum Ozaki and Yagiu (Ciliophora, Heterotrichida) based on a Chinese population. European Journal of Protistology, 42:9-19. http://dx.doi.org/10.1016/j.ejop.2005.09.002   DOI   ScienceOn
18 Shazib SUA, Vd'acny P, Kim JH, Jang SW, Shin MK, 2014. Phylogenetic relationships of the ciliate class Heterotrichea (Protista, Ciliophora, Postciliodesmatophora) inferred from multiple molecular markers and multifaceted analysis strategy. Molecular Phylogenetics and Evolution, 78:118-135. http://dx.doi.org/10.1016/j.ympev.2014.05.012   DOI   ScienceOn
19 Song W, Warren A, Ji D, Wang M, Al-Rasheid KAS, 2003. New contributions to two heterotrichous ciliates, Folliculina simplex (Cons, 1917), Condylostoma curva Burkovsky, 1970 and one licnophorid, Licnophora lyngbycola Faure-Fremiet, 1937 (Protozoa, Ciliophora): descriptions of morphology and infraciliature. Journal of Eukaryotic Microbiology, 50:449-462. http://dx.doi.org/10.1111/j.1550-7408.2003.tb00271.x   DOI   ScienceOn
20 Song W, Packroff G, 1997. Taxonomy and morphology of marine ciliates from China with description of two new species, Strombidium globosaneum nov. spec. and S. platum nov. spec. (Protozoa, Ciliophora). Archive fur Protistenkunde, 147:331-360. http://dx.doi.org/10.1016/S0003-9365(97)80059-0   DOI   ScienceOn
21 Stein F, 1859. Der Organismus der Infusionsthiere nach eigenen Forschungen in systematischer Reihenfolge bearbeitet. I. Abtheilung. Allgemeiner Theil und Naturgeschichte der hypotrichen Infusionsthiere. W. Engelmann, Leipzig, pp. 1-206.
22 Stein F, 1867. Der Organismus der Infusionsthiere nach eigenen Forschungen in systematischer Reihenfolge bearbeitet. II. Abtheilung. 1) Darstellung der neuesten Forschungsergebnisse uber Bau, Fortpflanzung und Entwickelung der Infusionsthiere. 2) Naturgeschichte der heterotrichen Infusorien. W. Engelmann, Leipzig, pp. 1-355.
23 Wilbert N, 1975. Eine Verbesserte Technik der Protargolimpragnation fur Ciliaten. Microkosmos, 64:171-179.