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Cytogenetic Analysis in Korean Head and Neck Cancer Cell Lines： Comparative Genomic Hybridization(CGH) and Array-CGH  

Shin, You-Ree (Department of Otolaryngology, School of Medicine, Ewha Womans University)
Park, Soo-Yeun (Department of Anatomy, School of Medicine, Ewha Womans University)
Lee, Dong-Wook (Department of Otolaryngology, College of Medicine, Chungbuk National University)
Kim, Han-Su (Department of Otolaryngology, School of Medicine, Ewha Womans University)
Go, Young-Min (Deparment of Otolaryngology, Seoul Adventist Hospital)
Park, Hyun-Joo (Department of Otolaryngology, School of Medicine, Ewha Womans University)
Choung, Sung-Min (Department of Otolaryngology, School of Medicine, Ewha Womans University)
Publication Information
Korean Journal of Head & Neck Oncology / v.24, no.1, 2008 , pp. 33-42 More about this Journal
Abstract
Head and neck squamous cell carcinoma(HNSCC) is notorious for its poor outcome and increasing incidence. But, the studies of cytogenetic analysis in HNSCC are relatively rare, because of difficulties in culturing solid tumor cells and complexity in chromosomal DNA abberations associated with the lesions. The purpose of this study is to evaluate the location of chromosomal aberrations in Korean HNSCC cell lines (SNU-1041, 1066, and 1076) with comparative genomic hybridization(CGH) and array based CGH(array-CGH). Chromosomal gains of 3q23-q27, 5p13-p15.3, 7p21-pter, 8q11.2-q12, 8q21.1-qter, 9q22-q34, 16q22-q24, and 20q11.2-qter, as well as chromosomal losses on 3p10-p14 were found in all 3 SNU cell lines. Losses on 3p15- p23, 4q22-q27, 4q31.3-qter, 6q14-q15, 7q31-q34, 8p12-pter, 18q21-q23, and 21q11.2-q12 were observed in 2 of 3 cell lines. In array-CGH, many genes were altered including gains of PIK3CA, MYC, EVI1, MAD1L1 genes and losses of SERPIN genes. These aberrations of gene and chromosome coincide with other results of study, generally. These data about the patterns of chromosomal aberrations could be a basic step for understanding more detailed genetic events in the carcinogenesis and also provide information for diagosis and treatment in HNSCC.
Keywords
Genomic hybridization; Head and neck cancer; Squamous cell carcinoma; Cytogenetic analysis;
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