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The Effect of Transforming Growth Factor-${\beta}1$ on Expression of MMP 2 and MMP 9 Cell Lines  

Kwon Nam-Young (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine)
Kim Hyung-Jin (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine)
Woo Jeong-Su (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine)
Kwon Soon-Young (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine)
Jung Kwang-Yoon (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine)
Publication Information
Korean Journal of Head & Neck Oncology / v.18, no.2, 2002 , pp. 135-141 More about this Journal
Abstract
Backgrounds and Objectives: Metastasis is a complex multistep process that requires sequential interactions between the invasive cell and the extra-cellular matrix. Transforming growth factor-${\beta}1$ ($TGF-{\beta}1$) is a multifunctional regulator of cellular differentiation, motility and growth. Loss of sensitivity to the growth inhibitory effects by $TGF-{\beta}1$ plays important roles in neoplastic progression. The aim of this study was to investigate the role of $TGF-{\beta}1$ in the neoplastic invasion and metastasis through matrix metalloproteinase (MMP) of laryngeal cancer cell lines. Material and Methods: Two laryngeal cancer cell lines, SNU-899 and SNU-1076 were treated with recombinant $TGF-{\beta}1$, and the expression of MMP-2 and MMP-9 was immunohistochemically evaluated and gelatinase activity was studied by gelatin zymogram. Results: The cell growth inhibition was evident on 4th days after 1ng/ml and 10ng/ml $TGF-{\beta}1$ treatment. The expressions of MMP-2 and MMP-9, and their gelatinase activities were increased in dose-dependent manner. Conclusion: $TGF-{\beta}1$ treatment in laryngeal cancer cell lines induces the expression of MMP-2 and MMP-9, thus playing a role in the digestion of extracellular matrix gelatin.
Keywords
Laryngeal neoplasms; Metastasis; $TGF-{\beta}1$; MMP2; MMP9;
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