Browse > Article

Immunohistochemistry of Human Telomerase Reverse Transcriptase in Head and Neck Squamous Cell Carcinoma and Resection Margin  

Kim Yong-Bum (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Rho Kyung-Sup (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Hong Nam-Pyo (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Ahn Hwoe-Young (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Lee Yong-Sik (Department of Otolaryngology-Head and Neck Surgery, Korea Cancer Center Hospital)
Song Young-Ho (Department of Otolaryngology, College of Medicine, Kyung Hee University)
Publication Information
Korean Journal of Head & Neck Oncology / v.18, no.1, 2002 , pp. 18-22 More about this Journal
Abstract
Background and Objectives: The expression of telomerase, a ribonucleoprotein complex, has been detected in tissues from many human cancers, but not in the majority of normal tissues except germ cell. It is believed that the activation of telomerase is linked to celluar immortality and may playa role in tumorigenesis. Human telomerase reverse transcriptase (hTERT) has been identified as a putative catalytic subunit of human telomerase and its expression is closely correlated with telomease activity. We studied the expression of hTERT in head and neck squamous cell carcinoma (HNSCC) and resection margin by immunohistochemistry for hTERT and evaluate the correlation between hTERT expression and clinical data in HNSCC. Materials and Methods: We performed a immunohistochemistry in 17 cases of HNSCC and 10 cases of resection margins, histologically normal. The correlations between the hTERT expression and the clinical data in HNSCC were analyzed. Result: hTERT immunoreactivities were detected in 14 of 17 (82.4%) HNSCC, 1 of 10 (10%) resection margin. No correlation was observed between clinical data and hTERT expression in HNSCC. Conclusion: hTERT is activated in HNSCC and its expression is independent from clinical data of patients.
Keywords
Telomerase; Immunohistochemistry; hTERT; Squamous cell carcinoma;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Blackburn EH : Telomerase. Annu Rev Biochemi. 1992 ; 61 : 113-129   DOI   PUBMED   ScienceOn
2 Meyerson MC, Connter CM, Eaton EN, et al : hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell. 1997 ; 90 : 785-795   DOI   ScienceOn
3 Curran AJ, Denis KS, Irish J, Gullane PJ, MacMillan C, KamelReid S : Telomerase activity in oral squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 1998 ; 124 : 784-788   DOI   PUBMED
4 Hoaus S, Cavallo S, Bellascosa A, et al : Telomerase activity in human laryngeal squamous cell carcinoma. Clin. Cancer Res. 1996 ; 2 : 1895-1900
5 Brennan JS, Mao L, Hrnban R : Molecular assessment ofhisto-pathilogical staging in squamous-cell carcinoma ofhead andneck. N Engl J Med. 1995 ; 332 : 429-435   DOI   ScienceOn
6 FrancchiollaNS, Capaccio P, Carboni N, et al : Immunohistochemical and molecular analysis ofbax, bcl-2 and p53 genes in laryngeal squamous cell carcinomas. Anticancer Res. 1999 ; 19 : 1043-1051
7 Kim NW,Piatyszek MA, Prowse KR, et al : Specific association of human telomerase activity with immortal cells and cancer. Science. 1994 ; 226 : 2011-2015
8 Yasui W, Tahara E, Thara H, et al : Immunohistochemical detection of human telomerase reverse transcriptase in normal mucosa and precancerous lesions of the stomach. Jpn J Cancer Res. 1999 ; 909 : 589-595
9 Nakamura TM, Morin GB, Chapman KB, et al : Telomerase catalytic subunit homologs from fission yeast and human. Science. 1997 ; 277 : 955-959   DOI   PUBMED   ScienceOn
10 Kudo Y, Takata T, Ogawa I, Sato S, Nikai H : Expression ofp53 and p21CIP1/WAF1 proteins in oral epithelial dysplasia and squamous cell carcinomas. Oncol Rep. 1999 ; 6 : 539-545   PUBMED
11 Mutirangura A, Supiyaphun P, Trirekapan S, et al : Telomerase activity in oral leukoplakia and head and neck squamous cell carcinoma. Cancer Res. 1996 ; 56 : 3530-3533   PUBMED
12 Harley CB : Telomereloss : Mitotic clock or genetic time bomb? Mutation Res. 1991 ; 256 : 271-282   DOI   ScienceOn
13 Blackburn DH : Structure and function of telomeres. Nature. 1991 ; 350 : 569-573   DOI   PUBMED   ScienceOn
14 Jung KY, Choi JO : Clinical significance of the expression of oncosuppressor gene proteins and epidermal growth factor receptor in squamous cell carcinoma ofthe larynx. Korean J Otolaryngol. 1993 ; 36 : 990-1004
15 Harrington L, McPhail T, Mar V, et al : A mammalian telomerase-assocoated protein. Science. 1997 ; 275 : 973-977   DOI   PUBMED   ScienceOn
16 Tahara H, Yasui W, Thara E, et al : Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections. Oncogene. 1999 ; 18 : 1561-1567   DOI   ScienceOn
17 Toshiknni N, Nouso K, Higashi T, et al : Expression oftelomerase-associated protein 1 and telomerase reverse transcriptase in hepatocelluar carcinoma. Br J Cancer. 2000 ; 82 : 833-837   DOI   ScienceOn
18 Mao Li, EL-Naggar AK, Fan YH, et al : Telomerase activity in head and neck squamous cell carcinoma and adjacent tissue. Cancer Res. 1996 ; 56 : 5600-5604
19 Shay JW, Bacchetti S : A survey oftelomerase activity in human cancer. Eur J cancer. 1997 ; 33 : 787-791.   DOI   ScienceOn