Browse > Article
http://dx.doi.org/10.14400/JDC.2015.13.8.503

Suppressive effects of Th2 cytokines expression and the signal transduction mechanism in MC/9 mast cells by flavonol derived from Ginkgo biloba leaves  

Kwon, Hae-Young (Dept. of Cosmetology, Kookje College)
Chung, Kyu-Jin (College of Pharmacy, Chungnam National University)
Cheong, Kwang-Jo (Dept. of Beauty Health Care, Daejon University)
Publication Information
Journal of Digital Convergence / v.13, no.8, 2015 , pp. 503-514 More about this Journal
Abstract
The effects of Flavonol contents from Ginkgo biloba leaf on anti-atopy activity have not rarely been verified. This study is to investigate the effects of flavonol on Th2 cytokine production in MC/9 mast cells. For this, flavonol was analyzed by ELISA and Real-time PCR. Analysis results showed that flavonol significantly suppressed production of Th2 cytokines(IL-13, MIP-1a) in a dose dependent manner. The mRNA expression of IL-4, IL-5, IL-13, TNF-a were effectively restrained by Flavonol at the concentration 25,50,$100{\mu}g/m{\ell}$. And decrease of expression of NFAT-1, c-jun protein was confirmed by western blot analysis. These results indicate that flavonol has effects of decreasing the Th2 cytokine production in the MC/9 mast cell causing inhibition of transcription factors such as NFAT-1, c-jun. Thus, we would like to brief that flavonol may have the applicability as therapeutic agent for atopic dermatitis.
Keywords
Flavonol; Ginkgo biloba leaves; MC/9 mast cell; Th2 cytokine; atopic dermatitis;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Taketomi Y, Murakami M. Lipid networks in m-ast cell biology. Yakugaku Zasshi, Vol. 131, No. 1, pp.73-84, 2001.   DOI
2 An HS. Textbook of pediatrics. 9th ed. Seoul: Korea Textbook Publishing Co. pp. 1104-1121, 2010.
3 The Korean Academy of Asthma, Allergy and Cl-inical Immunology. Asthma and allergic disease. Seoul: Koonja Pub. pp.13-116, 2002.
4 Shin HW. The experimental study on the asthma suppressive & immune response of armeniacae am-arum semen herbal-acupuncture at ST36 in OVA-induced asthma mouse. Grad School Daejeon Univ. 2006
5 Jung-Hee Kim, Current Understanding of Atopic Dermatitis, Pediatric allergy and respiratory disease, Vol. 14, No. 1, pp. 12-23, 2004.
6 Masuda A, Yoshikai Y, Kume H, Matsuguchi T. The Interaction between GATA Proteins and Acti-vator Protein-1 Promotes the Transcription of IL-13 in Mast Cells, J Immunol, Vol. 173, No. 9, pp. 5564-5573, 2004.   DOI
7 Klein M, Klein-Hessling S, Palmetshofer A, Serfling E, Tertilt C, Bopp T, Heib V, Becker M, Taub-e C, Schild H, Schmitt E, Stassen M. Specific and redundant roles for NFAT transcription factors in the expression of mast cell-derived cytokines, J Immunol, Vol. 177, No. 10, pp. 6667-6674, 2006.   DOI
8 Lewin I, Nechushtan H, Ke Q, Razin E : Regulati-on of AP-1 expression and activity in antigen sti-mulated mast cells: the role played by protein kin-ase C and the possible involvement of Fos inter-acting protein, Blood, Vol. 82, No. 12, pp. 3745-3751, 1993.
9 Lee JS, Yun SJ, Lee JB, Kim SJ, Won YH, Lee SC. The Efficacy of Cyclosporin in Patients with Atopic Dermatitis, Korean journal of dermatology, Vol. 46, No. 2, pp. 224-230, 2008.
10 Daniels J and Harper J, The epidemiology of atopic dermatitis. Hosp Med, Vol. 63, No. 11, pp. 649-652. 2002.   DOI
11 Finlay AY, Quality of life in atopic dermatitis, J Am Acad Dermatol., Vol. 45, pp.64-66, 2001.   DOI   ScienceOn
12 Lim KK, Su WP, Schroeter AL, Sabers CJ, Abraham RT, Pittelkow MR : Cyclosporine in the treatment of dermatologic disease: an update, Mayo Clin Proc, 71(12):1182-1191, 1996.   DOI   ScienceOn
13 Jeong, Seung-Il Choi, Byung-Min Yun, Young-Gab Lee, Jang-Won Jang, Seon-Il, A Noble Therapeutic Approach of Atopic dermatitis by Development of Th2 Chemokine Inhibitors from Natural Products : Inhibitory Effect of Sophora flavescens Extract in Atopic Dermatitis Model mice, NC/Nga, Herbal formula science, Vol.17 no.1, pp.141-151, 2009.
14 Sung Ha Yun , Sang Sun Lee, Jung Eun Jang, Geon Woong Noh. Sensory Evaluation of Chungkukjangs with Herbal Extracts and Clinical Evaluation in Atopy Dermatitis Patients, The Korean journal of nutrition, Vol. 37 no.8, pp.669-674, 2004.
15 R.Rouseff, Citrus Nutrition and Quality, American chemical society New York, pp.83-107, 1980.
16 Im Seon Lee, Nguyen Tien Dat, Xing Fu Cai, Guanghai Shen, and Young ho Kim. Inhibitory Effect-s of Natural Products against NFAT(nuclear fact-or of activated T cells) Transcription Factor, Kor. J. Pharmacogn. Vol. 34, No. 2, pp. 150-155, 2003.
17 Lee M, Park J. Regulation of NFAT activation: a potential therapeutic target for immunosuppression, Mol Cells, Vol. 22, No. 1, pp. 1-7, 2006.   DOI   ScienceOn
18 Park JC. Transcription Factor NFAT: The Regulation in Immune Response. KSBMB News. Vol. 20, No. 2, pp. 1-7, 2000.
19 Rooney JW, Hoey T, Glimcher LH. Coordinate a-nd Cooperative Roles for NFAT and AP-1 in the Regulation of the Murine IL-4 Gene. Immun. Vol. 2, pp. 473-83, 1995.
20 Park, Gye YoungLee, Seung HeeHwangbo, BinYim, Jae-JoonLee, Choon-TaekKim, Young WhanHan, Sung KooShim, Young-SooYoo, Chul-Gyu. Proinflammatory Cytokine Expression Through $NF-{\kappa}B/I-{\kappa}B$ Pathway in Lung Epithelial Cells. Tuberculosis and Respiratory Diseases. Vol. 49, No. 3, pp. 332-342, 2000.   DOI
21 Jung Im Kim, Young Hee Choi, and Oh Kyung Kwon. Dyeability and Antibacterial Activity of Gin-kgo Biloba Leaf Extract Using Three Kinds of Aqueous Extraction Solvents. Journal of the Korean Society of Dyers and Finishers, Vol. 16, No. 2, pp. 8-14, 2004.
22 Sam Sik Kang, Ju Sun Kim, Wie-jong Kwak and Ki-hyup Kim, Flavonoids from the Leaves of Gin-kgo biloba, Kor. J. Pharmacogn. Vol. 21, No. 2, pp.111-120, 1990.
23 Sam Sik Kang, Ju Sun Kim, Wie-jong Kwak and Ki-hyup Kim. Identification and Quantitative An-alysis of Flavonol Glycosides from Ginkgo bilob-a Leaves by High Performance Liquid Chromato-graphy, Kor. J. Pharmacogn., Vol. 21, No. 2, pp. 148-152,1990.
24 Young-Suk Kwon, Hyun-Hok Cho, Seong-Yun Jeong, Sang-Youb Lee, Min-ju Kim, Sun-Ja Cho and Sang-Joon Lee, Antibacterial Activities of Gink-go Biloba Leaves Extracts Against Isolated Bact-eria from Museums, Journal of the Environmental Scie-nces, Vol. 15, No. 10, pp.983-388, 2006.   DOI   ScienceOn
25 In-Hwa Lee, Youn Shim, Seung-Hyun Choi, Ju-Young Park, Sung-Woo Han, Jn-Young Song, Suk-Jin Yoon. A Study on the Antimicrobial Effect of Ginkgo biloba Leaves Extracts according to Con-centrations of Ethanol for staphylococcus aureus, KSBB Journal , Vol. 21 No. 4, pp. 312-316, 2006.
26 Ishiyama M, Miyazono Y, Sasamoto K, Ohkura Y, Ueno K. A highly water-soluble disulfonated te-trazolium salt as a chromogenic indicator for NADH as well as cell viability, Talanta, Vol. 44, No. 7, pp. 1299-1305, 1997.   DOI   ScienceOn
27 Sandstrom MH, Faergemann J. Prognosis and pr-ognostic factors in adult patient with atopic derm-atitis : a long-term follow-up qeustionnaire study. Br J Dermatol. Vol. 150, pp. 103-110, 2004.   DOI   ScienceOn
28 Ahn KM. Role of mast cells in allergic inflammat-ion and innate immunity. Korean J Pediatr. Vol. 47, pp. 1137-1141, 2004.