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http://dx.doi.org/10.15268/ksim.2019.7.2.141

Antimicrobial and Cell Viability Measurement of Hypochlorous Acid against Streptococcus. mutans and Aggregatibacter. actinomycetemcomitans  

Song, Jiyeon (Dept. of Dental Health Sciences, Graduate School of Dongseo University)
Kim, Jiyoung (Dept. of Dental Hygiene, Kyungnam College of Information & Technology)
Lee, Kyunghee (Dept. of Dental Health Sciences, Graduate School of Dongseo University)
Publication Information
Journal of The Korean Society of Integrative Medicine / v.7, no.2, 2019 , pp. 141-151 More about this Journal
Abstract
Purpose : Hypochlorous acid (HOCl), a major inorganic bactericidal compound of innate immunity, is effective against a broad range of microorganisms. In particular, HOCl is well-known as a non-antibiotic antimicrobial substance. However, effects of HOCl as an antimicrobial agent are still needed to study these functions against various specific type of microorganisms. In this study, we investigated the antimicrobial effect of hypochlorous acid (HOCl) in S. mutans and A. actinomycetemcomitans to cause dental caries and periodontal disease. Experiments were conducted to observe whether HOCl become effective replacement of disinfectant. Methods : To observe antimicrobial effect of HOCl, stabilized HOCl is prepared in the form of a physiologically balanced solution in pre-conditioned and post-conditioned HOCl solution. As a control, commercially available disinfectant MAXCLEAN was used as positive control. Moreover, S. mutans and A. actinomycetemcomitans distribution in gagrin, filtered tap water, and culture media. Cell viability were measured by viable cell count methods and disk diffusion test. Results : Our results showed that treatment of HOCl have no effect against antimicrobial effect compare to control group especially gagrin in disk diffusion test. HOCl tended to reduced viability against S. mutans in group of post-conditioned than pre-conditioned of HOCl solution however, there was no significant difference as well as no effect in A. actinomycetemcomitans. Conclusion : HOCl showed tendency to reduce viability against S. mutans in group of post-conditioned of HOCl solution and no effect of antimicrobial effect. Although HOCl is well known as effective against a broad range of microorganisms, HOCl seems to have diversity following type of species to be used as antimicrobial drug following our results. Therefore, it is necessary to be rigidly controlled and regulated in using HOCl solution clinically.
Keywords
dental clinic; hypochlorous acid; HOCL; oral bacteria; oral gargling;
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Times Cited By KSCI : 13  (Citation Analysis)
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