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Changes in the Physical Properties of Irradiated Red Blood Cells  

Kim, Yu-Kyung (Department of Clinical Pathology, School of Medicine, Kyungpook National University)
Park, Jung-Ran (Department of Clinical Pathology, School of Medicine, Kyungpook National University)
Kim, Dong-Hyun (Department of Clinical Pathology, School of Medicine, Kyungpook National University)
Kwon, Eun-Hee (Department of Clinical Pathology, School of Medicine, Kyungpook National University)
Kim, Eun-Jin (Red Cross Daegu-Kyungpook Blood Center)
Won, Dong-Il (Department of Clinical Pathology, School of Medicine, Kyungpook National University)
Shin, Se-Hyun (Department of Mecahnical Engineering, School of Engineering, Korea University)
Suh, Jang-Soo (Department of Clinical Pathology, School of Medicine, Kyungpook National University)
Publication Information
The Korean Journal of Blood Transfusion / v.18, no.3, 2007 , pp. 129-137 More about this Journal
Abstract
Background: Irradiation of cellular blood products is the current method used for the prevention of transfusion-associated graft-versus-host disease. However, irradiation has been shown to cause biochemical changes in stored red blood cells (RBCs) and to generate reactive oxygen species (ROS). Irradiation-induced biochemical changes and oxidation damage of the RBCs is closely related to the deformability of these cells. Furthermore, deformation of the RBCs may lead to alterations in the post-transfusion viability of stored RBCs. In this study, we evaluated the physical properties of irradiated RBCs. Methods: Citrate phosphate dextrose adenine-1 preserved RBC concentrates were irradiated with a minimum dose of 25 Gy and stored at 4oC for 28 days. The deformation of the RBCs was measured by a microfluidic ektacytometer (RheoScan-D). To examine the effect of oxidative stress, the RBC concentrates were exposed to oxidative stress using FeSO4 and ascorbic acid.Results: The deformability threshold of the irradiated RBCs was significantly lower than that of the control RBCs on day 21 of storage and later (P＜0.05). The deformability threshold of the RBCs exposed to oxidative stress was lower than that of control RBCs, and the difference was significant on day 21 of storage and later (P＜0.05). For up to 21 days of storage, the deformability of control and irradiated RBCs were maintained; however, they were significantly decreased at 28 days of storage compared with 1 day of storage (P＜0.05).Conclusion: Irradiation reduced the threshold of RBC deformability during storage. In accordance with the effect of irradiation, oxidative stress affected the RBC deformability. Therefore, a ROS scavenger may play a protective role against deformation of irradiated RBC concentrates. Further evaluation will be required for confirmation and clarification of these findings. (Korean J Blood Transfus 2007;18:129-137)
Keywords
RBC deformability; Irradiation; Oxidative stress;
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Times Cited By KSCI : 1  (Citation Analysis)
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