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http://dx.doi.org/10.3345/kjp.2012.55.9.337

Excess of leptin inhibits hypothalamic KiSS-1 expression in pubertal mice  

Ahn, Sung-Yeon (Department of Pediatrics, Kangwon National University School of Medicine)
Yang, Sei-Won (Department of Pediatrics, Seoul National University College of Medicine)
Lee, Hee-Jae (Department of Pharmacology, Kangwon National University School of Medicine)
Byun, Jong-Seon (Department of Pharmacology, Kangwon National University School of Medicine)
Om, Ji-Yeon (Department of Pharmacology, Kangwon National University School of Medicine)
Shin, Choong-Ho (Department of Pediatrics, Seoul National University College of Medicine)
Publication Information
Clinical and Experimental Pediatrics / v.55, no.9, 2012 , pp. 337-343 More about this Journal
Abstract
Purpose: Leptin has been considered a link between metabolic state and reproductive activity. Defective reproductive function can occur in leptin-deficient and leptin-excessive conditions. The aim of this study was to examine the effects of centrally injected leptin on the hypothalamic KiSS-1 system in relation to gonadotropin-releasing hormone (GnRH) action in the initial stage of puberty. Methods: Leptin (1 ${\mu}g$) was injected directly into the ventricle of pubertal female mice. The resultant gene expressions of hypothalamic GnRH and KiSS-1 and pituitary LH, 2 and 4 hours after injection, were compared with those of saline-injected control mice. The changes in the gene expressions after blocking the GnRH action were also analyzed. Results: The basal expression levels of KiSS-1, GnRH, and LH were significantly higher in the pubertal mice than in the prepubertal mice. The 1-${\mu}g$ leptin dose significantly decreased the mRNA expression levels of KiSS-1, GnRH, and LH in the pubertal mice. A GnRH antagonist significantly increased the KiSS-1 and GnRH mRNA expression levels, and the additional leptin injection decreased the gene expression levels compared with those in the control group. Conclusion: The excess leptin might have suppressed the central reproductive axis in the pubertal mice by inhibiting the KiSS-1 expression, and this mechanism is independent of the GnRH-LH-estradiol feedback loop.
Keywords
Leptin; KiSS-1; Gonadotropin-releasing hormone; Luteinizing hormone; Puberty;
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