Browse > Article
http://dx.doi.org/10.3345/kjp.2009.52.9.1015

Toll-like receptor 9 expression and interferon-α secretion upon CpG-ODN stimulation in allergic subjects  

Han, Man Yong (Departments of Pediatrics, CHA University School of Medicine)
Jee, Hye Mi (Departments of Pediatrics, CHA University School of Medicine)
Kim, Hyeong Yoon (Departments of Pediatrics, CHA University School of Medicine)
Lee, Cho Ae (Departments of Pediatrics, CHA University School of Medicine)
Cho, Hyo-Jin (Departments of Internal Medicine, CHA University School of Medicine)
Hwang, Seong-Gyu (Departments of Internal Medicine, CHA University School of Medicine)
Kim, Kyu-Earn (Department of Pediatrics, Yonsei University College of Medicine)
Publication Information
Clinical and Experimental Pediatrics / v.52, no.9, 2009 , pp. 1015-1020 More about this Journal
Abstract
Purpose:The aim of this study is to explore the effect of the Toll-like receptor 9 (TLR9) expressed in plasmacytoid dendritic cells (pDCs) that respond to antigen to Th2 immune deviation in allergic patients. Methods:Subjects consisted of 19 allergic patients and 17 healthy volunteers. Skin prick tests and nasal provocation tests were performed for the two groups. Peripheral blood mononuclear cells (PBMCs) were collected from subjects and analyzed for the Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56) (-), HLA-DR (+), and CD123 (+) using flow cytometry. In addition, we analyzed TLR9 mRNA by reverse transcriptase-polymerase chain reaction. The level of $interferon-{\alpha}$ ($IFN-{\alpha}$) of the PBMCs following stimulation with the TLR9 ligand CpG-ODN 2216 was also evaluated. Results:Analyses of CD123 (+) revealed a nearly similar distribution for the classical pDC markers in the allergic group ($0.1%{\pm}0.04%$) and in the controls ($0.25%{\pm}0.23%$). The mRNA levels of TLR9 on PBMCs were not different between the allergic group and the controls ($1.29{\pm}0.41$ vs. $1.25{\pm}0.23$, respectively). Additionally, the level of $IFN-{\alpha}$ in PBMCs exposed to stimuli of the TLR9 ligand CpG-ODN 2216 was not significantly different between the two groups ($911{\pm}829$ vs. $1,095{\pm}888pg/mL$, respectively). Conclusion:We found no evidence that TLR9-dependent immune responses in human pDCs are associated with allergic status.
Keywords
Allergy; Toll-like receptor 9; Plasmacytoid dendritic cell; Interferon-alpha;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim HY, Han MY, Seo JY, Cho HJ, Kim NK, Oh DY, et al. IL-12 production in lipopolysaccharide- and Dermatophagoides pteronyssinus-copulsed monocyte derived-dendritic cells. J Asthma Allergy Clin Immunol 2006;26:1-6
2 Fageras Bottcher M, Hmani-Aifa M, Lindstrom A, Jenmalm MC, Mai XM, Nilsson L, et al. A TLR4 polymorphism is associated with asthma and reduced lipopolysaccharide- induced interleukin-12 (p70) responses in Swedish children. J Allergy Clin Immunol 2004;114:561-7   DOI   ScienceOn
3 Novak N, Allam JP, Hagemann T, Jenneck C, Laffer S, Valenta R, et al. Characterization of FcεRI-bearing CD123 blood dendritic cell antigen-2 plasmacytoid dendritic cells in atopic dermatitis. J Allergy Clin Immunol 2004;114:364-70   DOI   ScienceOn
4 Fitzgerald-Bocarsly P, Dai J, Singh S. Plasmacytoid dendritic cells and type I IFN: 50 years of convergent history. Cytokine Growth Factor Rev 2008;19:3-19   DOI   ScienceOn
5 Berghofer B, Frommer T, Konig IR, Ziegler A, Chakraborty T, Bein G, et al. Common human Toll-like receptor 9 polymorphisms and haplotypes: association with atopy and functional relevance. Clin Exp Allergy 2005;35:1147-54   DOI   ScienceOn
6 Yang IA, Fong KM, Holgate ST, Holloway JW. The role of Toll-like receptors and related receptors of the innate immune system in asthma. Curr Opin Allergy Clin Immunol 2006;6:23-8   DOI   ScienceOn
7 Tversky JR, Le TV, Bieneman AP, Chichester KL, Hamilton RG, Schroeder JT. Human blood dendritic cells from allergic subjects have impaired capacity to produce interferon-alpha via Toll-like receptor 9. Clin Exp Allergy 2008;38:781-8   DOI   ScienceOn
8 Umetsu DT, DeKruyff RH. TH1 and TH2 CD4+ cells in human allergic diseases. J Allergy Clin Immunol 1997;100: 1-6   DOI   PUBMED   ScienceOn
9 Kaisho T, Akira S. Toll-like receptor function and signaling. J Allergy Clin Immunol 2006;117:979-87   DOI   ScienceOn
10 Reider N, Reider D, Ebner S, Holzmann S, Herold M, Fritsch P, et al. Dendritic cells contribute to the development of atopy by an insufficiency in IL-12 production. J Allergy Clin Immunol 2002109:89-95   DOI   ScienceOn
11 Cook DN, Pisetsky DS, Schwartz DA. Toll-like receptors in the pathogenesis of human disease. Nat Immunol 2004;5: 975-9   DOI   ScienceOn
12 Rissoan MC, Soumelis V, Kadowaki N, Grouard G, Briere F, de Waal Malefyt R, et al. Reciprocal control of T helper cell and dendritic cell differentiation. Science 1999;283:1183-6   DOI   PUBMED   ScienceOn
13 Lazarus R, Klimecki WT, Raby BA, Vercelli D, Palmer LJ, Kwiatkowski DJ, et al. Single-nucleotide polymorphisms in the Toll-like receptor 9 gene (TLR9): frequencies, pairwise linkage disequilibrium, and haplotypes in three U.S. ethnic groups and exploratory case-control disease association studies. Genomics 2003;81:85-91   DOI   ScienceOn
14 Deering RP, Orange JS. Development of a clinical assay to evaluate Toll-like receptor function. Clin Vaccine Immunol 2006;13:68-76   DOI   ScienceOn
15 Triantafilou M, Triantafilou K. The dynamics of LPS recognition: complex orchestration of multiple receptors. J Endotoxin Res 2005;11:5-11   PUBMED   ScienceOn