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http://dx.doi.org/10.3345/kjp.2009.52.11.1234

Molecular identification and clinical features of enteroviral infection in children of central Korea: An overview of enteroviral epidemiology between spring 2005 and autumn 2006  

Roh, Eui Jung (Department of Pediatrics, College of Medicine, Konyang University Daejeon)
Jin, Yong Man (Department of Pediatrics, College of Medicine, Dankook University)
Chung, Eun Hee (Department of Pediatrics, College of Medicine, Dankook University)
Chang, Young Pyo (Department of Pediatrics, College of Medicine, Dankook University)
Park, Woo Sung (Department of Pediatrics, College of Medicine, Dankook University)
Park, Kwisaung (Chungcheongnam-Do Health and Environment Research Institute)
Jee, Young Mee (Division of Enteric and Hepatitis Viruses, Department of Virology, National Institute of Health, Korea Center for Disease Control and Prevention)
Publication Information
Clinical and Experimental Pediatrics / v.52, no.11, 2009 , pp. 1234-1240 More about this Journal
Abstract
Purpose:Enteroviruses (EVs) are commonly known to cause infection, especially in infants and children. This report presents an overview of enterovirus epidemiology in central Korea. Methods:From the spring of 2005 to the autumn of 2006, we collected the cerebrospinal fluid (CSF) and stool samples from the pediatric patients with a febrile illness or suspected meningitis who were admitted to hospitals in central Korea. In order to test for EVs, cell lines were derived from pretreated susceptible specimen, and the cytopathic effects were observed. Seminested real time-polymerase chain reaction (RT-PCR) and direct sequencing were performed for genotypic and phylogenetic analyses. Results:Of the 305 patients examined, 51 (16.7%) tested positive for EV. Of these 51 patients, 44 showed the following serotypes: Echovirus (ECV) 18 (18 cases, 35.2%), Coxsackievirus B (CVB) 5 (13 cases, 25.4%), ECV25 (5 cases, 9.8%), ECV9 (4 cases, 7.8%), ECV5 (3 cases, 5.8%), and EV74 (1 case, 1.9%). In 2005, between June and August, ECV18 and CVB5 were mostly responsible for the enteroviral infections among the patients in central Korea. In 2006, between July and August, ECV25 was mostly the cause of enteroviral infection. Conclusions:There is a need for continuous surveillance of enteroviral infection and its clinical manifestations, particularly for EV74, which was first identified in Korea.
Keywords
Children; Enterovirus; RT-PCR; Enterovirus 74;
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