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http://dx.doi.org/10.3345/kjp.2008.51.9.971

Comparison of a whole blood Interferon-γ assay and A tuberculin skin test for detecting latent tuberculosis infection in children  

Chun, Jin-Kyong (Department of Pediatrics, College of Medicine Yonsei University)
Kim, Chang Ki (Department of Laboratory Medicine, College of Medicine Yonsei University)
Kim, Hyun Sook (Department of Laboratory Medicine, College of Medicine Yonsei University)
Jung, Ghee Young (Sowha Childrens Hospital)
Linton, John A. (Department of Family Medicine, College of Medicine Yonsei University)
Kim, Ki Hwan (Department of Pediatrics, College of Medicine Yonsei University)
Lee, Taek Jin (Department of Pediatrics, College of Medicine Yonsei University)
Jeon, Ji Hyun (Department of Pediatrics, College of Medicine Yonsei University)
Kim, Dong Soo (Department of Pediatrics, College of Medicine Yonsei University)
Publication Information
Clinical and Experimental Pediatrics / v.51, no.9, 2008 , pp. 971-976 More about this Journal
Abstract
Purpose : Surveillance for detecting and managing latent tuberculosis infection (LTBI) is a key component of tuberculosis control. The classic surveillance tool, the tuberculin skin test (TST), may have some limitations when used in the Bacillus Calmette-$Gu{\acute{e}}rin$ (BCG)-vaccinated population. The object was to perform a blood test $QuantiFERON^{(R)}$-TB Gold In Tube (QFT-G IT) based on the detection of interferon-$\gamma$ ($IFN-{\gamma}$) released by T cells in response to Mycobacterium tuberculosis-specific antigens, and to compare the efficacy of this new diagnostic tool for LTBI with that of TST. Methods : For six months, between October 1, 2006 and April 30, 2007, data were collected from 111 patients under 15 years of age at Severance Children's Hospital. TST and QFT-G IT tests were performed with children with or without contact histories of tuberculosis. In addition to these tests, we examined comparative data from 29 adults who had tuberculosis, to detect false negative rates in the QFT-G IT method. Results : Thirty-three children had household contact histories. In this group, 15% and 42% of cases were found to be positive using the QFT-G IT assay and TST, respectively. Agreement was low between these two tests (${\kappa}=0.39$). In the adult active tuberculosis group, the QFT-G IT false negative rate defined as a positive culture and a negative QFT-G IT result was 12.5%. Conclusion : In diagnosing LTBI in children, the usefulness of a whole-blood $IFN-{\gamma}$ assay employing TB-specific antigens will be revealed only by examining additional longitudinal clinical data; this study serves as a starting point in that process.
Keywords
Children; Tuberculin skin test; Interferon-gamma release assay;
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