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http://dx.doi.org/10.3345/kjp.2008.51.8.834

Detection and clinical manifestations of twelve respiratory viruses in hospitalized children with acute lower respiratory tract infections : Focus on human metapneumovirus, human rhinovirus and human coronavirus  

Kim, Kum Hyang (Department of Pediatrics, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Lee, Jung Ho (Department of Pediatrics, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Sun, Dong Shin (Department of Pediatrics, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Kim, Yong Bae (Department of Preventive Medicine, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Choi, Young Jin (Department of Laboratory Medicine, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Park, Joon Soo (Department of Pediatrics, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Kim, Chang Jin (Department of Pathology, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Jung, Dong Jun (Department of Pathology, Soonchunhyang University Hospital, Soonchunhyang University School of Medicine)
Publication Information
Clinical and Experimental Pediatrics / v.51, no.8, 2008 , pp. 834-841 More about this Journal
Abstract
Purpose : This study was perfomed to analyze in detail the viral etiology of acute lower respiratory tract infections (ALRI) in Cheunan, Korea by multiplex RT-PCR, including human rhinovirus (hRV) and newly identified viruses such as human metapneumovirus (hMPV) and human coronavirus (HCoV-OC43, HCoV-229E/NL63). Method : Nasopharyngeal aspirates (NPA) were collected from 863 hospitalized children with ALRI on the first day of admission at Soonchunhyang University Cheonan Hospital and analyzed by multiplex RT-PCR from December 2005 to November 2006. Results : Viral agents were detected from 474 subjects (54.9%). The identified viral pathogens were hRV 9.2%, hMPV 6.8%, HCoV-229E/NL63 1.4%, and HCoV-OC43 2.1%. Coinfections with ${\geq}2$ viruses were observed in 108 patients (22.8%). The major period of viral ALRI was the first year of life. Clinical diagnoses of viral ALRI were pneumonia (59.5%), bronchiolitis (24.7%), tracheobronchitis (11.4%), and croup (4%). The most common causes of bronchiolitis was respiratory syncytial virus B (RSV B), whereas hMPV, hRV, HCoV-229E/NL63, and HCoV-OC43 were commonly found in patients with pneumonia. The number of hMPV infections peaked between March and May 2006. HCoV-OC43 was prevalent from November to February 2006, whereas HCoV-229E and hRV were detected throughout the year. Conclusion : Although the study was confined to one year, hMPV was not detected during winter and peaked between March and April, which was not consistent with previous studies'. This present study indicates that HCoV is a less common respiratory pathogen in cases of ALRI in Korean children
Keywords
Respiratory virus; Respiratory infectionsm; RT-PCR; Children;
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