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Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells  

Kwon, Byoung Chul (Department of Pediatrics, Yonsei University College of Medicine)
Kim, Eun Soo (Department of Pediatrics, Yonsei University College of Medicine)
Kim, Kyung Won (Department of Pediatrics, Yonsei University College of Medicine)
Song, Tae Won (Department of Pediatrics, Yonsei University College of Medicine)
Sohn, Myung Hyun (Department of Pediatrics, Yonsei University College of Medicine)
Shin, Myeong Heon (Department of Parasitology,Yonsei University College of Medicine)
Kim, Kyu-Eam (Department of Pediatrics, Yonsei University College of Medicine)
Publication Information
Clinical and Experimental Pediatrics / v.48, no.8, 2005 , pp. 881-885 More about this Journal
Abstract
Purpose : Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils. Methods : Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1. Results : Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg $H_2O$ resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium. Conclusion : We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.
Keywords
Exercise-induced asthma; Hyperosmolar; EoL-1 cells;
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Times Cited By KSCI : 1  (Citation Analysis)
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