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Effect of Clove Extracts on Tyrosinase Gene Expression  

Chin, Jong-Eon (Dept. of Cosmetology, Dongkang College)
Lee, Jeong-Yong (Dept. of Physical Education, Dongshin University)
Kim, Kwan-Chun (Dept. of Environmental Administration, Gwangju Health College)
Publication Information
Journal of environmental and Sanitary engineering / v.22, no.1, 2007 , pp. 75-83 More about this Journal
Abstract
Clove extract by methanol increased expression of the tyrosinase gene on B16 mouse melanoma cells containing tyrosinase promoter. $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed expression rate of the tyrosinase gene about 138% and 245%, respectively, compared with control. At $500{\mu}g/mL$, expression rate of the extract was impossible to measurement by high cytotoxicity. The solvent fraction of methylene chloride also exhibited highly expression rate as methanol extract. However, the solvent fractions of butyl alcohol and water showed repressive effect on expression of tyrosinase gene at $500{\mu}g/mL$. In MTT assay, cell survival rate of the extract exhibited similar to expression rate of tyrosinase gene. That is, $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed the cell survival rate about 128% and 187%, respectively.
Keywords
clove; b16 mouse melanoma cell; tyrosinase gene; expression;
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1 Kim, J., Kim, S. A., Yun, W. K., Kim, E. J., Woo, M. K. and Lee, M. S. Antioxiative effect of ethanol extract for 5 kinds of spice. J. Korean Soc. Food Sci. Nutr. 33(9): 1426-1431(2004)   DOI
2 Lee, J. I., Lee, H. S., Jun, W. J., Yu, K. W., Shin, D. H., Hong, B. S., Cho, H. Y. and Yang, H. C. Anticoagulation activity pattern and in vivo test of extract from Eugenia caryophyllata. J. Korean Soc. Food Sci. Nutr. 29(3): 543-548(2000)
3 Paval, S. Dynamics of melanogenesis intermediates. J. Invest. Dermatol. 100: 162S-165S(1993)   DOI
4 Choi, B. W., Lee, B. H., Kang, K. J., Lee, E. S. and Lee, N. H. Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Korean J. Pharm. 29: 237-242(1998)
5 Baurin, N., Arnoult, E., Scior, T., Do, Q. T. and Bernard, P. Preliminary screening of some tropical plants for anti-tyrosinase activity. J. Ethnopharmacol. 82: 155-158(2002)   DOI   PUBMED   ScienceOn
6 Tomohiro, Y., Hiroyuki, N., Yasuo, K and Masako, M. The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation. Pigment Cell Research 11: 355-361(1998)   DOI   ScienceOn
7 Lee, S. H., Choi, S. Y., Kim, H. C., Hwang, J. S., Lee, B. G., Gao, J. J. and Kim, S. Y. Mulberroside F isolated from the leaves of Morus alba inhibits melanin biosynthesis. BioI. Pharm. Bull. 25(8): 1045-1048(2002)   DOI   ScienceOn
8 Lee, K. T., Lee, K. S., Jeong, J. H., Jo, B. K., Heo, M. Y. and Kim, H. P. Inhibitory effects of ramulus mori extracts on melanogenesis. J. Cosmet. Sci. 54(2): 133-142(2003)   PUBMED
9 Cho, N. C., Yoon, Y. H., Lee, H. J., Shon, H. J., Kim, Y. K., Choi, K. H., Ra, M. S., Cho, Y. K., Lee, B. H. and Chin, J. E. Effect of onion(Allium cepa L.) extract on tyrosinase gene expression. Kor. J. Food & Nutr. 14(3): 228-232(2001)
10 Lee, H. B., Bai, S. and Chin, J. E. Inhibitory effect of Lithospermum erythrorhizon extracts on melanin biosynthesis. J. Korean Soc. Food Sci. Nutr. 34(9): 1325-1329(2005)   과학기술학회마을   DOI
11 Mosmann, T. J. Rapid colormetric assay for cellular growth and survival application to proiferation and cytotoxicity assay. Immunol. Methods 63: 55-63(1983)
12 Lee, J. I., Lee, H. S., Jun, W. J., Yu, K. W., Shin, D. H., Hong, B. S., Cho, H. Y. and Yang, H. C. Screening of anticoagulant activities III extracts from edible herbs. J. Korean Soc. Food Sci. Nutr. 29(2): 335-341(2000)
13 Park, J. H., Shin, Y. G., Shin, U. K., Baek, S. K., Lee, S. K., Chung, M. H. and Park, Y. I. Tyrosinase inhibition activity of some herbal drugs. Yakhak Hoeji 41(4): 518-523(1997)
14 Park, M. K., Park, J. H., Shin, Y. G., Shin, U. K. and Kim, H. K. Chemical constituents of Eugenia caryophyllata. Yakhak Hoeji 41(2): 149-152(1997)
15 Dong, S., Jung, S. H., Moon, J. S., Rhee, S. K. and Son, J. Y. Antioxidant activities of clove by extraction solvent. J. Korean Soc. Food Sci Nutr. 33(4): 609-613   DOI
16 Chin, J. E., Sun, H. S., Lee, K. J., Choi, T. J., Ko, Y. S., Sohn, H. J, Kim, J. J, Jeon, B. H. and Lee, B. H. Inhibitory effects of some medicinal plant extracts on the tyrosinase promoter activity on B16 mouse melanoma cells. International J. Oriental Medicine 1: 6-13(2000)
17 Chin, J. E., Lee, H. S. and Kim, K. C. Effect of Mushroom extracts on tyrosinase promoter. Korean J. Sanitation 21(3): 1-8(2006)   과학기술학회마을
18 Jung, S. W., Lee, N. K., Kim, S. J. and Han, D. Screening of tyrosinase inhibitor from plants. Korean J. Food Sci Technol. 27(6), 891-896 (1995)
19 Lee, I. S. and Ha, Y. D. Effect of edible and medicinal plants on the activation of Immune cells. J. Korean Soc. Food Nutr. 23(1): 150-155(1994)
20 Chin, J. E. and Cho N. C. Effects of Artemisia capil1aris herba extracts on the tyrosinase gene activity. Thesis Collection of Dongkang College 23: 293-307
21 Lee, J. I., Lee, H. S., Jun, W. J., Yu, K. W., Shin, D. H., Hong, B. S., Cho, H. Y. and Yang, H. C. Physiological characteristics of anticoagulant fractions from Eugenia caryophyllata. J. korean Soc. Food Sci. Nutr. 29(4): 712-718(2000)
22 Jung, K. H. and Lee, E. B. Studies on the effect of the extract of Eugenia Flos on gastritic lesion. Kor. J. Food Hygiene 7(2,3): 83-89(1992)
23 Chin, J. E., Cho, N. C. and Kim, K. C. Effect of Rubus coreanum extracts on tyrosinase promoter. Korean J. Sanitation 21(3): 44-51(2006)   과학기술학회마을
24 Lee, O. H., Jung, S. H. and Son, J. Y. Antimicrobial activity of clove extract by extraction solvents. J. Korean Soc. Food Sci. Nutr. 33(3): 494-499(2004)   DOI
25 Chin, J. E. and Cho, N. C. Effect of Houttuynia cordata extracts on tyrosinase gene expression. J. Korean Soc. Food Sci. Nutr. 34(8): 1284-1288(2000)   DOI
26 Kang, S. Y., Kim, T. G., Park. M. S., Han, H. M., Jung, K. K., Kang, J. H., Moon, A. and Kim, S. H. Inhibitory effects of Eugema caryophyllata, Ephedra sinica and Cinnamomum cassia on the replication of HBV in HEPG22.2.15 cells. J. Applied Pharmacol. 7: 133-137(1999)
27 Aroca, P., Urabe, K., Kobayashi, K., Taskamoto, K. and Hearing, V. J. Melanin biosynthesis patterns of following hormonal stimulation. J. Biol. Chem. 268: 25650-25655(1993)   PUBMED
28 Isao, K. and Ikuyo, K. H. 2-Hydroxy-4-methoxy-benzaldehyd e a potent tyrosinase inhibitor from African medicinal plants. Planta Medica 65: 19-22(1999)   DOI   PUBMED
29 Jimenez-Cervantes, C. Solano, F., Kobayashi, T., Urabe, K., Hearing, V. J., Lozano, J. and Garcia-Barron, C. A new enzymatic function in the melanogenic pathway. J. Biol. Chem. 269: 17993-18001(1994)   PUBMED
30 Son, D. H., Lee, S. I. and Chung, Y. G. Antioxidative of medicinal plants on pathogenic bacteria. J Korean Soc Hygienic Science 7(2): 103-108(2001)
31 Chin, J. E. and Kim, K. C. Effect of chestnut bark extracts on tyrosinase gene expression. Korean J. Sanitation 20(3): 10-16(2005)   과학기술학회마을