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http://dx.doi.org/10.4196/kjpp.2021.25.4.297

Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53  

Zhu, Ri Zhe (Department of Biochemistry, Wonkwang University School of Medicine)
Li, Bing Si (Department of Biochemistry, Wonkwang University School of Medicine)
Gao, Shang Shang (Department of Biochemistry, Wonkwang University School of Medicine)
Seo, Jae Ho (Department of Biochemistry, Wonkwang University School of Medicine)
Choi, Byung-Min (Department of Biochemistry, Wonkwang University School of Medicine)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.25, no.4, 2021 , pp. 297-305 More about this Journal
Abstract
Luteolin, a sort of flavonoid, has been reported to be involved in neuroprotective function via suppression of neuroinflammation. In this study, we investigated the protective effect of luteolin against oxidative stress-induced cellular senescence and its molecular mechanism using hydrogen peroxide (H2O2)-induced cellular senescence model in House Ear Institute-Organ of Corti 1 cells (HEI-OC1). Our results showed that luteolin attenuated senescent phenotypes including alterations of morphology, cell proliferation, senescence-associated 𝛽-galactosidase expression, DNA damage, as well as related molecules expression such as p53 and p21 in the oxidant challenged model. Interestingly, we found that luteolin induces expression of sirtuin 1 in dose- and time-dependent manners and it has protective role against H2O2-induced cellular senescence by upregulation of sirtuin 1 (SIRT1). In contrast, the inhibitory effect of luteolin on cellular senescence under oxidative stress was abolished by silencing of SIRT1. This study indicates that luteolin effectively protects against oxidative stress-induced cellular senescence through p53 and SIRT1. These results suggest that luteolin possesses therapeutic potentials against age-related hearing loss that are induced by oxidative stress.
Keywords
Cellular senescence; Hydrogen peroxide; Luteolin; Sirtuin 1; Tumor suppressor protein p53;
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