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http://dx.doi.org/10.4196/kjpp.2018.22.4.391

Anti-inflammatory and utero-relaxant effect of α-bisabolol on the pregnant human uterus  

Munoz-Perez, Victor Manuel (Area Academica de Medicina del Instituto de Ciencias de la Salud. Universidad Autonoma del Estado de Hidalgo)
Ortiz, Mario I. (Area Academica de Medicina del Instituto de Ciencias de la Salud. Universidad Autonoma del Estado de Hidalgo)
Ponce-Monter, Hector A. (Area Academica de Medicina del Instituto de Ciencias de la Salud. Universidad Autonoma del Estado de Hidalgo)
Monter-Perez, Vicente (Hospital General de los SSH)
Barragan-Ramirez, Guillermo (Hospital General de los SSH)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.22, no.4, 2018 , pp. 391-398 More about this Journal
Abstract
The aim of this study was to evaluate the in vitro anti-inflammatory and utero-relaxant effect of ${\alpha}$-bisabolol on the pregnant human myometrium. Samples from the pregnant human myometrium were used in functional tests to evaluate the inhibitory effect of ${\alpha}$-bisabolol (560, 860, 1,200 and $1,860{\mu}M$) on spontaneous myometrial contractions. The intracellular cyclic adenosine monophosphate (cAMP) levels generated in response to ${\alpha}$-bisabolol in human myometrial homogenates were measured by ELISA. The anti-inflammatory effect of ${\alpha}$-bisabolol was determined through the measurement of two pro-inflammatory cytokines, tumor necrosis factor-${\alpha}$ ($TNF{\alpha}$) and interleukin $(IL)-1{\beta}$, and the anti-inflammatory cytokine IL-10, in pregnant human myometrial explants stimulated with lipopolysaccharide (LPS). Forskolin was used as a positive control to evaluate the cAMP and cytokine levels. ${\alpha}$-Bisabolol was found to induce a significant inhibition of spontaneous myometrial contractions at the highest concentration level (p<0.05). ${\alpha}$-Bisabolol caused a concentration-dependent decrease in myometrial cAMP levels (p<0.05) and a concentration-dependent decrease in LPS-induced $TNF{\alpha}$ and $IL-1{\beta}$ production, while IL-10 production did not increase significantly (p>0.05). The anti-inflammatory and utero-relaxant effects induced by ${\alpha}$-bisabolol were not associated with an increase in cAMP levels in pregnant human myometrial samples. These properties place ${\alpha}$-bisabolol as a potentially safe and effective adjuvant agent in cases of preterm birth, an area of pharmacological treatment that requires urgent improvement.
Keywords
${\alpha}$-bisabolol; cAMP; Immunomodulation; Inflammation; Preterm labor;
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