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http://dx.doi.org/10.4196/kjpp.2016.20.3.269

Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts  

Baek, Beomyeol (Department of Biochemistry, College of Natural Sciences, Kangwon National University)
Lee, Su Hee (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
Kim, Kyunghoon (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
Lim, Hye-Won (Shebah Biotech Inc.)
Lim, Chang-Jin (Department of Biochemistry, College of Natural Sciences, Kangwon National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.20, no.3, 2016 , pp. 269-277 More about this Journal
Abstract
Ellagic acid (EA), an antioxidant polyphenolic constituent of plant origin, has been reported to possess diverse pharmacological properties, including anti-inflammatory, anti-tumor and immunomodulatory activities. This work aimed to clarify the skin anti-photoaging properties of EA in human dermal fibroblasts. The skin anti-photoaging activity was evaluated by analyzing the reactive oxygen species (ROS), matrix metalloproteinase-2 (MMP-2), total glutathione (GSH) and superoxide dismutase (SOD) activity levels as well as cell viability in dermal fibroblasts under UV-B irradiation. When fibroblasts were exposed to EA prior to UV-B irradiation, EA suppressed UV-B-induced ROS and proMMP-2 elevation. However, EA restored total GSH and SOD activity levels diminished in fibroblasts under UV-B irradiation. EA had an up-regulating activity on the UV-B-reduced Nrf2 levels in fibroblasts. EA, at the concentrations used, was unable to interfere with cell viabilities in both non-irradiated and irradiated fibroblasts. In human dermal fibroblasts, EA plays a defensive role against UV-B-induced oxidative stress possibly through an Nrf2-dependent pathway, indicating that this compound has potential skin antiphotoaging properties.
Keywords
Ellagic acid; Glutathione; Superoxide dismutase; UV-B radiation;
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