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http://dx.doi.org/10.4196/kjpp.2016.20.2.169

High glucose and palmitate increases bone morphogenic protein 4 expression in human endothelial cells  

Hong, Oak-Kee (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Yoo, Soon-Jib (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Son, Jang-Won (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Kim, Mee-Kyoung (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Baek, Ki-Hyun (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Song, Ki-Ho (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Cha, Bong-Yun (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Jo, Hanjoong (Coulter Department of Biomedical Engineering at Georgia Tech and Emory University)
Kwon, Hyuk-Sang (Department of Internal Medicine, College of Medicine, The Catholic University of Korea)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.20, no.2, 2016 , pp. 169-175 More about this Journal
Abstract
Here, we investigated whether hyperglycemia and/or free fatty acids (palmitate, PAL) affect the expression level of bone morphogenic protein 4 (BMP4), a proatherogenic marker, in endothelial cells and the potential role of BMP4 in diabetic vascular complications. To measure BMP4 expression, human umbilical vein endothelial cells (HUVECs) were exposed to high glucose concentrations and/or PAL for 24 or 72 h, and the effects of these treatments on the expression levels of adhesion molecules and reactive oxygen species (ROS) were examined. BMP4 loss-of-function status was achieved via transfection of a BMP4-specific siRNA. High glucose levels increased BMP4 expression in HUVECs in a dose-dependent manner. PAL potentiated such expression. The levels of adhesion molecules and ROS production increased upon treatment with high glucose and/or PAL, but this eff ect was negated when BMP4 was knocked down via siRNA. Signaling of BMP4, a pro-inflammatory and pro-atherogenic cytokine marker, was increased by hyperglycemia and PAL. BMP4 induced the expression of inflammatory adhesion molecules and ROS production. Our work suggests that BMP4 plays a role in atherogenesis induced by high glucose levels and/or PAL.
Keywords
Bone morphogenic protein 4; Diabetes mellitus; Free fatty acid; High glucose;
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