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http://dx.doi.org/10.4196/kjpp.2015.19.1.21

Anti-inflammatory, Antioxidant and Antimicrobial Effects of Artemisinin Extracts from Artemisia annua L.  

Kim, Wan-Su (Department of Periodontology, College of Dentistry, Wonkwang University)
Choi, Woo Jin (Department of Microbiology, College of Medicine, Chung-Ang University)
Lee, Sunwoo (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Kim, Woo Joong (Department of Microbiology, College of Medicine, Chung-Ang University)
Lee, Dong Chae (Department of Microbiology, College of Medicine, Chung-Ang University)
Sohn, Uy Dong (Asia Pacific International School)
Shin, Hyoung-Shik (Department of Periodontology, College of Dentistry, Wonkwang University)
Kim, Wonyong (Department of Microbiology, College of Medicine, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.19, no.1, 2015 , pp. 21-27 More about this Journal
Abstract
The anti-inflammatory, antioxidant, and antimicrobial properties of artemisinin derived from water, methanol, ethanol, or acetone extracts of Artemisia annua L. were evaluated. All 4 artemisinin-containing extracts had anti-inflammatory effects. Of these, the acetone extract had the greatest inhibitory effect on lipopolysaccharide-induced nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), and proinflammatory cytokine ($IL-1{\beta}$, IL-6, and IL-10) production. Antioxidant activity evaluations revealed that the ethanol extract had the highest free radical scavenging activity, ($91.0{\pm}3.2%$), similar to ${\alpha}$-tocopherol (99.9%). The extracts had antimicrobial activity against the periodontopathic microorganisms Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum subsp. animalis, Fusobacterium nucleatum subsp. polymorphum, and Prevotella intermedia. This study shows that Artemisia annua L. extracts contain anti-inflammatory, antioxidant, and antimicrobial substances and should be considered for use in pharmaceutical products for the treatment of dental diseases.
Keywords
Anti-inflammatory effect; Anti-microbial activity; Antioxidant activity; Artemisinin;
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1 Taixiang W, Munro AJ, Guanjian L. Chinese medical herbs for chemotherapy side effects in colorectal cancer patients. Cochrane Database Syst Rev. 2005;(1):CD004540.
2 Hien TT, White NJ. Qinghaosu. Lancet. 1993;341:603-608.   DOI
3 Bhakuni RS, Jain DC, Sharma RP, Kumar S. Secondary metabolites of Artemisia annua and their biological activity. Curr Sci India. 2001;80:35-48.
4 de Vries PJ, Dien TK. Clinical pharmacology and therapeutic potential of artemisinin and its derivatives in the treatment of malaria. Drugs. 1996;52:818-836.   DOI
5 Efferth T. Willmar Schwabe Award 2006: antiplasmodial and antitumor activity of artemisinin--from bench to bedside. Planta Med. 2007;73:299-309.   DOI
6 Konkimalla VB, Blunder M, Korn B, Soomro SA, Jansen H, Chang W, Posner GH, Bauer R, Efferth T. Effect of artemisinins and other endoperoxides on nitric oxide-related signaling pathway in RAW 264.7 mouse macrophage cells. Nitric Oxide. 2008;19:184-191.   DOI
7 Ferreira JF, Luthria DL, Sasaki T, Heyerick A. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules. 2010;15:3135-3170.   DOI
8 Lawrence T, Willoughby DA, Gilroy DW. Anti-inflammatory lipid mediators and insights into the resolution of inflammation. Nat Rev Immunol. 2002;2:787-795.   DOI
9 Kaplanski G, Marin V, Montero-Julian F, Mantovani A, Farnarier C. IL-6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol. 2003;24:25-29.   DOI
10 Bosca L, Zeini M, Traves PG, Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005;208:249-258.   DOI
11 Ware CF. Network communications: lymphotoxins, LIGHT, and TNF. Annu Rev Immunol. 2005;23:787-819.   DOI
12 Halliwell B. Protection against tissue damage in vivo by desferrioxamine: what is its mechanism of action? Free Radic Biol Med. 1989;7:645-651.   DOI
13 Morrissey PA, O'Brien NM. Dietary antioxidants in health and disease. Int Dairy J. 1998;8:463-472.   DOI
14 Alma MH, Mavi A, Yildirim A, Digrak M, Hirata T. Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biol Pharm Bull. 2003;26:1725-1729.   DOI
15 Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. Lancet. 2005;366:1809-1820.   DOI
16 Slots J, Reynolds HS, Genco RJ. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980;29:1013-1020.
17 Bolstad AI, Jensen HB, Bakken V. Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microbiol Rev. 1996;9:55-71.
18 Slots J, Ting M. Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease: occurrence and treatment. Periodontol 2000. 1999;20:82-121.   DOI
19 Folin O, Denis W. On phosphotungastic-phosphomo-lybdic compounds as color ragents. J Biol Chem. 1912;12:239-243.
20 van Steenbergen TJ, Bosch-Tijhof CJ, Petit MD, Van der Velden U. Intra-familial transmission and distribution of Prevotella intermedia and Prevotella nigrescens. J Periodontal Res. 1997;32:345-350.   DOI
21 Dhingra V, Rajoli C, Narasu ML. Partial purification of proteins involved in the bioconversion of arteannuin B to artemisinin. Bioresource Technol. 2000;73:279-282.   DOI
22 Ferrari M, Fornasiero MC, Isetta AM. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods. 1990;131:165-172.   DOI
23 Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem Pharm Bull (Tokyo). 1988;36:2090-2097.   DOI
24 Wikler MA. Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. 8th ed. Wayne, Pa: Clinical and Laboratory Standards Institute; 2009.
25 Scannapieco FA. Periodontal inflammation: from gingivitis to systemic disease? Compend Contin Educ Dent. 2004;25(7 Suppl 1):16-25.
26 Tredwin CJ, Scully C, Bagan-Sebastian JV. Drug-induced disorders of teeth. J Dent Res. 2005;84:596-602.   DOI
27 Spencer AJ, Do LG. Changing risk factors for fluorosis among South Australian children. Community Dent Oral Epidemiol. 2008;36:210-218.   DOI
28 Ishiguro Y. Mucosal proinflammatory cytokine production correlates with endoscopic activity of ulcerative colitis. J Gastroenterol. 1999;34:66-74.   DOI
29 Ponchel F, Morgan AW, Bingham SJ, Quinn M, Buch M, Verburg RJ, Henwood J, Douglas SH, Masurel A, Conaghan P, Gesinde M, Taylor J, Markham AF, Emery P, van Laar JM, Isaacs JD. Dysregulated lymphocyte proliferation and differentiation in patients with rheumatoid arthritis. Blood. 2002; 100:4550-4556.   DOI
30 Bouma G, Strober W. The immunological and genetic basis of inflammatory bowel disease. Nat Rev Immunol. 2003;3:521-533.   DOI
31 Kovatcheva EG, Koleva II, Ilieva M, Pavlov A, Mincheva M, Konushlieva M. Antioxidant activity of extracts from Lavandula vera MM cell cultures. Food Chem. 2001;72:295-300.   DOI
32 Klotz L, Schmidt M, Giese T, Sastre M, Knolle P, Klockgether T, Heneka MT. Proinflammatory stimulation and pioglitazone treatment regulate peroxisome proliferator-activated receptor gamma levels in peripheral blood mononuclear cells from healthy controls and multiple sclerosis patients. J Immunol. 2005;175:4948-4955.   DOI
33 Calder PC. n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am J Clin Nutr. 2006;83(6 Suppl): 1505S-1519.   DOI
34 Kawasaki T, Fujimi S, Lederer JA, Hubbard WJ, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH. Trauma-hemorrhage induces depressed splenic dendritic cell functions in mice. J Immunol. 2006;177:4514-4520.   DOI
35 Ruberto G, Baratta MT, Biondi DM, Amico V. Antioxidant activity of extracts of the marine algal genus Cystoseira in a micellar model system. J Appl Phycol. 2001;13:403-407.   DOI
36 Juteau F, Masotti V, Bessiere JM, Dherbomez M, Viano J. Antibacterial and antioxidant activities of Artemisia annua essential oil. Fitoterapia. 2002;73:532-535.   DOI
37 Cavar S, Maksimovic M, Vidic D, Paric A. Chemical composition and antioxidant and antimicrobial activity of essential oil of Artemisia annua L. from Bosnia. Ind Crop Prod. 2012; 37:479-485.   DOI
38 Kwamin F, Gref R, Haubek D, Johansson A. Interactions of extracts from selected chewing stick sources with Aggregatibacter actinomycetemcomitans. BMC Res Notes. 2012;5:203.   DOI
39 Kanokwiroon K, Teanpaisan R, Wititsuwannakul D, Hooper AB, Wititsuwannakul R. Antimicrobial activity of a protein purified from the latex of Hevea brasiliensis on oral microorganisms. Mycoses. 2008;51:301-307.   DOI