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http://dx.doi.org/10.4196/kjpp.2014.18.4.327

Fucoidan Promotes the Reconstruction of Skin Equivalents  

Song, Yu Seok (Department of Biochemistry, Chung-Ang University College of Medicine)
Li, Hailan (Department of Biochemistry, Chung-Ang University College of Medicine)
Balcos, Marie Carmel (Department of Biochemistry, Chung-Ang University College of Medicine)
Yun, Hye-Young (Department of Biochemistry, Chung-Ang University College of Medicine)
Baek, Kwang Jin (Department of Biochemistry, Chung-Ang University College of Medicine)
Kwon, Nyoun Soo (Department of Biochemistry, Chung-Ang University College of Medicine)
Choi, Hye-Ryung (Department of Dermatology, Seoul National University Bundang Hospital)
Park, Kyoung-Chan (Department of Dermatology, Seoul National University Bundang Hospital)
Kim, Dong-Seok (Department of Biochemistry, Chung-Ang University College of Medicine)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.18, no.4, 2014 , pp. 327-331 More about this Journal
Abstract
In this study we investigated the effects of fucoidan on the proliferation of fibroblasts and the reconstruction of a skin equivalent (SE). Fucoidan significantly stimulated the proliferation of CCD-25Sk human fibroblasts and Western blot analysis demonstrated that fucoidan markedly increased the expression of cyclin D1 and decreased the expression of p27. Fucoidan was used to reconstruct SE. Immunohistochemical staining showed that the addition of fucoidan to dermal equivalents increased expression of proliferating cell nuclear antigen (PCNA) and p63. In addition, expression of ${\alpha}6$-integrin was significantly increased by fucoidan, whereas expression of ${\beta}1$-integrin, type 1 collagen, elastin, fibronectin did not markedly change. These results suggest that fucoidan has positive effects on epidermal reconstruction and will therefore be beneficial in the reconstruction of SE.
Keywords
Fibroblasts; Fucoidan; Proliferation; Skin equivalent;
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