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http://dx.doi.org/10.4196/kjpp.2014.18.1.33

Anti-Inflammatory and Anti-Superbacterial Properties of Sulforaphane from Shepherd's Purse  

Choi, Woo Jin (Department of Microbiology, College of Medicine, Chung-Ang University)
Kim, Seong Keun (Department of Microbiology, College of Medicine, Chung-Ang University)
Park, Hee Kuk (Department of Microbiology, College of Medicine, Chung-Ang University)
Sohn, Uy-Dong (College of Pharmacy, Chung-Ang University)
Kim, Wonyong (Department of Microbiology, College of Medicine, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.18, no.1, 2014 , pp. 33-39 More about this Journal
Abstract
Shepherd's purse, Capsella bursa-pastoris (L.) Medik., has been considered a health food for centuries in Asia and is known to contain the isothiocyanate compound sulforaphane. In this study, we evaluated the anti-inflammatory and antibacterial properties of a sulforaphane-containing solution (SCS) isolated from shepherd's purse. SCS had significant anti-inflammatory activity indicated by the decreased levels of nitric oxide (NO), cytokines (interleukin $1{\beta}$ [IL-$1{\beta}$], IL-6, and IL-10), and prostaglandin $E_2$ ($PGE_2$) in lipopolysaccharide-stimulated RAW 264.7 murine macrophages. In addition, SCS decreased the inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2) levels, which confirmed the anti- inflammatory activity of SCS. Further, SCS inhibited vancomycin-resistant enterococci (VRE) and Bacillus anthracis. The minimal inhibitory concentration was $250{\mu}g/ml$ for VRE and $1,000{\mu}g/ml$ for B. anthracis. Taken together, these data indicate that SCS has potential anti-inflammatory and anti-superbacterial properties, and thus it can be used as a functional food or pharmaceutical.
Keywords
Anti-inflammatory effect; Antimicrobial activity; Cytokine; RAW 264.7 murine macrophages; Shepherd's purse; Sulforaphane;
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