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http://dx.doi.org/10.4196/kjpp.2013.17.6.485

Effect of Neurosteroid Modulation on Global Ischaemia-Reperfusion-Induced Cerebral Injury in Mice  

Grewal, Amarjot Kaur (Rayat and Bahra Institute of Pharmacy)
Jaggi, Amteshwar Singh (Department of Pharmaceutical Sciences and Drug Research, Punjabi University)
Rana, Avtar Chand (Rayat and Bahra Institute of Pharmacy)
Singh, Nirmal (Department of Pharmaceutical Sciences and Drug Research, Punjabi University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.17, no.6, 2013 , pp. 485-491 More about this Journal
Abstract
The present study was designed to investigate the putative effect of neurosteroid modulation on global ischaemia-reperfusion-induced cerebral injury in mice. Bilateral carotid artery occlusion followed by reperfusion, produced a significant rise in cerebral infarct size along with impairment of grip strength and motor coordination in Swiss albino mice. Administration of carbamazepine (16 mg/kg, i.p.) before global cerebral ischaemia significantly attenuated cerebral infarct size and improved the motor performance. However, administration of indomethacin (100 mg/kg, i.p.) attenuated the neuroprotective effect of carbamazepine. Mexiletine (50 mg/kg, i.p.) did not produce significant neuroprotective effect. It may be concluded that the neuroprotective effect of carbamazepine may be due to increase in synthesis of neurosteroids perhaps by activating enzyme ($3{\alpha}$ HSD) as indomethacin attenuated the neuroprotective effect of carbamazepine. The sodium channel blocking effect of carbamazepine may not be involved in neuroprotection as mexiletine, a sodium channel blocker, did not produce significant neuroprotective effect.
Keywords
Carbamazepine; Global-ischaemia-reperfusion injury; Indomethacin; Mexiletine; Neurosteroids;
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