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http://dx.doi.org/10.4196/kjpp.2013.17.5.447

Nectandrin A Enhances the BMP-Induced Osteoblastic Differentiation and Mineralization by Activation of p38 MAPK-Smad Signaling Pathway  

Kim, Do Yeon (Department of Pharmacology and Clinical Pharmacy, College of Pharmacy, Kyung Hee University)
Kim, Go Woon (Department of Pharmacology and Clinical Pharmacy, College of Pharmacy, Kyung Hee University)
Chung, Sung Hyun (Department of Pharmacology and Clinical Pharmacy, College of Pharmacy, Kyung Hee University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.17, no.5, 2013 , pp. 447-453 More about this Journal
Abstract
Osteoblastic activity of nectandrin A was examined in C2C12 cells. Nectandrin A enhances the BMP-induced osteoblastic differentiation and mineralization, manifested by the up-regulation of differentiation markers (alkaline phosphatase and osteogenic genes) and increased calcium contents. In C2C12 cells co-transfected with expression vector encoding Smad4 and Id1-Luc reporter, nectandrin A increased Id1 luciferase activity in a concentration-dependent manner, when compared to that in BMP-2 treated cells, indicating that Smad signaling pathway is associated with nectandrin A-enhanced osteoblastic differentiation in C2C12 cells. In addition, nectandrin A activated p38 mitogen-activated protein kinase (MAPK) in time- and concentration-dependent manners, and phosphorylated form of pSmad1/5/8 and alkaline phosphatase activity were both decreased when the cells were pretreated with SB203580, a p38 MAPK inhibitor, suggesting that p38 MAPK might be an upstream kinase for Smad signaling pathway. Taken together, nectandrin A enhances the BMP-induced osteoblastic differentiation and mineralization of C2C12 cells via activation of p38 MAPK-Smad signaling pathway, and it has a therapeutic potential for osteoporosis by promoting bone formation.
Keywords
C2C12; Nectandrin A; p38 MAPK; Smad;
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