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http://dx.doi.org/10.4196/kjpp.2013.17.3.197

Anti-Oxidative Activity of Pectin and Its Stabilizing Effect on Retinyl Palmitate  

Ro, Jieun (College of Pharmacy, Chung-Ang University)
Kim, Yeongseok (College of Pharmacy, Chung-Ang University)
Kim, Hyeongmin (College of Pharmacy, Chung-Ang University)
Jang, Soung Baek (College of Pharmacy, Chung-Ang University)
Lee, Hyun Joo (College of Pharmacy, Chung-Ang University)
Chakma, Suharto (College of Pharmacy, Chung-Ang University)
Jeong, Ji Hoon (Department of Pharmacology, College of Medicine, Chung-Ang University)
Lee, Jaehwi (College of Pharmacy, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.17, no.3, 2013 , pp. 197-201 More about this Journal
Abstract
The purpose of this study was to examine the anti-oxidative activity of pectin and other polysaccharides in order to develop a cosmeceutical base having anti-oxidative effects towards retinyl palmitate (RP). The anti-oxidative stabilizing effects of pectin and other polysaccharides on RP were evaluated by DPPH assay and then the stabilizing effect of pectin on RP was examined as a function of time. Among the polysaccharides we examined, pectin exhibited a considerably higher anti-oxidative activity, with an approximately 5-fold greater DPPH radical scavenging effect compared to other polysaccharides. The DPPH radical scavenging effect of pectin increased gradually with increasing concentrations of pectin. At two different RP concentrations, 0.01 and 0.1% in ethanol, addition of pectin improved the stability of RP in a concentration dependent manner. The stabilizing effect of pectin on RP was more effective for the lower concentration of RP (0.01%, v/v). Further, degradation of RP was reduced following the addition of pectin as measured over 8 hours. From the results obtained, it can be suggested that pectin may be a promising ingredient for cosmeceutical bases designed to stabilize RP or other pharmacological agents subject to degradation by oxidation.
Keywords
Anti-oxidative effect; Cosmeceuticals; Pectin; Polysaccharides; Retinyl palmitate;
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1 Maxwell EG, Belshaw NJ, Waldron KW, Morris VJ. Pectin - An emerging new bioactive food polysaccharide. Trends Food Sci Tech. 2011;24:64-73.
2 Wong TW, Colombo G, Sonvico F. Pectin matrix as oral drug delivery vehicle for colon cancer treatment. AAPS Pharm Sci Tech. 2011;12:201-214.   DOI   ScienceOn
3 Yamaguchi T, Takamura H, Matoba T, Terao J. HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Biosci Biotechnol Biochem. 1998;62:1201-1204.   DOI   ScienceOn
4 Rice Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997;2:152-159.   DOI   ScienceOn
5 Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20:933-956.   DOI   ScienceOn
6 Denes JM, Baron A, Renard CM, Pean C, Drilleau JF. Different action patterns for apple pectin methylesterase at pH 7.0 and 4.5. Carbohydr Res. 2000;327:385-393.   DOI   ScienceOn
7 García-Ochoa F, Santos VE, Casas JA, Gomez E. Xanthangum: production, recovery, and properties. Biotechnol Adv. 2000; 18:549-579.   DOI   ScienceOn
8 Fatimi A, Tassin JF, Quillard S, Axelos MA, Weiss P. The rheological properties of silated hydroxypropylmethylcellulose tissue engineering matrices. Biomaterials. 2008;29:533-543.   DOI   ScienceOn
9 Shin-ya Y, Lee M, Hinode H, Kajiuchi T. Effects of Nacetylation degree on N-acetylated chitosan hydrolysis with commercially available and modified pectinases. Biochem Eng J. 2001;7:85-88.   DOI   ScienceOn
10 Becker TA, Kipke DR, Brandon T. Calcium alginate gel: a biocompatible and mechanically stable polymer for endovascular embolization. J Biomed Mater Res. 2001;54:76-86.   DOI   ScienceOn
11 Boehnlein J, Sakr A, Lichtin JL, Bronaugh RL. Characterization of esterase and alcohol dehydrogenase activity in skin. Metabolism of retinyl palmitate to retinol (vitamin A) during percutaneous absorption. Pharm Res. 1994;11:1155- 1159.   DOI
12 Draelos ZD. The cosmeceutical realm. Clin Dermatol. 2008; 26:627-632.   DOI   ScienceOn
13 Serri R, Iorizzo M. Cosmeceuticals: focus on topical retinoids in photoaging. Clin Dermatol. 2008;26:633-635.   DOI   ScienceOn
14 Guenin EP, Zatz JL. Skin permeation of retinyl palmitate from vescicles. J Soc Cosmet Chem. 1995;46:261-270.
15 Sorg O, Tran C, Carraux P, Grand D, Hügin A, Didierjean L, Saurat JH. Spectral properties of topical retinoids prevent DNA damage and apoptosis after acute UV-B exposure in hairless mice. Photochem Photobiol. 2005;81:830-836.   DOI   ScienceOn
16 Antille C, Tran C, Sorg O, Carraux P, Didierjean L, Saurat JH. Vitamin A exerts a photoprotective action in skin by absorbing ultraviolet B radiation. J Invest Dermatol. 2003; 121:1163-1167.   DOI   ScienceOn
17 Idson B. Vitamins in cosmetics, an update I. overview and vitamin A. Drug Cosmet Ind. 1990;146:26-91.
18 Carlotti ME, Rossatto V, Gallarate M. Vitamin A and vitamin A palmitate stability over time and under UVA and UVB radiation. Int J Pharm. 2002;240:85-94.   DOI   ScienceOn
19 Ihara H, Hashizume N, Hirase N, Suzue R. Esterification makes retinol more labile to photolysis. J Nutr Sci Vitaminol (Tokyo). 1999;45:353-358.   DOI
20 Carlotti ME, Rossatto V, Gallarate M, Trotta M, Debernardi F. Vitamin A palmitate photostability and stability over time. J Cosmet Sci. 2004;55:233-252.
21 Ihara H, Hashizume N, Hirase N, Suzue R. Esterification makes retinol more labile to photolysis. J Nutr Sci Vitaminol (Tokyo). 1999;45:353-358.   DOI
22 Sane A, Limtrakul J. Formation of retinyl palmitate-loaded poly(l-lactide) nanoparticles using rapid expansion of supercritical solutions into liquid solvents (RESOLV). J Supercrit Fluid. 2009;51:230-237.   DOI   ScienceOn
23 Carlotti ME, Rossatto V, Gallarate M, Trotta M, Debernardi F. Vitamin A palmitate photostability and stability over time. J Cosmet Sci. 2004;55:233-252.