Browse > Article
http://dx.doi.org/10.4196/kjpp.2012.16.6.399

Inhibitory Effects of ECQ on Indomethacin-Induced Gastric Damage in Rats  

Jung, Juho (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Nam, Yoonjin (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Sohn, Uy Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.16, no.6, 2012 , pp. 399-404 More about this Journal
Abstract
We investigated inhibitory effects of extract containing quercetin-3-O-${\beta}$-D-glucuronopyranoside (ECQ) extracted from Rumex Aquaticus Herba on indomethacin-induced gastric damage in Rats. Gastritis was induced in male Sprague-Dawley rats (200~220 g) by oral administration of indomethacin at a dose of 40 mg/kg. One hour before administration of indomethacin, animals were orally pretreated with ECQ at doses of 0.3, 1, 3 or 10 mg/kg. Six hours after indomethacin administration, the rats were sacrificed and the stomach was excised and opened along the greater curvature, and the surface area of gastric lesion was measured using optical microscope. Superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO) activities and malondialdehyde (MDA) levels were measured by ELISA. Western blot analysis was performed to detect protein expression of SOD-2. Linear hemorrhagic mucosal lesions were observed in the stomach 6 hours after oral administration of indomethacin. Pretreatment with ECQ significantly reduced the severity of the lesions in a dose-dependent manner. It also inhibited the reductions in SOD and CAT activities and SOD expression by the indomethacin-induced gastric damage. In addition, the pretreatment with ECQ significantly suppressed the elevation of the MPO activity and the MDA levels induced by indomethacin. These results suggest that ECQ has the inhibitory effects via antioxidative action against indomethacin-induced gastritis in rats.
Keywords
Extract; Flavonoid; Indomethacin-induced gastritis; QGC; Rats;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Wallace JL, Keenan CM, Granger DN. Gastric ulceration induced by nonsteroidal anti-inflammatory drugs is a neutrophildependent process. Am J Physiol. 1990;259:G462-G467.
2 Zhang X, Tajima K, Kageyama K, Kyoi T. Irsogladine maleate suppresses indomethacin-induced elevation of proinflammatory cytokines and gastric injury in rats. World J Gastroenterol. 2008;14:4784-4790.   DOI   ScienceOn
3 Goenka MK, Majumder S, Sethy PK, Chakraborty M. Helicobacter pylori negative, non-steroidal anti-inflammatory drugnegative peptic ulcers in India. Indian J Gastroenterol. 2011; 30:33-37.   DOI   ScienceOn
4 Banerjee RK. Nonsteroidal anti-inflammatory drugs inhibit gastric peroxidase activity. Biochim Biophys Acta. 1990;1034: 275-280.   DOI   ScienceOn
5 Mascolo N, Pinto A, Capasso F. Flavonoids, leucocyte migration and eicosanoids. J Pharm Pharmacol. 1988;40:293-295.   DOI
6 Landolfi R, Mower RL, Steiner M. Modification of platelet function and arachidonic acid metabolism by bioflavonoids. Structureactivity relations. Biochem Pharmacol. 1984;33:1525-1530.   DOI   ScienceOn
7 Scambia G, Ranelletti FO, Benedetti Panici P, Piantelli M, Bonanno G, De Vincenzo R, Ferrandina G, Pierelli L, Capelli A, Mancuso S. Quercetin inhibits the growth of a multidrugresistant estrogen-receptor-negative MCF-7 human breast-cancer cell line expressing type II estrogen-binding sites. Cancer Chemother Pharmacol. 1991;28:255-258.
8 Alarcon de la Lastra C, Martin MJ, Motilva V. Antiulcer and gastroprotective effects of quercetin: a gross and histologic study. Pharmacology. 1994;48:56-62.   DOI   ScienceOn
9 Suzuki Y, Ishihara M, Segami T, Ito M. Anti-ulcer effects of antioxidants, quercetin, alpha-tocopherol, nifedipine and tetracycline in rats. Jpn J Pharmacol. 1998;78:435-441.   DOI   ScienceOn
10 Yan XM, Joo MJ, Lim JC, Whang WK, Sim SS, Im C, Kim HR, Lee SY, Kim IK, Sohn UD. The effect of quercetin-3-O-${\beta}$-Dglucuronopyranoside on indomethacin-induced gastric damage in rats via induction of mucus secretion and down-regulation of ICAM-1 expression. Arch Pharm Res. 2011;34:1527-1534.   DOI   ScienceOn
11 Cho JH, Park SY, Lee HS, Whang WK, Sohn UD. The protective effect of quercetin-3-O-${\beta}$-D-glucuronopyranoside on ethanol-induced damage in cultured feline esophageal epithelial cells. Korean J Physiol Pharmacol. 2011;15:319-326.   DOI   ScienceOn
12 Kvietys PR, Twohig B, Danzell J, Specian RD. Ethanol-induced injury to the rat gastric mucosa. Role of neutrophils and xanthine oxidase-derived radicals. Gastroenterology. 1990;98: 909-920.   DOI   ScienceOn
13 Bandyopadhyay SK, Pakrashi SC, Pakrashi A. The role of antioxidant activity of Phyllanthus emblica fruits on prevention from indomethacin induced gastric ulcer. J Ethnopharmacol. 2000;70:171-176.   DOI   ScienceOn
14 Das D, Bandyopadhyay D, Bhattacharjee M, Banerjee RK. Hydroxyl radical is the major causative factor in stress-induced gastric ulceration. Free Radic Biol Med. 1997;23:8-18.   DOI   ScienceOn
15 Abdallah DM, El-Abhar HS, Abdel-Aziz DH. TEMPOL, a membrane- permeable radical scavenger, attenuates gastric mucosal damage induced by ischemia/reperfusion: a key role for superoxide anion. Eur J Pharmacol. 2009;603:93-97.   DOI   ScienceOn
16 Sugino N. The role of oxygen radical-mediated signaling pathways in endometrial function. Placenta. 2007;28 Suppl A:S133-S136.
17 Gaetani GF, Ferraris AM, Rolfo M, Mangerini R, Arena S, Kirkman HN. Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocytes. Blood. 1996;87: 1595-1599.
18 Jainu M, Devi CS. Gastroprotective action of Cissus quadrangularis extract against NSAID induced gastric ulcer: role of proinflammatory cytokines and oxidative damage. Chem Biol Interact. 2006;161:262-270.   DOI   ScienceOn
19 Kim Y, Kim WJ, Cha EJ. Quercetin-induced growth inhibition in human bladder cancer cells is associated with an increase in Ca-activated K Channels. Korean J Physiol Pharmacol. 2011;15:279-283.   DOI   ScienceOn
20 Lee BH, Shin TJ, Hwang SH, Choi SH, Kang J, Kim HJ, Park CW, Lee SH, Nah SY. Inhibitory effects of quercetin on muscle-type of nicotinic acetylcholine receptor-mediated ion currents expressed in xenopus oocytes. Korean J Physiol Pharmacol. 2011;15:195-201.   DOI   ScienceOn
21 Mart'ianov AA, Emel'ianova TG, Obukhova MF, Volkova NV, Riabinina MA, Vakulina OP, Sakharov IIu, Ashmarin IP. Physiologic effects of active immunization with triiodothyronine in rats. Biull Eksp Biol Med. 1998;126:497-501.
22 Sun Y. Free radicals, antioxidant enzymes, and carcinogenesis. Free Radic Biol Med. 1990;8:583-599.   DOI   ScienceOn
23 El-Missiry MA, El-Sayed IH, Othman AI. Protection by metal complexes with SOD-mimetic activity against oxidative gastric injury induced by indomethacin and ethanol in rats. Ann Clin Biochem. 2001;38:694-700.   DOI   ScienceOn
24 Motilva V, Martín MJ, Luque MI, Alarcon de la Lastra C. Role of polymorphonuclear leukocytes and oxygen-derived free radicals in chronic gastric lesion induced by acetic acid in rat. Gen Pharmacol. 1996;27:545-550.   DOI   ScienceOn
25 Zimmerman BJ, Granger DN. Oxygen free radicals and the gastrointestinal tract: role in ischemia-reperfusion injury. Hepatogastroenterology. 1994;41:337-342.
26 Naito Y, Yoshikawa T, Yoshida N, Kondo M. Role of oxygen radical and lipid peroxidation in indomethacin-induced gastric mucosal injury. Dig Dis Sci. 1998;43(9 Suppl):30S-34S.
27 Takeuchi K, Ueshima K, Hironaka Y, Fujioka Y, Matsumoto J, Okabe S. Oxygen free radicals and lipid peroxidation in the pathogenesis of gastric mucosal lesions induced by indomethacin in rats. Relation to gastric hypermotility. Digestion. 1991;49:175-184.   DOI   ScienceOn
28 Tanaka J, Yuda Y. Lipid peroxidation in gastric mucosal lesions induced by indomethacin in rat. Biol Pharm Bull. 1996;19: 716-720.   DOI   ScienceOn
29 Lee JM, Im WJ, Nam YJ, Oh KH, Lim JC, Whang WK, Sohn UD. Acute toxicity and general pharmacological action of QGC EXT. Korean J Physiol Pharmacol. 2012;16:49-57.   DOI   ScienceOn
30 Middleton E Jr, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000;52:673-751.
31 Min YS, Lee SE, Hong ST, Kim HS, Choi BC, Sim SS, Whang WK, Sohn UD. The inhibitory effect of quercetin-3-O-beta-Dglucuronopyranoside on gastritis and reflux esophagitis in rats. Korean J Physiol Pharmacol. 2009;13:295-300.   DOI   ScienceOn
32 Kim JS, Song HJ, Ko SK, Whang WK, Sohn UD. Quercetin-3- O-beta-d-glucuronopyranoside (QGC)-induced HO-1 expression through ERK and PI3K activation in cultured feline esophageal epithelial cells. Fitoterapia. 2010;81:85-92.   DOI   ScienceOn
33 Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302-310.
34 Grisham MB, Benoit JN, Granger DN. Assessment of leukocyte involvement during ischemia and reperfusion of intestine. Methods Enzymol. 1990;186:729-42.
35 Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001;74:418-425.
36 Kostyuk VA, Potapovich AI, Speransky SD, Maslova GT. ProtecProtective effect of natural flavonoids on rat peritoneal macrophages injury caused by asbestos fibers. Free Radic Biol Med. 1996;21:487-493.   DOI   ScienceOn
37 Martin MJ, La-Casa C, Alarcon-de-la-Lastra C, Cabeza J, Villegas I, Motilva V. Anti-oxidant mechanisms involved in gastroprotective effects of quercetin. Z Naturforsch C. 1998;53:82-88.
38 Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol. 1988;37:837-841.   DOI   ScienceOn