Browse > Article
http://dx.doi.org/10.4196/kjpp.2012.16.2.139

TRPC-Mediated Current Is Not Involved in Endocannabinoid-Induced Short-Term Depression in Cerebellum  

Chang, Won-Seok (Department of Physiology, Seoul National University College of Medicine)
Kim, Jun (Department of Physiology, Seoul National University College of Medicine)
Kim, Sang-Jeong (Department of Physiology, Seoul National University College of Medicine)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.16, no.2, 2012 , pp. 139-144 More about this Journal
Abstract
It has been reported that activation of metabotropic glutamate receptor 1 (mGluR1) can mediate endocannabinoid-induced short-term depression of synaptic transmission in cerebellar parallel fiber (PF)-Purkinje cell (PC) synapse. mGluR1 has signaling pathways involved in intracellular calcium increase which may contribute to endocannabinoid release. Two major mGluR1-evoked calcium signaling pathways are known: (1) slow-kinetic inward current carried by transient receptor potential canonical (TRPC) channel which is permeable to $Ca^{2+}$; (2) $IP_3$-induced calcium release from intracellular calcium store. However, it is unclear how much each calcium source contributes to endocannabinoid signaling. Here, we investigated whether calcium influx through mGluR1-evoked TRPC channel contributes to endocannabinoid signaling in cerebellar Purkinje cells. At first, we applied SKF96365 to inhibit TRPC, which blocked endocannabinoid-induced short-term depression completely. However, an alternative TRP channel inhibitor, BTP2 did not affect endocannabinoid-induced short-term depression although it blocked mGluR1-evoked TRPC currents. Endocannabinoid signaling occurred normally even though the TRPC current was mostly blocked by BTP2. Our data imply that TRPC current does not play an important role in endocannabinoid signaling. We also suggest precaution in applying SKF96365 to inhibit TRP channels and propose BTP2 as an alternative TRPC inhibitor.
Keywords
Endocannabinoid; TRPC; Short-term depression; Cerebellar Purkinje cell; BTP2;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Schwarz G, Droogmans G, Nilius B. Multiple effects of SK&F 96365 on ionic currents and intracellular calcium in human endothelial cells. Cell Calcium. 1994;15:45-54.   DOI   ScienceOn
2 Capdevila J, Gil L, Orellana M, Marnett LJ, Mason JI, Yadagiri P, Falck JR. Inhibitors of cytochrome P-450-dependent arachidonic acid metabolism. Arch Biochem Biophys. 1988;261:257-263.   DOI   ScienceOn
3 Rodrigues AD, Gibson GG, Ioannides C, Parke DV. Interactions of imidazole antifungal agents with purified cytochrome P-450 proteins. Biochem Pharmacol. 1987;36:4277-4281.   DOI   ScienceOn
4 Mason MJ, Mayer B, Hymel LJ. Inhibition of $Ca^{2+}$ transport pathways in thymic lymphocytes by econazole, miconazole, and SKF 96365. Am J Physiol. 1993;264:C654-662.   DOI
5 Ishikawa J, Ohga K, Yoshino T, Takezawa R, Ichikawa A, Kubota H, Yamada T. A pyrazole derivative, YM-58483, potently inhibits store-operated sustained $Ca^{2+}$ influx and IL-2 production in T lymphocytes. J Immunol. 2003;170: 4441-4449.   DOI
6 Zitt C, Strauss B, Schwarz EC, Spaeth N, Rast G, Hatzelmann A, Hoth M. Potent inhibition of $Ca^{2+}$ release-activated $Ca^{2+}$ channels and T-lymphocyte activation by the pyrazole derivative BTP2. J Biol Chem. 2004;279:12427-12437.   DOI
7 Yoshida T, Hashimoto K, Zimmer A, Maejima T, Araishi K, Kano M. The cannabinoid CB1 receptor mediates retrograde signals for depolarization-induced suppression of inhibition in cerebellar Purkinje cells. J Neurosci. 2002;22:1690-1697.
8 Maejima T, Oka S, Hashimotodani Y, Ohno-Shosaku T, Aiba A, Wu D, Waku K, Sugiura T, Kano M. Synaptically driven endocannabinoid release requires $Ca^{2+}$-assisted metabotropic glutamate receptor subtype 1 to phospholipase Cbeta4 signaling cascade in the cerebellum. J Neurosci. 2005;25:6826-6835.   DOI   ScienceOn
9 Safo P, Regehr WG. Timing dependence of the induction of cerebellar LTD. Neuropharmacology. 2008;54:213-218.   DOI   ScienceOn
10 Pitler TA, Alger BE. Depolarization-induced suppression of GABAergic inhibition in rat hippocampal pyramidal cells: G protein involvement in a presynaptic mechanism. Neuron. 1994;13:1447-1455.   DOI   ScienceOn
11 Wagner JJ, Alger BE. Increased neuronal excitability during depolarization-induced suppression of inhibition in rat hippocampus. J Physiol. 1996;495:107-112.
12 Ohno-Shosaku T, Shosaku J, Tsubokawa H, Kano M. Cooperative endocannabinoid production by neuronal depolarization and group I metabotropic glutamate receptor activation. Eur J Neurosci. 2002;15:953-961.   DOI   ScienceOn
13 Merritt JE, Armstrong WP, Benham CD, Hallam TJ, Jacob R, Jaxa-Chamiec A, Leigh BK, McCarthy SA, Moores KE, Rink TJ. SK&F 96365, a novel inhibitor of receptor-mediated calcium entry. Biochem J. 1990;271:515-522.   DOI
14 Kim JH, PE, Chang DJ, Choi SW. Modulation of amygdala synaptic transmission by metabotropic glutamate receptors. Korean J Pharmacol. 2003;7:303-306.
15 Brenowitz SD, Regehr WG. Associative short-term synaptic plasticity mediated by endocannabinoids. Neuron. 2005;45:419-431.   DOI   ScienceOn
16 Hartmann J, Konnerth A. Mechanisms of metabotropic glutamate receptor-mediated synaptic signaling in cerebellar Purkinje cells. Acta Physiol (Oxf). 2008. [Epub ahead of print]
17 Singh A, Hildebrand ME, Garcia E, Snutch TP. The transient receptor potential channel antagonist SKF96365 is a potent blocker of low-voltage-activated T-type calcium channels. Br J Pharmacol. 2010;160:1464-1475.   DOI   ScienceOn
18 He LP, Hewavitharana T, Soboloff J, Spassova MA, Gill DL. A functional link between store-operated and TRPC channels revealed by the 3,5-bis(trifluoromethyl)pyrazole derivative, BTP2. J Biol Chem. 2005;280:10997-11006.   DOI   ScienceOn
19 Harper JL, Daly JW. Effect of calmidazolium analogs on calcium influx in HL-60 cells. Biochem Pharmacol. 2000;60:317-324.   DOI   ScienceOn
20 Ciardo A, Meldolesi J. Multiple actions of SC 38249: the blocker of both voltage-operated and second messenger-operated $Ca^{2+}$ channels also inhibits $Ca^{2+}$ extrusion. Eur J Pharmacol. 1990;188:417-421.   DOI   ScienceOn
21 Alvarez J, Montero M, Garcia-Sancho J. High affinity inhibition of $Ca^{2+}$-dependent $K^+$ channels by cytochrome P-450 inhibitors. J Biol Chem. 1992;267:11789-11793.
22 Mockett BG, Guevremont D, Wutte M, Hulme SR, Williams JM, Abraham WC. Calcium/calmodulin-dependent protein kinase II mediates group I metabotropic glutamate receptor-dependent protein synthesis and long-term depression in rat hippocampus. J Neurosci. 2011;31:7380-7391.   DOI   ScienceOn
23 Conn PJ, Pin JP. Pharmacology and functions of metabotropic glutamate receptors. Annu Rev Pharmacol Toxicol. 1997;37:205-237.   DOI   ScienceOn
24 Nakanishi S. Metabotropic glutamate receptors: synaptic transmission, modulation, and plasticity. Neuron. 1994;13:1031-1037.   DOI   ScienceOn
25 Lee J, Kim I, Oh SR, Ko SJ, Lim MK, Kim DG, Kim CH. Regulation of DREAM expression by group I mGluR. Korean J Physiol Pharmacol. 2011;15:95-100.   DOI   ScienceOn
26 Takechi H, Eilers J, Konnerth A. A new class of synaptic response involving calcium release in dendritic spines. Nature. 1998;396:757-760.   DOI   ScienceOn
27 Finch EA, Augustine GJ. Local calcium signalling by inositol-1,4,5-trisphosphate in Purkinje cell dendrites. Nature. 1998;396:753-756.   DOI   ScienceOn
28 Tempia F, Miniaci MC, Anchisi D, Strata P. Postsynaptic current mediated by metabotropic glutamate receptors in cerebellar Purkinje cells. J Neurophysiol. 1998;80:520-528.   DOI
29 Kim SJ, Kim YS, Yuan JP, Petralia RS, Worley PF, Linden DJ. Activation of the TRPC1 cation channel by metabotropic glutamate receptor mGluR1. Nature. 2003;426:285-291.   DOI   ScienceOn
30 Chuang SC, Bianchi R, Wong RK. Group I mGluR activation turns on a voltage-gated inward current in hippocampal pyramidal cells. J Neurophysiol. 2000;83:2844-2853.   DOI
31 Devane WA, Hanus L, Breuer A, Pertwee RG, Stevenson LA, Griffin G, Gibson D, Mandelbaum A, Etinger A, Mechoulam R. Isolation and structure of a brain constituent that binds to the cannabinoid receptor. Science. 1992;258:1946-1949.   DOI
32 Matsuda LA, Lolait SJ, Brownstein MJ, Young AC, Bonner TI. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature. 1990;346:561-564.   DOI   ScienceOn
33 Caulfield MP, Brown DA. Cannabinoid receptor agonists inhibit Ca current in NG108-15 neuroblastoma cells via a pertussis toxin-sensitive mechanism. Br J Pharmacol. 1992;106:231-232.   DOI   ScienceOn
34 Herkenham M, Lynn AB, Little MD, Johnson MR, Melvin LS, de Costa BR, Rice KC. Cannabinoid receptor localization in brain. Proc Natl Acad Sci USA. 1990;87:1932-1936.   DOI   ScienceOn
35 Stella N, Schweitzer P, Piomelli D. A second endogenous cannabinoid that modulates long-term potentiation. Nature. 1997;388:773-778.   DOI   ScienceOn