Browse > Article
http://dx.doi.org/10.4196/kjpp.2011.15.5.267

Characteristics of 5-Hydroxytryptamine Receptors Involved in Contraction of Feline Ileal Longitudinal Smooth Muscle  

Wang, Yiyi (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Park, Sun-Young (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Oh, Kyung-Hoon (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Min, Young-Sil (Faculty of Herb Industry, Jungwon University)
Lee, Yun-Jeong (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Lee, Seok-Yong (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.15, no.5, 2011 , pp. 267-272 More about this Journal
Abstract
A number of studies have demonstrated that 5-hydroxytryptamine (5-HT) can induce muscle contraction or relaxation response and enhance secretion in the gastrointestinal tract via a multiplicity of 5-HT receptor subtypes. In the present study, we investigated the pharmacological characterization of the 5-HT-induced contractile response in longitudinal smooth muscle isolated from the feline ileum. Addition of 5-HT into muscle chambers enhanced the basal tone and spontaneous activity in a concentration-dependent manner. The neurotoxin tetrodotoxin did not alter the 5-HT-induced contraction of the longitudinal muscles. Neither atropine nor guanethidine affected the contraction. The 5-HT agonists, 5-methylserotonin hydrochloride and mosapride, also evoked concentration-dependent contractions. The 5-HT-induced contraction was enhanced by the $5HT_2$ receptor antagonist ketanserin and the $5-HT_3$ receptor antagonist ondansetron but was inhibited by the 5-$HT_1$ receptor antagonist methysergide and 5-$HT_4$ receptor antagonist GR113808. These results indicate that 5-$HT_1$ and 5-$HT_4$ receptors may mediate the contraction of the 5-HT-induced response and 5-$HT_2$ and 5-$HT_3$ receptors may mediate 5-HT-induced relaxation in feline ileal longitudinal smooth muscles.
Keywords
Ileum; Longitudinal smooth muscle; 5-HT; Contraction; Relaxation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Buchheit KH, Engel G, Mutschler E, Richardson B. Study of the contractile effect of 5-hydroxytryptamine (5-HT) in the isolated longitudinal muscle strip from guinea-pig ileum. Evidence for two distinct release mechanisms. Naunyn Schmiedebergs Arch Pharmacol. 1985;329:36-41.   DOI   ScienceOn
2 Gaddum JH, Picarelli ZP. Two kinds of tryptamine receptor. Br J Pharmacol. 1957;12:323-328.
3 Yamano M, Ito H, Miyata K. Species differences in the 5-hydroxytryptamine- induced contraction in the isolated distal ileum. Jpn J Pharmacol. 1997;74:267-274.   DOI
4 Berger M, Gray JA, Roth BL. The expanded biology of serotonin. Annu Rev Med. 2009;60:355-366   DOI   ScienceOn
5 De Maeyer JH, Lefebvre RA, Schuurkes JA. 5-HT4 receptor agonists: similar but not the same. Neurogastroenterol Motil. 2008;20:99-112.   DOI   ScienceOn
6 Hannon J, Hoyer D. Molecular biology of 5-HT receptors. Behav Brain Res. 2008;195:198-213.   DOI   ScienceOn
7 Javid FA, Naylor RJ. Characterization of the 5-hydroxytryptamine receptors mediating contraction in the intestine of Suncus murinus. Br J Pharmacol. 1999;127:1867-1875.   DOI   ScienceOn
8 Kitazawa T, Ukai H, Komori S, Taneike T. Pharmacological characterization of 5-hydroxytryptamine-induced contraction in the chicken gastrointestinal tract. Auton Autacoid Pharmacol. 2006;26:157-168.   DOI   ScienceOn
9 Komada T, Yano S. Pharmacological characterization of 5-Hydroxytryptamine- receptor subtypes in circular muscle from the rat stomach. Biol Pharm Bull. 2007;30:508-513.   DOI   ScienceOn
10 Tuladhar BR, Costall B, Naylor RJ. Pharmacological characterization of the 5-hydroxytryptamine receptor mediating relaxation in the rat isolated ileum. Br J Pharmacol. 1996;119: 303-310   DOI   ScienceOn
11 Villalon CM, den Boer MO, Heiligers JP, Saxena PR. Mediation of 5-hydroxytryptamine-induced tachycardia in the pig by the putative 5-HT4 receptor. Br J Pharmacol. 1990;100:665-667   DOI   ScienceOn
12 Bush TG, Spencer NJ, Watters N, Sanders KM, Smith TK. Effects of alosetron on spontaneous migrating motor complexes in murine small and large bowel in vitro. Am J Physiol Gastrointest Liver Physiol. 2001;281:G974-G983.   DOI
13 Park SY, Shim JH, Kim M, Sun YH, Kwak HS, Yan X, Choi BC, Im C, Sim SS, Jeong JH, Kim IK, Min YS, Sohn UD. MLCK and PKC Involvements via Gi and Rho A protein in contraction by the electrical field stimulation in feline esophageal smooth muscle. Korean J Physiol Pharmacol. 2010;14:29-35.   DOI
14 Saxena PR. Cardiovascular effects from stimulation of 5-hydroxytryptamine receptors. Fundam Clin Pharmacol. 1989;3: 245-265.   DOI   ScienceOn
15 Wilson H, Coffman WJ, Cohen ML. 5-Hydroxytryptamine3 receptors mediate tachycardia in conscious instrumented dogs. J Pharmacol Exp Ther. 1990;252:683-688.
16 Sanders KM. A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract. Gastroenterology. 1996;111:492-515.   DOI   ScienceOn
17 Tuladhar BR, Womack MD, Naylor RJ. Pharmacological characterization of the 5-HT receptor-mediated contraction in the mouse isolated ileum. Br J Pharmacol. 2000;131:1716-1722   DOI   ScienceOn
18 Tam FS, Hillier K, Bunce KT. Characterization of the 5-hydroxytryptamine receptor type involved in inhibition of spontaneous activity of human isolated colonic circular muscle. Br J Pharmacol. 1994;113:143-150   DOI   ScienceOn
19 Alberti E, Mikkelsen HB, Larsen JO, Jimenez M. Motility patterns and distribution of interstitial cells of Cajal and nitrergic neurons in the proximal, mid- and distal-colon of the rat. Neurogastroenterol Motil. 2005;17:133-147.   DOI   ScienceOn
20 Thomsen L, Robinson TL, Lee JC, Farraway LA, Hughes MJ, Andrews DW, Huizinga JD. Interstitial cells of Cajal generate a rhythmic pacemaker current. Nat Med. 1998;4:848-851.   DOI   ScienceOn
21 Bearcroft CP, Perrett D, Farthing MJ. Postprandial plasma 5-hydroxytryptamine in diarrhoea predominant irritable bowel syndrome: a pilot study. Gut. 1998;42:42-46.   DOI   ScienceOn
22 Park SY, Je HD, Shim JH, Sohn UD. Characteristics of spontaneous contraction in the circular smooth muscles of cat ileum. Arch Pharm Res. 2010;33:159-165.   DOI
23 Gonzalez A, Sarna SK. Neural regulation of in vitro giant contractions in the rat colon. Am J Physiol Gastrointest Liver Physiol. 2001;281:G275-G282   DOI
24 Kim DY, Camilleri M. Serotonin: a mediator of the brain-gut connection. Am J Gastroenterol. 2000;95:2698-2709.
25 Woollard DJ, Bornstein JC, Furness JB. Characterization of 5-HT receptors mediating contraction and relaxation of the longitudinal muscle of guinea-pig distal colon in vitro. Naunyn Schmiedebergs Arch Pharmacol. 1994;349:455-462.   DOI   ScienceOn
26 Gershon MD, Tack J. The serotonin signaling system: from basic understanding to drug development for functional GI disorders. Gastroenterology. 2007;132:397-414.   DOI   ScienceOn
27 Gershon MD. Review article: roles played by 5-hydroxytryptamine in the physiology of the bowel. Aliment Pharmacol Ther. 1999;13 Suppl 2:15-30.
28 Atkinson W, Lockhart S, Whorwell PJ, Keevil B, Houghton LA. Altered 5-hydroxytryptamine signaling in patients with constipation- and diarrhea-predominant irritable bowel syndrome. Gastroenterology. 2006;130:34-43.   DOI   ScienceOn
29 Dunlop SP, Coleman NS, Blackshaw E, Perkins AC, Singh G, Marsden CA, Spiller RC. Abnormalities of 5-hydroxytryptamine metabolism in irritable bowel syndrome. Clin Gastroenterol Hepatol. 2005;3:349-357.   DOI   ScienceOn
30 Houghton LA, Atkinson W, Whitaker RP, Whorwell PJ, Rimmer MJ. Increased platelet depleted plasma 5-hydroxytryptamine concentration following meal ingestion in symptomatic female subjects with diarrhoea predominant irritable bowel syndrome. Gut. 2003;52:663-670.   DOI   ScienceOn
31 Sikander A, Rana SV, Prasad KK. Role of serotonin in gastrointestinal motility and irritable bowel syndrome. Clin Chim Acta. 2009;403:47-55.   DOI   ScienceOn
32 Foong JP, Parry LJ, Gwynne RM, Bornstein JC. 5-HT(1A), SST(1), and SST(2) receptors mediate inhibitory postsynaptic potentials in the submucous plexus of the guinea pig ileum. Am J Physiol Gastrointest Liver Physiol. 2010;298:G384-G394   DOI   ScienceOn
33 Janssen P, Prins NH, Meulemans AL, Lefebvre RA. Smooth muscle 5-HT2A receptors mediating contraction of porcine isolated proximal stomach strips. Br J Pharmacol. 2002;137: 1217-1224.   DOI   ScienceOn
34 Ramirez MJ, Del Rio J, Cenarruzabeitia E, Lasheras B. On the nature of the 5-HT receptor subtype inhibiting acetylcholine release in the guinea-pig ileum. Br J Pharmacol. 1994;113: 77-80.   DOI   ScienceOn