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http://dx.doi.org/10.4196/kjpp.2011.15.1.37

Spontaneous Contractions Augmented by Cholinergic and Adrenergic Systems in the Human Ureter  

Lee, Hyun-Woo (Department of Life and Leisure Sport/Sport Industry, College of Sports Science)
Baak, Cheol-Hee (Department of Physiology, College of Medicine, Chung-Ang University)
Lee, Moo-Yeol (Department of Physiology, College of Medicine, Chung-Ang University)
Kim, Young-Chul (Department of Physiology, College of Medicine, Chungbuk National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.15, no.1, 2011 , pp. 37-41 More about this Journal
Abstract
Interstitial cells of Cajal (ICC) evoke pacemaker activities in many tissues. The purpose of this study was to investigate the relationship between interstitial cell and pacemaker activity in the human ureter through the recording of spontaneous contractions. Spontaneous contractions of eight circular and longitudinal smooth muscle strips of the human ureter to acetylcholine (ACh) and/or norepinephrine (NE) were observed. Human ureteral strips were divided into proximal and distal groups, and each group was subdivided into circular and longitudinal groups. The proximal group showed spontaneous activities of 3~4 times within 5 minutes in the longitudinal group. ACh ($10^{-4}\;M$) augmented the frequency of the spontaneous contractions. The cumulative application of NE also augmented the frequency in a dose-dependent manner. The effects of NE application were inhibited by concomitant application of $10^{-5}\;M$ glibenclamide. Receptor tyrosine kinase (c-kit) staining revealed abundant ICCs only in proximal tissues. Therefore, spontaneous contractions of the human ureter might be modulated by ICC in the proximal region, and the actions might be related with the activation of cholinergic and/or adrenergic system mediated by a glibenclamide-sensitive pathway.
Keywords
Interstitial cell of Cajal; Pacemaker activity; Spontaneous contraction; Human ureter;
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1 Jun JY, Choi S, Yeum CH, Chang IY, Park CK, Kim MY, Kong ID, So I, Kim KW, You HJ. N oradrenaline inhibits pacemaker currents through stimulation of beta 1-adrenoceptors in cultured interstitial cells of Cajal from murine small intestine. Br J Pharmacol. 2004;141:670-677.   DOI   ScienceOn
2 Oostendorp J, Preitner F, Moffatt J, Jimenez M, Giacobino JP, Molenaar P, Kaumann AJ. Contribution of beta-adrenoceptor subtypes to relaxation of colon and oesophagus and pacemaker activity of ureter in wildtype and beta(3)-adrenoceptor knockout mice. Br J Pharmacol. 2000;130:747-758.   DOI   ScienceOn
3 Satoh H. Effects of ATP-sensitive $K^+$ channel openers on pacemaker activity in isolated single rabbit sino-atrial node cells. J Cardiovasc Pharmacol. 1993;22:863-868.   DOI   ScienceOn
4 Hatton WJ, Mason HS, Carl A, Doherty P, Latten MJ, Kenyon JL, Sanders KM, Horowitz B. Functional and molecular expression of a voltage-dependent K(+) channel (Kv1.1) in interstitial cells of Cajal. J Physiol. 2001;533:315-327.   DOI   ScienceOn
5 Narishige T, Egashira K, Akatsuka Y, Katsuda Y, Numaguchi K, Sakata M, Takeshita A. Glibenclamide, a putative ATPsensitive $K^+$ channel blocker, inhibits coronary autoregulation in anesthetized dogs. Circ Res. 1993;73:771-776.   DOI   ScienceOn
6 Pfister SL, Pratt PE, Kurian J, Campbell WB. Glibenclamide inhibits thromboxane-mediated vasoconstriction by thromboxane receptor blockade. Vascul Pharmacol. 2004;40:285-292.   DOI   ScienceOn
7 Lang RJ, Tonta MA, Zoltkowski BZ, Meeker WF, Wendt I, Parkington HC. Pyeloureteric peristalsis: role of atypical smooth muscle cells and interstitial cells of Cajal-like cells as pacemakers. J Physiol. 2006;576:695-705.   DOI   ScienceOn
8 Lang RJ, Zoltkowski BZ, Hammer JM, Meeker WF, Wendt I. Electrical characterization of interstitial cells of Cajal-like cells and smooth muscle cells isolated from the mouse ureteropelvic junction. J Urol. 2007;177:1573-1580.   DOI   ScienceOn
9 Biers SM, Reynard JM, Doore T, Brading AF. The functional effects of a c-kit tyrosine inhibitor on guinea-pig and human detrusor. BJU Int. 2006;97:612-616.   DOI   ScienceOn
10 Metzger R, Schuster T, Till H, Stehr M, Franke FE, Dietz HG. Cajal-like cells in the human upper urinary tract. J Urol. 2004;172:769-772.   DOI   ScienceOn
11 Torihashi S, Ward SM, Nishikawa S, Nishi K, Kobayashi S, Sanders KM. c-kit-dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract. Cell Tissue Res. 1995;280:97-111.
12 Pezzone MA, Watkins SC, Alber SM, King WE, de Groat WC, Chancellor MB, Fraser MO. Identification of c-kit-positive cells in the mouse ureter: the interstitial cells of Cajal of the urinary tract. Am J Physiol Renal Physiol. 2003;284:F925-F929.   DOI
13 Isozaki K, Hirota S, Miyagawa J, Taniguchi M, Shinomura Y, Matsuzawa Y. Deficiency of c-kit+ cells in patients with a myopathic form of chronic idiopathic intestinal pseudoobstruction. Am J Gastroenterol. 1997;92:332-334.
14 Vanderwinden JM, Rumessen JJ. Interstitial cells of Cajal in human gut and gastrointestinal disease. Microsc Res Tech. 1999;47:344-360.   DOI   ScienceOn
15 Yamataka A, Kato Y, Tibboel D, Murata Y, Sueyoshi N, Fujimoto T, Nishiye H, Miyano T. A lack of intestinal pacemaker (c-kit) in aganglionic bowel of patients with Hirschsprung's disease. J Pediatr Surg. 1995;30:441-444.   DOI   ScienceOn
16 Kim JK, Han WH, Lee MY, Myung SC, Kim SC, Kim MK. Testosterone relaxes rabbit seminal vesicle by calcium channel inhibition. Korean J Physiol Pharmacol. 2008;12:73-77.   DOI
17 Ogasawara N, Tsukamoto T, Inada K, Mizoshita T, Ban N, Yamao K, Joh T, Itoh M, Tatematsu M. Frequent c-Kit gene mutations not only in gastrointestinal stromal tumors but also in interstitial cells of Cajal in surrounding normal mucosa. Cancer Lett. 2005;230:199-210.   DOI   ScienceOn
18 Somlyo AP, Somlyo AV. Signal transduction and regulation in smooth muscle. Nature. 1994;372:231-236.   DOI   ScienceOn
19 Hille B. Ionic channels of excitable membranes. 1st ed. Sinauer: Sunderland; c1992. 119-244 p.
20 Park SY, Lee MY, Keum EM, Myung SC, Kim SC. Ionic currents in single smooth muscle cells of the human vas deferens. J Urol. 2004;172:628-633.   DOI   ScienceOn
21 Cajal SR. Histologie du systeme nerveux de l'homme et des vertebres. Paris, Maloine. 1911;2:891-942.
22 Langton P, Ward SM, Carl A, Norell MA, Sanders KM. Spontaneous electrical activity of interstitial cells of Cajal isolated from canine proximal colon. Proc Natl Acad Sci U S A. 1989;86:7280-7284.   DOI   ScienceOn
23 McCloskey KD, Gurney AM. Kit positive cells in the guinea pig bladder. J Urol. 2002;168:832-836.   DOI   ScienceOn
24 Ward SM, Burns AJ, Torihashi S, Sanders KM. Mutation of the proto-oncogene c-kit blocks development of interstitial cells and electrical rhythmicity in murine intestine. J Physiol. 1994;480:91-97.