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http://dx.doi.org/10.4196/kjpp.2010.14.3.163

Morinda citrifolia Inhibits Both Cytosolic $Ca^{2+}$-dependent Phospholipase $A_2$ and Secretory $Ca^{2+}$-dependent Phospholipase $A_2$  

Song, Ho-Sun (Department of Pathophysiology, College of Pharmacy, Chung-Ang University)
Park, Sung-Hun (Department of Pathophysiology, College of Pharmacy, Chung-Ang University)
Ko, Myoung-Soo (Department of Pathophysiology, College of Pharmacy, Chung-Ang University)
Jeong, Jae-Min (Department of Pathophysiology, College of Pharmacy, Chung-Ang University)
Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
Sim, Sang-Soo (Department of Pathophysiology, College of Pharmacy, Chung-Ang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.14, no.3, 2010 , pp. 163-167 More about this Journal
Abstract
This study investigated the effects of the methanol extracts of Morinda citrifolia containing numerous anthraquinone and iridoid on phospholipase $A_2$ ($PLA_2$) isozyme. $PLA_2$ activity was measured using various $PLA_2$ substrates, including 10-pyrene phosphatidylcholine, 1-palmitoyl-2-[$^{14}C$]arachidonyl phosphatidylcholine ([$^{14}C$]AA-PC), and [$^3H$]arachidonic acid (AA). The methanol extracts suppressed melittin-induced [$_3H$]AA release in a concentration-dependent manner in RAW 264.7 cells, and inhibited $cPLA_2/sPLA_2$-induced hydrolysis of [$^{14}C$]AA-PC in a concentration- and time-dependent manner. A Dixon plot showed that the inhibition by methanol extracts on $cPLA_2$ and $sPLA_2$ appeared to be competitive with inhibition constants ($K_i$) of $3.7{\mu}g/ml$ and $12.6{\mu}g/ml$, respectively. These data suggest that methanol extracts of Morinda citrifolia inhibits both $Ca^{2+}$-dependent $PLA_2$ such as, $cPLA_2$ and $sPLA_2$. Therefore, Morinda citrifolia may possess anti-inflammatory activity secondary to $Ca^{2+}$-dependent $PLA_2$ inhibition.
Keywords
Morinda citrifolia; Phospholipase $A_2$; Arachidonic acid;
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