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http://dx.doi.org/10.4196/kjpp.2009.13.6.443

Electrically-evoked Neural Activities of rd1 Mice Retinal Ganglion Cells by Repetitive Pulse Stimulation  

Ryu, Sang-Baek (Department of Biomedical Engineering, College of Health Science, Yonsei University)
Ye, Jang-Hee (Department of Physiology, Chungbuk National University School of Medicine)
Lee, Jong-Seung (Department of Biomedical Engineering, College of Health Science, Yonsei University)
Goo, Yong-Sook (Department of Physiology, Chungbuk National University School of Medicine)
Kim, Chi-Hyun (Department of Biomedical Engineering, College of Health Science, Yonsei University)
Kim, Kyung-Hwan (Department of Biomedical Engineering, College of Health Science, Yonsei University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.13, no.6, 2009 , pp. 443-448 More about this Journal
Abstract
For successful visual perception by visual prosthesis using electrical stimulation, it is essential to develop an effective stimulation strategy based on understanding of retinal ganglion cell (RGC) responses to electrical stimulation. We studied RGC responses to repetitive electrical stimulation pulses to develop a stimulation strategy using stimulation pulse frequency modulation. Retinal patches of photoreceptor-degenerated retinas from rd1 mice were attached to a planar multi-electrode array (MEA) and RGC spike trains responding to electrical stimulation pulse trains with various pulse frequencies were observed. RGC responses were strongly dependent on inter-pulse interval when it was varied from 500 to 10 ms. Although the evoked spikes were suppressed with increasing pulse rate, the number of evoked spikes were >60% of the maximal responses when the inter-pulse intervals exceeded 100 ms. Based on this, we investigated the modulation of evoked RGC firing rates while increasing the pulse frequency from 1 to 10 pulses per second (or Hz) to deduce the optimal pulse frequency range for modulation of RGC response strength. RGC response strength monotonically and linearly increased within the stimulation frequency of 1~9 Hz. The results suggest that the evoked neural activities of RGCs in degenerated retina can be reliably controlled by pulse frequency modulation, and may be used as a stimulation strategy for visual neural prosthesis.
Keywords
Degenerated retina; Microelectrode arrray (MEA); Retinal ganglion cells; Retinal implant; Electrical stimulation; Visual neural prosthesis;
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1 Hornig R, Laube T, Walter P, Velikay-Parel M, Bornfeld N, Feucht M, Akguel H, Rössler G, Alteheld N, Notarp DL, Wyatt J, Richard G. A method and technical equipment for an acute human trial to evaluate retinal implant technology. J Neural Eng 2: S129-S124, 2005   DOI   ScienceOn
2 Humayun MS, Weiland JD, Fujii GY, Greenberg R, Williamson R, Little J, Mech B, Cimmarusti V, Boemel GV, Dagnelie G, de Juan E Jr. Visual perception in a blind subject with a chronic microelectronic retinal prosthesis. Vision Res 43: 2573-2581, 2003   DOI   ScienceOn
3 Rizzo JF III, Wyatt J, Loewenstein J, Kelly S, Shire D. Perceptual efficacy of electrical stimulation of human retina with a microelectrode array during short-term surgical trials. Invest Ophth Vis Sci 44: 5362-5369, 2003   DOI   ScienceOn
4 Sekirnjak C, Hottowy P, Sher A, Dabrowski W, Litke AM, Chichilnisky EJ. Electrical stimulation of mammalian retinal ganglion cells with multielectrode arrays. J Neurophysiol 95: 3311-3327, 2006   DOI   ScienceOn
5 Ye JH, Goo YS. The slow wave component of retinal activity in rd/rd mouse recorded with a multi-electrode array. Physiol Meas 28: 1079-1088, 2007   DOI   ScienceOn
6 Zrenner E. Will retinal implants restore vision? Science 295: 1022-1025, 2002   DOI   PUBMED   ScienceOn
7 Merabet LB, Rizzo JF, Amedi A, Somers DC, Pascual-Leone A. What blindness can tell us about seeing again: merging neuroplasticity and neuroprosthesis. Nat Rev Neurosci 6: 71-77, 2005   DOI   PUBMED   ScienceOn
8 Ahuja AK, Behrend MR, Kuroda M, Humayun MS, Weiland JD. An in-vitro model of a retinal implant. IEEE Trans Biomed Eng 55: 1744-1753, 2008   DOI   ScienceOn
9 Jensen RJ, Rizzo JF III. Responses of ganglion cells to repetitive electrical stimulation of the retina. J Neural Eng 4: S1-S6, 2007   DOI   ScienceOn
10 Ryu SB, Ye JH, Lee JS, Goo YS, Kim KH. Characterization of retinal ganglion cell activities evoked by temporally patterned electrical stimulation for the development of stimulus encoding strategies for retinal implants. Brain Res 1275: 33-42, 2009b   DOI   PUBMED   ScienceOn
11 Fried SI, Hsueh HA, Werblin FS. A method for generating precise temporal patterns of retinal spiking using prosthetic stimulation. J Neurophysiol 95: 970-978, 2006   DOI   ScienceOn
12 Humayun MS, de Juan E Jr, Weiland JD, Dangnelie G, Katona S, Greenberg R, Suzuki S. Pattern electrical stimulation of the human retina. Vision Res 39: 2569-2576, 1999   DOI   ScienceOn
13 Stett A, Barth W, Weiss S, Haemmerle H, Zrenner E. Electrical multisite stimulation of the isolated chicken retina. Vis Res 40: 1785-1795, 2000   DOI   ScienceOn
14 Hesse L, Schanze T, Wilms M, Eger M. Implantation of retina stimulation electrodes and recording of electrical stimulation responses in the visual cortex of the cat. Graef Arch Clin Exp Ophthal 238: 840-845, 2000   DOI   ScienceOn
15 Sivaprakasam M, Wentai L, Guoxing W, Weiland JD, Humayun MS. Architecture tradeoffs in high-density microstimulators for retinal prosthesis. IEEE Trans Circuits Syst I-Regul Pap 52: 2629-2641, 2005   DOI   ScienceOn
16 Kazemi M, Basham E, Sivaprakasam M, Wang G, Rodger D, Weiland J, Tai YC, Liu W, Humayun M. A test microchip for evaluation of hermetic packaging technology for biomedical prosthetic implants. Conf Proc IEEE Eng Med Biol Soc 6: 4093-4095, 2004   PUBMED
17 Jensen RJ, Ziv OR, Rizzo JF. Responses of rabbit retinal ganglion cells to electrical stimulation with an epiretinal electrode. J Neural Eng 2: S16-S21, 2005   DOI   ScienceOn
18 Margolis DJ, Newkirk G, Euler T, Detwiler PB. Functional stability of retinal ganglion cells after degeneration-induced changes in synaptic input. J Neurosci 28: 6526-6536, 2008   DOI   ScienceOn
19 Veraart C, Raftopoulos C, Mortmer JT, Delbeke J, Pins D, Michaus G, Vanlierde A, Parrini S, Wanet-Defalqu M. Visual sensations produced by optic nerve stimulation using an implanted self-sizing spiral cuff electrode. Brain Res 813: 181-186, 1998   DOI   ScienceOn
20 Ryu SB, Lee JS, Ye JH, Goo YS, Kim CH, Kim KH. Analysis of neuronal activities of retinal ganglion cells of degenerated retinal evoked by electrical pulse stimulation. J Biomed Eng Res 30: 347-354, 2009a
21 Stasheff SF. Emergence of sustained spontaneous hyperactivity and temporary preservation of OFF responses in ganglion cells of the retinal degeneration (rd1) mouse. J Neurophysiol 99: 1408-1421, 2008   DOI   PUBMED   ScienceOn
22 Seo JM, Kim SJ, Chung H, Kim ET, Yu HG, Yu YS. Biocompatibility of polyimide microelectrode array for retinal stimulation. Mater Sci Eng C 24: 185-189, 2004   DOI   ScienceOn