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http://dx.doi.org/10.4196/kjpp.2009.13.6.431

Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase  

Lee, Kwon-Ho (Department of Physiology, School of Medicine, Chungnam National University)
Lee, Sang-Ki (Department of Physiology, School of Medicine, Chungnam National University)
Kim, Hyo-Shin (Department of Physiology, School of Medicine, Chungnam National University)
Cho, Eun-Jung (Department of Physiology, School of Medicine, Chungnam National University)
Joo, Hee-Kyoung (Department of Physiology, School of Medicine, Chungnam National University)
Lee, Eun-Ji (Department of Physiology, School of Medicine, Chungnam National University)
Lee, Ji-Young (Department of Physiology, School of Medicine, Chungnam National University)
Park, Myoung-Soo (Department of Physiology, School of Medicine, Chungnam National University)
Chang, Seok-Jong (Department of Physiology, College of Medicine, Seonam University)
Cho, Chung-Hyun (Department of Physiology, School of Medicine, Chungnam National University)
Park, Jin-Bong (Department of Physiology, School of Medicine, Chungnam National University)
Jeon, Byeong-Hwa (Department of Physiology, School of Medicine, Chungnam National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.13, no.6, 2009 , pp. 431-436 More about this Journal
Abstract
The role of apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1) on the lead (Pb)-induced cellular response was investigated in the cultured endothelial cells. Pb caused progressive cellular death in endothelial cells, which occurred in a concentration- and time-dependent manner. However, Ref-1 overexpression with AdRef-1 significantly inhibited Pb-induced cell death in the endothelial cells. Also the overexpression of Ref-1 significantly suppressed Pb-induced superoxide and hydrogen peroxide elevation in the endothelial cells. Pb exposure induced the downregulation of catalase, it was inhibited by the Ref-1 overexpression in the endothelial cells. Taken together, our data suggests that the overexpression of Ref-1 inhibited Pb-induced cell death via the upregulation of catalase in the cultured endothelial cells.
Keywords
Apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1); Lead; Cell death; Endothelial cells; Catalase;
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