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http://dx.doi.org/10.4196/kjpp.2009.13.6.409

Altered Gene Expression in Cerulein-Stimulated Pancreatic Acinar Cells: Pathologic Mechanism of Acute Pancreatitis  

Yu, Ji-Hoon (Department of Pharmacology, Yonsei University College of Medicine)
Lim, Joo-Weon (Department of Food and Nutrition, Research Institute of Food & Nutritional Sciences, Brain Korea 21 Project, Yonsei University College of Human Ecology)
Kim, Hye-Young (Department of Food and Nutrition, Research Institute of Food & Nutritional Sciences, Brain Korea 21 Project, Yonsei University College of Human Ecology)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.13, no.6, 2009 , pp. 409-416 More about this Journal
Abstract
Acute pancreatitis is a multifactorial disease associated with the premature activation of digestive enzymes. The genes expressed in pancreatic acinar cells determine the severity of the disease. The present study determined the differentially expressed genes in pancreatic acinar cells treated with cerulein as an in vitro model of acute pancreatitis. Pancreatic acinar AR42J cells were stimulated with $10^{-8}$ M cerulein for 4 h, and genes with altered expression were identified using a cDNA microarray for 4,000 rat genes and validated by real-time PCR. These genes showed a 2.5-fold or higher increase with cerulein: lithostatin, guanylate cyclase, myosin light chain kinase 2, cathepsin C, progestin-induced protein, and pancreatic trypsin 2. Stathin 1 and ribosomal protein S13 showed a 2.5-fold or higher decreases in expression. Real-time PCR analysis showed time-dependent alterations of these genes. Using commercially available antibodies specific for guanylate cyclase, myosin light chain kinase 2, and cathepsin C, a time-dependent increase in these proteins were observed by Western blotting. Thus, disturbances in proliferation, differentiation, cytoskeleton arrangement, enzyme activity, and secretion may be underlying mechanisms of acute pancreatitis.
Keywords
Cerulein; Pancreatitis; Acinar cells; DNA microarray;
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